Support films attached to the surface of grids can be used for negative staining or shadowing of particulate samples (mitochondria, microsomes, bacteria, viruses). The films serve as electron-transparent Substrates for these samples. In addition, grids with large amounts of open space (bar, hole, or slot grids) that otherwise would provide inadequate support for plastic sections can be coated with films so that large areas of sections, or the entire section in the case of slot and hole grids, may be viewed without grid bars obscuring section detail. Support films are usually made from Formvar, Butvar, collodion, or carbon.
KeywordsFormaldehyde Acetone Chloroform Wettability Grease
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