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RNA Interference in Mammalian Systems - A Practical Approach

  • Conference paper
Peroxisomal Disorders and Regulation of Genes

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 544))

Abstract

RNAi-related mechanisms are evolutionarily conserved and are a tool for cells to defend against foreign nucleic acid invasion and thus genomic instability in the cell. Double-stranded RNA is relatively easy to produce in vitro and, its introduction generates knockout phenotypes without the need to interfere at the DNA level. Thus, the discovery of RNAi has greatly contributed to research by providing a new genetic tool.

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Authors and Affiliations

  1. Babraham Bioincubators, Polgen Cyclacel, Ltd, BabrahamCB2 4AT, UK, MZG is at Wellcome CR UK institute, Cambridge, UK

    Joanna B. Grabarek & Magdalena Zernicka-Goetz

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  1. Joanna B. Grabarek
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  2. Magdalena Zernicka-Goetz
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Editors and Affiliations

  1. Ghent University, Ghent, Belgium

    Frank Roels  & Sylvia De Bie  & 

  2. K.U. Leuven, Leuven, Belgium

    Myriam Baes

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© 2003 Springer Science+Business Media New York

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Grabarek, J.B., Zernicka-Goetz, M. (2003). RNA Interference in Mammalian Systems - A Practical Approach. In: Roels, F., Baes, M., De Bie, S. (eds) Peroxisomal Disorders and Regulation of Genes. Advances in Experimental Medicine and Biology, vol 544. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9072-3_24

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  • DOI: https://doi.org/10.1007/978-1-4419-9072-3_24

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4782-8

  • Online ISBN: 978-1-4419-9072-3

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