Advertisement

The Leukotriene Receptor CYSLT1 And 5- Lipoxygenase Are Upregulated In Colon Cancer

  • Christian K. Nielsen
  • John F Öhd
  • Katarina Wikström
  • Ramin Massoumi
  • Sailaja Paruchuri
  • Maria Juhas
  • Anita Sjölander
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 525)

Abstract

The metabolites of arachidonic acid are well connected to pathological situations such as inflammation, cancer and asthmA. Sheng et al. [7] found that COX-2 is upregulated in colon cancer tissue and tumor cell lines indicating that COX-2 is involved in colon cancer. This is supported by studies showing that patients treated with nonsteroidal anti-inflammatory drugs, inhibitors of COX-2, exhibit a lower frequency of colon cancer [8]. When the non-transformed intestinal epithelial cell line, Int 407 was stimulated with LTD4 or LTB4 we observed an accumulation of COX-2 in membrane fractions as well as an increased production of prostaglandin E2 [5]. Treatment of these cells with the COX-2 inhibitor NS-398 caused apoptosis and this effect could be prevented by LTD4 [5] or LTB4 [4]. Similar results were obtained when cell viability with LTD4 or LTB4 in the presence or absence of NS-398 was assayed [4,5]. The results demonstrate that these leukotrienes can suppress the NS-398 induced apoptosis in intestinal cells.

Keywords

Colon Cancer Colon Cancer Cell Membrane Fraction Intestinal Epithelial Cell Colorectal Adenocarcinoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Krajewska M, Moss SF, Krajewski S, Song K, Holt PR, Reed JC. Elevated expression of BcL-X and reduced Bak in primary colorectal adenocarcinomas. Cancer Res 1996; 56: 2422–2427.PubMedGoogle Scholar
  2. 2.
    Massoumi R, and Sjölander A. Leukotriene D4 affects localisation of vinculin in intestinal epithelial cells via distinct tyrosine kinase and protein kinase C controlled events. J Cell Sci 2001; 114: 1925–1934.PubMedGoogle Scholar
  3. 3.
    Öhd JF, Nielsen CK, Campbell J, Landberg G, Löfberg H, and Sjölander A. Expression of the leukotriene D4 receptor CysLT1, COX-2, and other cell survival factors in colorectal adenocarcinomas. Gastroenterology 2003; 124: 57–70.PubMedCrossRefGoogle Scholar
  4. 4.
    Öhd JF, Wikström K, and Sjölander A. Do leukotrienes increase cell viability in human intestinal epithelial cells? Adv Exp Med Biol; In pressGoogle Scholar
  5. 5.
    Öhd JF, Wikström K, and Sjölander A. Leukotrienes induce cell-survival signaling in intestinal epithelial cells. Gastroenterology 2000; 119: 1007–1018.PubMedCrossRefGoogle Scholar
  6. 6.
    Paruchuri S, Hallberg B, Juhas M, Larsson C, and Sjölander A. Leukotriene D4 activates MAPK through a Ras-independent but PKCε-dependent pathway in intestinal epithelial cells. J Cell Sci 2002; 115: 1883–1893.PubMedGoogle Scholar
  7. 7.
    Sheng H, Shao J, Kirkland SC, Isakson P, Coffrey RJ, Morrow J, Beauchamp RD, and DuBois RN. Inhibition of human colon cancer cell growth by selective inhibition of cyclooxygenase-2. J Clin Inv 1997; 99: 2254–2259.CrossRefGoogle Scholar
  8. 8.
    Smalley WE, and DuBois RN. Colorectal cancer and nonsteroidal anti-inflammatory drugs. Adv Pharmacol; 39: 1–20.Google Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Christian K. Nielsen
    • 1
  • John F Öhd
    • 1
  • Katarina Wikström
    • 1
  • Ramin Massoumi
    • 1
  • Sailaja Paruchuri
    • 1
  • Maria Juhas
    • 1
  • Anita Sjölander
    • 1
  1. 1.Division of Experimental Pathology, Department of Laboratory MedicineLund University, Malmö University HospitalMalmöSweden

Personalised recommendations