Abstract
Recently, increasing attention is being paid to the viral etiology of atherosclerosis (AS). Human cytomegalovirus (HCMV) is thought to be the most possible etiological factor of AS. In our study, artery vascular tissue specimens derived from 75 patients with AS were studied for detection of HCMV immediately early (IE) and later (L) gene fragments by polymerase chain reaction (PCR) and in situ hybridization; sera collected from the same patients were examined for HCMV specific IgG and IgM by enzyme-linked immunosorbent assay (ELISA). Twenty two normal arterial tissues and sera were used as controls. We found that the positive rate of HCMV L and IE gene fragments were significantly higher in AS patients than those in controls. HCMV DNA were mainly observed in nucleus of endothelial cells and muscularis under intima as well as smooth muscle of media of AS area, rarely found in controls. These results suggested that HCMV infection may relate to the pathogenesis of AS; and the artery itself may be the site of HCMV latency. In addition, higher levels of HCMV IgG and IgM were found to be associated with virus persistence, indicating that a periodically active latent infection or a continuously active infection is presented in AS patients. (Mol Cell Biochem 249: 91–96, 2003)
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References
Castelli W: Epidemiology of coronary artery disease. Am J Med 76: 4–12, 1984
Martinez A, Castro A, Gil C et al.: Recent strategies in the development of new human cytomegalovirus inhibitors. Med Res Rev 21: 227–244, 2001
Fish KN, Stenglein SG, Ibanez C, Nelson JA: Cytomegalovirus persistence in macrophages and endothelial cells. Scand J Infect Dis 99(suppl): 34–40, 1995
Sommerschild HT: Is infection a pathogenetic factor in coronary heart disease? Ugeskr Laeger 163: 1834–1842, 2001
White SJ, Nicklin SA, Sawamura T, Baker AH: Identification of peptides that target the endothelial cell-specific LOX-1 receptor. Hypertension 37: 449–455, 2001
Legrand A, Mayar EP, Dalvi SS, Nachtigal M: Transformation of rabbit vascular smooth muscle cells by human cytomegalovirus morphological transforming region I. Am J Pathol 151: 1387–1395, 1997
Zhou YF, Guetta E, Yu ZX, Finkel T, Epstein SE: Human cytomegalovirus increases modified low density lipoprotein uptake and scavenger receptor mRNA expression in vascular smooth muscle cells. J Clin Invest 98: 2129–2138, 1996
Hendrix MG, Dormans PH, Kitslaar P, Bosman F, Bruggeman CA: The presence of cytomegalovirus nucleic acids in arterial walls of atherosclerotic and non-atherosclerotic patients. Am J Pathol 134:1151–1157, 1989
Haraszthy VI, Zambon JJ, Trevisan M, Zeid M, Genco RJ: Identification of periodontal pathogens in atheromatous plaques. J Periodontol 71: 1554–1560, 2000
Castillo JP, Yurochko AD, Kowalik T: Role of human cytomegalovirus immediate-early proteins in cell growth control. J Virol 74: 8028–8037, 2000
Hsich E, Zhou YF, Paigen B, Johnson TM, Burnett MS, Epstein SE: Cytomegalovirus infection increases development of atherosclerosis in Apolipoprotein-E knockout mice. Atherosclerosis 156: 23–28, 2001
Grattan MT, Moreno-Cabral CE, Starnes VA, Oyer PE, Stinson EB, Shumway NE: Cytomegalovirus infection is associated with cardiac allograft rejection and atherosclerosis. JAMA 261: 3561–3566, 1989
Tanaka K, Zou JP, Takeda K, Ferrans VJ, Sandford GR, Johnson TM, Finkel T, Epstein SE: Effects of human cytomegalovirus immediateearly proteins on p53-mediated apoptosis in coronary artery smooth muscle cells. Circulation 99: 1656–1659, 1999
Speir E, Modali R, Huang ES, Leon MB, Shawl F, Finkel T, Epstein SE: Potential role of human cytomegalovirus and P53 interaction in coronary restenosis. Science 265: 391–394, 1994
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Chen, R., Xiong, S., Yang, Y., Fu, W., Wang, Y., Ge, J. (2003). The relationship between human cytomegalovirus infection and atherosclerosis development. In: Gilchrist, J.S.C., Tappia, P.S., Netticadan, T. (eds) Biochemistry of Diabetes and Atherosclerosis. Developments in Molecular and Cellular Biochemistry, vol 42. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9236-9_12
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DOI: https://doi.org/10.1007/978-1-4419-9236-9_12
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