Abstract
Prostaglandin E synthases (PGES) comprise a number of structurally different enzymes that convert the product of the cyclooxygenase enzymes, PGH2, into the physiologically important PGE2. An inducible microsomal PGES (mPGES-1) couples preferentially with COX-2 [1], whereas a constitutively and widely expressed cytosolic PGES (cPGES or p23) is believed to couple with Cox-1 [2]. Both of these enzyme activities are dependent on GSH. Most recently, a second, membrane-associated PGES (mPGES-2) that has a wide tissue distribution and is activated by a number of thio1-containing reagents was identified and purified [3]. Two cytosolic glutathione transferases (μ2 and μ3) also have some PGES activity. As the expression of mPGES-1 is strongly induced by pro-inflammatory stimuli and is down-regulated by glucocorticoids, mPGES-1 is a potential drug target for anti-inflammatory therapy. A solubilization and partial purification of a microsomal PGES activity from bovine vesicular glands has been reported [4]. It appears that this enzyme is the same as mPGES-1. Here we report the baculovirus over-expression of recombinant human mPGES-1 in Sf9 insect cells, its purification to apparent homogeneity and the characterization of its enzymatic properties.
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References
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Ouellet, M. et al. (2003). Expression, Purification and Characterization of Recombinant Human Microsomal PGE2 Synthase-1. In: Yazici, Z., Folco, G.C., Drazen, J.M., Nigam, S., Shimizu, T. (eds) Advances in Prostaglandin, Leukotriene, and other Bioactive Lipid Research. Advances in Experimental Medicine and Biology, vol 525. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9194-2_22
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DOI: https://doi.org/10.1007/978-1-4419-9194-2_22
Publisher Name: Springer, Boston, MA
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