Covalent Binding of Leukotriene A4 to Nucleosides, Nucleotides, and Nucleic Acids
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The chemical reactivity of leukotriene A4 (LTA4) is widely appreciated following the detailed studies of its stability in aqueous solutions, solutions containing organic solvents, and solutions containing albumin . The half-life of LTA4 is less than one second unless a stabilizing substance is present in aqueous buffers. This has led to the concept that intracellular LTA4 exists only for a brief period of time before it is either nonenzymatically hydrolyzed to 6-trans-LTB4 isomers or converted by LTA4 hydrolase or LTC4 synthase into the biologically active leukotrienes, LTB4 and LTC4, respectively []. The reactivity of LTA4 is due to its electrophilic character and within the cell various nucleophiles are present including nucleic acids which could, in theory, donate electrons into the conjugated triene epoxide moiety of LTA4.
KeywordsCovalent Binding Calcium Ionophore Calcium Ionophore A23187 Electrophilic Character LTA4 Hydrolase
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