Advertisement

Genes Regulating Cardiovascular Function as Revealed Using Viral Vectors

  • Julian F.R. Paton
  • Hidefumi Waki
  • Mohan Raizada
  • Sergey Kasparov

Abstract

This chapter illustrates briefly the usefulness of viral vectors in studying genes regulating the cardiovascular system in both health and disease. Some advantages of using viral vectors are discussed and recent examples illustrated. A discussion follows on possible refinements for viral vectors as tools for systems physiology. This includes the use of cell-specific promoters, longer term expression with lenti virus and ’vigilent’ vectors.

Keywords

adenovirus hypertension baroreceptor reflex rat somatic gene transfer 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brenner, S., 1994. Loose ends. Current Biology 4864.CrossRefGoogle Scholar
  2. Coleman, J.E., Huentelman, M, Kasparov, S., Metcalfe, B.L., Paton, J.F.R., Katovich, M.J., Semple-Rowland, S.L., Raizada, M.K., 2003. Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Functional Genomics 12221–228.Google Scholar
  3. Datta, S.R., Brunet, A., Greenberg, M.E., 1999. Cellular survival: a play in three Akts. Genes and Development 132905–2927.PubMedCrossRefGoogle Scholar
  4. Geddes B.J., Harding, T.C., Lightman S.L., Uney J.B. (1999). Assessing viral gene therapy in neuroendocrine models. Frontiers in Neuroendocrinology 20296–316.PubMedCrossRefGoogle Scholar
  5. Harding, T.C., Geddes, B.J., Murphy, D., Knight, D., Uney, J.B., 1998. Switching transgene expression in the brain using an adenoviral tetracyclineregulatable system. Nature Biotechnology 16553–555.PubMedCrossRefGoogle Scholar
  6. Kantor, D. B., Lanzrein, M., Stary, S. J., Sandoval, G M., Smith, W. B., Sullivan, B. M., Davidson, N., Schuman, E.M., 1996. A role for endothelial NO synthase in LTP revealed by adenovirus-mediated inhibition and rescue. Science 2741744–1748.PubMedCrossRefGoogle Scholar
  7. Kishi, T. Hirooka, Y, Kimura, Y., Sakai, K., Ito, K., Shimokawa, H., Takeshita, A., 2003. Overexpression of eNOS in RVLM improves impaired baroreflex control of heart rate in SHRSP. Hypertension 41255–260PubMedCrossRefGoogle Scholar
  8. Kureishi, Y., Luo, Z., Shiojima, I., Bialik, A., Fulton, D., Lefer, D.J., Sessa, W.C., Walsh, K., 2000. The HMG-CoA reductase inhibitor simvastatin activates the protein kinase Akt and promotes angiogenesis in normocholesterolemic animals. Nature Med. 9,1004–1010.Google Scholar
  9. Luo, Z., Fujio, Y., Kureishi, Y., Rudic, R.D., Daumerie, G., Fulton, D., Sessa, W.C., Walsh, K., 2000. Acute modulation of endothelial Akt/PKB activity alters nitric oxide-dependent vasomotor activity in vivo. J. Clin. Inv. 106439–499.CrossRefGoogle Scholar
  10. Miao, W., Luo, Z., Kitsis, R.N., Walsh, K., 2000. Intracoronary, adenovirus-mediated Akt gene transfer in heart limits infarct size following ischaemia-reperfusion injury in vivo. J. Molec. Cell. Cardio. 322397–2402.CrossRefGoogle Scholar
  11. Paton J.F.R., Deuchars, J., Ahmad, Z., Wong, L.-F., Murphy, D., Kasparov, S., 2001. Adeno viral vector demonstrates that angiotensin II induced depression of the cardiac baroreflex is mediated by endothelial nitric oxide synthase in the nucleus tractus solitarii. J. Physiol. 531.2445–458.CrossRefGoogle Scholar
  12. Paton, J.F.R., Kasparov, S., Paterson, D.J., 2002. Site-Specific Differential Modulation of Cardiac Autonomic Control by Nitric Oxide. TINS 25626–631.PubMedGoogle Scholar
  13. Phillips, M.I., Tang, Y., Schmidt-Ott, K., Quian, K., Kagiyama, S., 2002. Vigilent vector: Heart specific promoter in an adeno associated virus vector for cardioprotection. Hypertension 39651–655.PubMedCrossRefGoogle Scholar
  14. Waki, H., Kasparov, S., Wong, L.-F., Murphy, D., Shimizu, T., Paton, J.F.R., 2003. Chronic inhibition of eNOS activity in NTS enhances baroreceptor reflex in conscious rats. J. Physiol. 546.1,233–242.CrossRefGoogle Scholar
  15. Wong, L.-F. Poison, J., Murphy D., Paton, J.F.R., Kasparov, S., 2002. Molecular and pharmacological dissection of the intracellular pathways mediating the angiotensin II induced baroreceptor reflex depression in the nucleus of the solitary tract. FASEB J. 161595–1601.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2004

Authors and Affiliations

  • Julian F.R. Paton
    • 1
  • Hidefumi Waki
    • 1
  • Mohan Raizada
    • 2
  • Sergey Kasparov
    • 1
  1. 1.Department of Physiology, School of Medical SciencesUniversity of BristolBristolUK
  2. 2.Department of PhysiologyUniversity of Florida, College of MedicineGainesvilleUSA

Personalised recommendations