Abstract
In order to study the mechanical activity of a single cardiac myocyte under a wide range of load, we have developed a novel force measurement system using carbon fibers. Newly fabricated Graphite Reinforced by Carbon (GRC) fibers greatly facilitate the firm attachment of cell membrane to the fibers. A pair of fibers was attached to both ends of the cell; the rigid fiber as a mechanical ground and the compliant fiber for the strain gauge. By connecting the compliant fiber to the piezoelectric translator and applying the position signal to the driver, we could make the myocyte contract under isometric condition. Feedback control of the system also enabled us to study the relation between work output and the load. This system can be a useful tool in studying the mechanical activity of the cardiac myocyte under genetic as well as pharmacological interventions.
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References
A.J. Brady. Mechanical properties of isolated cardiac myocytes. Phyiol. Rev. 71:413–428 (1991).
A. Fabiato. Myoplasmic free calcium concentration reached during the twitch of an intact isolated cardiac cell and during calcium-induced release of calcium from the sarcoplasmic reticulum of a skinned cardiac cell from the adult rat or rabbit ventricle. J Gen Physiol. 78(5):457–497 (1981).
D. Gamier. Attachment procedures for mechanical manipulation of isolated cardiac myocytes: a challenge. Cardiovasc Res. 28:1958–1964 (1994).
S.I. Yasuda, S. Sugiura, N. Kobayakawa, H. Fujita, H. Yamashita, K. Katoh, et al. A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers. Am J Physiol Heart Circ Physiol. 281(3):H1442–6(2001).
M. Yoneda. Force exerted by a single cilium of Mytilus Edulus. I. Exo Biol. 37:461–468 (1960).
S. Kamimura. Direct measurement of nanometric displacement under an optical microscope. Applied Optics. 26:3425–3427(1987).
C. Tasche, E. Meyhofer, B. Brenner. A force transducer for measuring mechanical properties of single cardiac myocytes. Am J Physiol. 277(6 Pt 2):H2400–8 (1999).
H. Asanoi, S. Sasayama, T. Kameyama. Ventriculoarterial coupling in normal and failing heart in humans [published erratum appears in Circ Res 1990Apr;66(4):1170]. Circ Res. 65(2):483–493 (1989).
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Sugiura, S. et al. (2003). Measurement of Force Developed by a Single Cardiac Myocyte Using Novel Carbon Fibers. In: Sugi, H. (eds) Molecular and Cellular Aspects of Muscle Contraction. Advances in Experimental Medicine and Biology, vol 538. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9029-7_35
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DOI: https://doi.org/10.1007/978-1-4419-9029-7_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4764-4
Online ISBN: 978-1-4419-9029-7
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