Firm Adhesion of Neutrophils to Cerebral Vascular Endothelium “In Vivo”: A Role for Cysleukotrienes

  • Chiara Carnini
  • Gabriela Constantin
  • Barbara Rossi
  • Marco De Curtis
  • Giancarlo Folco
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 525)

Abstract

Interactions between neutrophils (PMNL) and endothelial cells (EC) are involved in pathological alterations of cerebral blood vessel functions, leading to circulatory disturbances such as stroke [1]. The adhesive process induces PMNL activation, coupled to local bioavailability of oxygen free radicals, proteases, cytokines and arachidonate metabolites of the 5- lipoxygenase pathway, such as leukotriene A4 and B4 (LTA4, LTB4). This contributes to alterations of vasomotor reactivity, occlusion of microvasculature and induction of vascular permeability. The ensuing PMNL emigration through the blood brain barrier represents a critical event in the initiation of cerebral inflammation. Therefore it is clear that the PMNL-EC interaction may be a target for therapeutic interventions. Significant reductions in volume of the lesions and improved physiological functions were found in rats subjected to transient MCA occlusion and treated with anti-ICAM antibody, as well as with selective PMNL depletion in a murine model of reperfused stroke.

Keywords

Permeability Prostaglandin LTB4 Cysteinyl 

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Chiara Carnini
    • 1
  • Gabriela Constantin
    • 2
  • Barbara Rossi
    • 2
  • Marco De Curtis
    • 3
  • Giancarlo Folco
    • 1
  1. 1.Department of Pharmacological SciencesUniversity of MilanMilanItaly
  2. 2.Department of PathologyUniversity of VeronaVeronaItaly
  3. 3.Istituto Neurologico BestaMilanItaly

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