Changes in substrate metabolism in isolated mouse hearts following ischemia-reperfusion

  • Ellen Aasum
  • Anne D. Hafstad
  • Terje S. Larsen
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 42)

Abstract

Several genetic and transgenic mouse models are currently being used for studying the regulation of myocardial contractility under normal conditions and in disease states. Little information has been provided, however, about myocardial energy metabolism in mouse hearts. We measured glycolysis, glucose oxidation and palmitate oxidation (using 3H-glucose, 14C-glucose and3H-palmitate) in isolated working mouse hearts during normoxic conditions (control group) and following a 15 min global no-flow ischemic period (reperfusion group). Fifty min following reperfusion (10 min Langendorff perfusion + 40 min working heart perfusion) aortic flow, coronary flow, cardiac output, peak systolic pressure and heart rate were 44 ± 4, 88 ± 4, 57 ± 4, 94 ± 2 and 81 ± 4% of pre-ischemic values). Rates of glycolysis and glucose oxidation in the reperfusion group (13.6 ± 0.8 and 2.8 ± 0.2 μmol/min/g dry wt) were not different from the control group (12.3 ± 0.6 and 2.5 ± 0.2 umol/min/g dry wt). Palmitate oxidation, however, was markedly elevated in the reperfusion group as compared to the control group (576 ± 37 vs. 357 ±21 nmol/min/g dry wt, p < 0.05). This change in myocardial substrate utilization was accompanied by a marked fall in cardiac efficiency measured as cardiac output/oxidative ATP production (136 ± 10 vs. 54 ± 5 ml/μmol ATP, p < 0.05, control and reperfusion group, respectively). We conclude that ischemia-reperfusion in isolated working mouse hearts is associated with a shift in myocardial substrate utilization in favour of fatty acids, in line with previous observations in rat. (Mol Cell Biochem 249: 97–103, 2003)

Key words

working mouse heart preparation cardiac metabolism palmitate oxidation glucose oxidation glycolysis ischemia-reperfusion 

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Copyright information

© Springer Science+Business Media New York  2003

Authors and Affiliations

  • Ellen Aasum
    • 1
  • Anne D. Hafstad
    • 1
  • Terje S. Larsen
    • 1
  1. 1.Department of Medical PhysiologyInstitute of Medical Biology, Faculty of Medicine, University of TromsøTromsøNorway

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