Genes Regulating Cardiovascular Function as Revealed Using Viral Vectors
This chapter illustrates briefly the usefulness of viral vectors in studying genes regulating the cardiovascular system in both health and disease. Some advantages of using viral vectors are discussed and recent examples illustrated. A discussion follows on possible refinements for viral vectors as tools for systems physiology. This includes the use of cell-specific promoters, longer term expression with lenti virus and ’vigilent’ vectors.
Keywordsadenovirus hypertension baroreceptor reflex rat somatic gene transfer
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- Coleman, J.E., Huentelman, M, Kasparov, S., Metcalfe, B.L., Paton, J.F.R., Katovich, M.J., Semple-Rowland, S.L., Raizada, M.K., 2003. Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Functional Genomics 12221–228.Google Scholar
- Kureishi, Y., Luo, Z., Shiojima, I., Bialik, A., Fulton, D., Lefer, D.J., Sessa, W.C., Walsh, K., 2000. The HMG-CoA reductase inhibitor simvastatin activates the protein kinase Akt and promotes angiogenesis in normocholesterolemic animals. Nature Med. 9,1004–1010.Google Scholar
- Paton J.F.R., Deuchars, J., Ahmad, Z., Wong, L.-F., Murphy, D., Kasparov, S., 2001. Adeno viral vector demonstrates that angiotensin II induced depression of the cardiac baroreflex is mediated by endothelial nitric oxide synthase in the nucleus tractus solitarii. J. Physiol. 531.2445–458.CrossRefGoogle Scholar