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Support Films

  • Michael J. Dykstra
  • Laura E. Reuss

Abstract

Support films attached to the surface of grids can be used for negative staining or shadowing of particulate samples (mitochondria, microsomes, bacteria, viruses). The films serve as electron-transparent Substrates for these samples. In addition, grids with large amounts of open space (bar, hole, or slot grids) that otherwise would provide inadequate support for plastic sections can be coated with films so that large areas of sections, or the entire section in the case of slot and hole grids, may be viewed without grid bars obscuring section detail. Support films are usually made from Formvar, Butvar, collodion, or carbon.

Keywords

Carbon Film Plastic Film Vacuum Evaporator Interference Color Amyl Acetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Faberge, A.C. 1984. Formvar and butvar support films; some general considerations. Bull. Electron Microsc. Soc. Am. 14: 102.Google Scholar
  2. Hayat, M.A., and Miller, S.E. 1990. Negative staining. McGraw-Hill, New York.Google Scholar

Copyright information

© Michael J. Dykstra 2003

Authors and Affiliations

  • Michael J. Dykstra
    • 1
  • Laura E. Reuss
    • 1
  1. 1.North Carolina State UniversityUSA

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