Induction of Cyclooxygenase-2 Expression by Fluid Shear Stress in Vascular Endothelial Cells

  • Hiroyasu Inoue
  • Yoji Taba
  • Yoshikazu Miwa
  • Chiaki Yokota
  • Megumi Miyagi
  • Toshiyuki Sasaguri
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 525)


Vascular endothelial cells are exposed to a wide variety of biomechanical stimuli including fluid shear stress caused by blood flow. Shear stress modulates several endothelial functions, such as control of vascular tone, maintenance of antithrombotic surfaces, regulation of inflammation, protection against oxidative stresses, and regulation of endothelial cell proliferation and apoptosis.


Shear Stress Vascular Endothelial Cell Fluid Shear Stress Laminar Shear Stress mRNA Instability Motif 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Topper JN, Cai J, Falb D, Gimbrone MA Jr. Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli. Proc Natl Acad Sci USA 1996; 93:10417–10422.PubMedCrossRefGoogle Scholar
  2. 2.
    Okahara K, Sun B, Kambayashi J. Arterioscler Thromb Vasc Biol Upregulation of prostacyclin synthesis-related gene expression by shear stress in vascular endothelial cells. 1998; 18:1922–1926.Google Scholar
  3. 3.
    Ogasawara A, Arakawa, T, Kaneda T, Takuma T, Sato T, Kaneko H, Kumegawa M, Hakeda Y. Fluid shear stress-induced cyclooxygenase-2 expression is mediated by C/EBPβ, cAMP-response element-binding protein, and AP-1 in osteoblastic MC3T3-El cells. J Biol Chem 2001; 276:7048–7054.PubMedCrossRefGoogle Scholar
  4. 4.
    Inoue H, Umesono K, Nishimori T, Hirata Y, Tanabe T. Glucocorticoid-mediated suppression of the promoter activity of the cyclooxygenase-2 gene is modulated by expression of its receptor in vascular endothelial cells. Biochem Biophys Res Commun 1999; 254:292–298.PubMedCrossRefGoogle Scholar
  5. 5.
    Taba Y, Sasaguri T, Miyagi M, Abumiya T, Miwa Y, Ikeda T, Mitsumata M. Fluid shear stress induces lipocalin-type prostaglandin D2 synthase expression in vascular endothelial cells. Circ Res 2000; 86:967–973.PubMedCrossRefGoogle Scholar
  6. 6.
    Inoue H, Tanabe T, Umesono K. Feedback control of cyclooxygenase-2 expression through PPARy. J Biol Chem. 2000; 275: 28028–28032.PubMedGoogle Scholar
  7. 7.
    Inoue H, Taba Y, Miwa Y, Yokota C, Miyagi M, Sasaguri T. Transcriptional and posttranscriptional regulation of cyclooxygenase-2 expression by fluid shear stress in vascular endothelial cells. Arterioscler Thromb Vasc Biol 2002; 22:1415–1420.PubMedCrossRefGoogle Scholar
  8. 8.
    McAdam BF, Catella-Lawson F, Mardini IA, Kapoor S, Lawson JA, FitzGerald GA. Systemic biosynthesis of prostacyclin by cyclooxygenase (COX)-2. Proc Natl Acad Sci USA 1999;96: 272–277.PubMedCrossRefGoogle Scholar
  9. 9.
    Sebaldt RJ, Sheller JR, Oates, JA, Roberts II LJ, FitzGerald GA. Inhibition of eicosanoid biosynthesis by glucocorticoids in humans. Proc Natl Acad Sci USA 1990; 87:6974–6978PubMedCrossRefGoogle Scholar
  10. 10.
    Ueno N, Murakami M, Tanioka T, Fujimori K, Tanabe T, Urade Y, Kudo I. Coupling between cyclooxygenase, terminal prostanoid synthase, and phospholipase A2. J Biol Chem 2001; 276:34918–34927.PubMedCrossRefGoogle Scholar
  11. 11.
    Miwa Y, Sasaguri T, Inoue H, Taba Y, Ishida A, Abumiya T. 15-deoxy-α12,14-prostaglandin J2 induces G1 arrest and differentiation marker expression in vascular smooth muscle cells. Mol Pharmacol 2000; 58:837–844.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Hiroyasu Inoue
    • 1
  • Yoji Taba
    • 2
  • Yoshikazu Miwa
    • 2
  • Chiaki Yokota
    • 3
  • Megumi Miyagi
    • 2
  • Toshiyuki Sasaguri
    • 2
  1. 1.Department of PharmacologyNational Cardiovascular, Center Research InstituteOsakaJapan
  2. 2.Department of Clinical Pharmacology, Graduate School of Medical SciencesKyushu UniversityFukuokaJapan
  3. 3.Cerebrovascular LaboratoryNational Cardiovascular, Center Research InstituteOsakaJapan

Personalised recommendations