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5-Lipoxygenase Activation by Mapkapk-2 and Erks

  • Oliver Werz
  • Eva Bürkert
  • Lutz Fischer
  • Dagmar Szellas
  • David Dishart
  • Bengt Samuelsson
  • Olof Rådmark
  • Dieter Steinhilber
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 525)

Abstract

5-Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of proinflammatory leukotrienes from arachidonic acid (AA) [1]. Ca2+, phosphatidylcholine, ATP, and hydroperoxides stimulate the enzymatic activity of 5-LO in vitro [1]. However, the mechanisms involved in the agonist-induced 5-LO activation in intact cells are less clear. Stimuli that cause an elevation of the intracellular Ca2+ levels activate 5-LO. Ca2+ binds 5-LO in vitro at the enzyme’s C2 domain [2], which is a prerequisite for association with membranes [3].

Keywords

Arachidonic Acid Free Arachidonic Acid MAPK Inhibitor SB203580 Efficient Phosphorylation Exogenous Arachidonic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Oliver Werz
    • 1
  • Eva Bürkert
    • 1
  • Lutz Fischer
    • 1
  • Dagmar Szellas
    • 1
  • David Dishart
    • 2
  • Bengt Samuelsson
    • 2
  • Olof Rådmark
    • 2
  • Dieter Steinhilber
    • 1
  1. 1.Institute of Pharmaceutical ChemistryUniversity of FrankfurtFrankfurtGermany
  2. 2.Division of Physiological Chemistry IIDepartment of Medical Biochemistry and BiophysicsStockholmSweden

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