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Molecular Tools for Genetic Analysis of Pseudomonads

  • Chapter
Pseudomonas

Abstract

The genomes of several pseudomonads, including Pseudomonas aeruginosa 65, P. putida 110, P. syringae (http://www.tigr.org), and P. fluorescens (http://www.sanger.ac.uk), have been entirely sequenced. To take advantage of the wealth of information revealed by these sequences and especially to reveal the role of the many orphan genes contained in these genomes, versatile tools are needed to gain access to their biological functions. Such tools include cloning and expression plasmids, rapid methods for cloning of large DNA segments, allele replacement vectors, tools for development of host strains for cloning and expression vectors, reporter genes for gene regulation studies, and vectors for integration of extraneous DNA (including reporter genes, regulatory elements, biosensors, etc.) into the chromosome. The growing availability of genomes facilitates genome-wide gene identification projects using insertion mutagenesis, genetic surveys of gene families involved in complex phenotypes and comparative genome analyses of different strains. Although the arsenal of genetic tools available for genetic analysis of pseudomonads has steadily grown and improved over the last decade, many of them still need improvement to complement other modem tools available for genomic analysis, including microarray and proteomic analyses.

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  1. Department of Microbiology Immunology and Pathology, Colorado State University, Fort Collins, CO, 80523-1682, USA

    Herbert P. Schweizer

  2. Centro Nacional de Biotecnología del Consejo Superior de Investigaciones Científicas (CSIC), Campus de Cantoblanco, 28049, Madrid, Spain

    Víctor de Lorenzo

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  1. Herbert P. Schweizer
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  1. CSIC, Granada, Spain

    Juan-Luis Ramos

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Schweizer, H.P., de Lorenzo, V. (2004). Molecular Tools for Genetic Analysis of Pseudomonads. In: Ramos, JL. (eds) Pseudomonas. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9086-0_10

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