Abstract
Pseudomonas putida strains are rapidly growing bacteria, frequently isolated from most temperate soils and waters, particularly polluted soils. They are nutritional opportunists par excellence and a paradigm of metabolically versatile microorganisms that recycle organic wastes in aerobic and microaerophilic compartments of the environment, and that plays a key role in the maintenance of environmental quality. P putida strain KT24402, 53 is probably the best-characterized saprophytic laboratory Pseudomonad that has retained its ability to survive and function in the environment. The bacterium is a plasmid-free derivative of a toluene-degrading bacterium, originally designated Pseudomonas arvilla strain mt-246 and subsequently reclassified as P putida mt-243, 68. It is the first Gram-negative soil bacterium to be certified by the Recombinant DNA Advisory Committee (RAC) of the United States National Institutes of Health as the host strain of a host-vector biosafety (HV1) system for gene cloning in Gram-negative soil bacteria21. An extensive spectrum of versatile genetic tools, in particular mini-transposons and tools based on these, have been developed for its analysis, manipulation and use as a host for cloned genes from other soil organisms 12, 13, 35, 41,. KT2440 is being exploited in the development of a variety of biotechnological applications, including the design of new catabolic pathways for pollutants 19, 51, 56, the production by biocatalysis of intermediates, including chiral synthons for chemical syntheses72, and quality improvement of fossil fuels, for example by desulphurization24. KT2440 is alsi able to colonize the rhizosphere of a variety of crop plants, such as corn plants, wheat, strawberry, sugarcane and spinach 20, and is being used to develop new biopesticides and plant growth promoters that function in the plant rhizosphere.
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Martins dos Santos, V.A.P., Timmis, K.N., Tümmler, B., Weinel, C. (2004). Genomic Features of Pseudomonas putida Strain KT2440. In: Ramos, JL. (eds) Pseudomonas. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9086-0_3
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