Abstract
Analysis of saturated fatty acid (FA) in cultured fibroblasts is used for the diagnosis of X-linked Adrenoleukodystrophy (X-ALD). The culture mediums normally used are basic mediums such as Hank’s, Eagle’s or RPMI plus calf serum. Due to a variable expression of ALDP (the defective protein in X-ALD) observed in cells cultured in different mediums (Ruiz et al 1997), we have studied the influence of the culture medium in the biochemical parameters used in X-ALD diagnosis. We have focused our attention in FA profile.
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References
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Giros, M., Ruiz, M. (2003). Modifying the Peroxisomes by Cell & Tissue Culture. In: Roels, F., Baes, M., De Bie, S. (eds) Peroxisomal Disorders and Regulation of Genes. Advances in Experimental Medicine and Biology, vol 544. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9072-3_34
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DOI: https://doi.org/10.1007/978-1-4419-9072-3_34
Publisher Name: Springer, Boston, MA
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