Abstract
One of the persistent mysteries in clinical in vitro fertilization (IVF) is why embryos that appear to be developing normally throughout the preimplantation stages fail to implant after uterine transfer. While it has been proposed that a certain proportion of failures may have a genetic or chromosomal basis, it has also been suggested that the inability of the embryo to emerge from the confines of the zona pellucida at the expanded blastocyst stage by a process commonly termed ‘hatching’ may render an otherwise normal embryo incompetent to establish a pregnancy owing to the persistence of a physical barrier between embryo and a receptive endometrial epithelium. The notion that the zona pellucida may be refractory to hatching is derived from observations of human embryos cultured in vitro through the blastocyst stages where some proportion of embryos within cohorts cultured in the same dish remain entrapped within the sibling blastocysts hatch and emerge completely. Although reasons why differential resistance to hatching may exist between embryos in the same and different cohorts is unknown, physical and biochemical differences in zona structure, zona hardening in response to culture conditions that are presumed to be suboptimal, and embryo defects which prevent an active participation in emergence have been suggested.
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References
Alikani, M., Cohen, J., Tomkin, G., et al (1999). Human embryo fragmentation in vitro and its implications for pregnancy and implantation. Fertil. Steril. 71: 836–842.
Alikani, M., Noyes, N, Cohen J., et al. (1994). Monoozygous twinning in the human is associated with the zona pellucida architecture. Hum. Reprod. 9: 1318–1321.
Alikani, M., and Cohen, J. (1992). Micromanipulation of cleaved embryos cultured in proteinfree medium: a mouse model for assisted hatching. J Exp. Zool. 2. 262: 458–463.
Balaban, B., Urman, B., Alatas,.C, et. al. (2002). A comparison of four different techniques of assisted hatching. Hum. Reprod. 17: 1239–43.
Baruffi, R., Mauri, A., Petersen, C., et. al. (2000). Zona thinning with noncontact diode laser in patients aged ≤37 years with no previous failure of implantation: a prospective randomized study. J Assist Reprod Genet. 17: 557–560.
Bauskin, A., Franken, D., Eberspaecher, U. and Donner, P. (1999). Characterization of human zona pellucida glycoproteins. Mol. Hum. Reprod. 5: 534–540.
Bertrand, E., Van Den Bergh, M. and Englert, Y. (1995). Does zona pellucida thickness influence the fertilization rate? Hum. Reprod. 10: 1189–1193.
Bertrand, E, VanDen Bergh, M., and Englert, Y. (1999). Clinical parameters influencing human zona pellucida thickness. Fertil. Steril. 66: 408–411.
Bider, D., Livshits, A., Yonish, M., et. al. (1997). Assisted hatching by zona drilling of human embryos in women of advanced age. Hum. Reprod. 12: 317–320.
Bulmer, M. (1970). The Biology of Twinning in Man. Clarendon Press, Oxford.
Check, J., Hoover, L., Nazari, A., et. al. (1996). The effect of assisted hatching on pregnancy rates after frozen embryo transfer. Fertil. Steril. 65: 254–260.
Cohen, J., Simons, R., Edwards, R., et. al. (1985). Pregnancies following the frozen storage of expanding human blastocysts. J. IVF-ET Trans. 2: 59–64.
Cohen, J., Eisner, C., Kort, H., et. al., (1990). Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum. Reprod. 5: 7–13.
Cohen, J., Inge, K., Suzman, M., et. al. (1989). Videocinematography of fresh and cryopreserved embryos: a retrospective analysis of embryonic morphology and implantation. Fertil. Steril. 51: 820–827.
Cohen, J., Malter, H., Wrigh,t G., et. al. (1989). Partial zona dissection of human oocytes when failure of zona pellucida penetration is anticipated. Hum. Reprod. 4: 435–442.
Cohen, J., Malter, H., Talansky, B. and Grifo, J. (eds). (1991). Micromanipulation of Human Oocytes and Embryos. New York: raven Press, pp: 191–222.
Cohen, J., Eisner, C., Kort, H., et. al. (1990). Immunosuppresion supports implantation of zona pellucida dissected human embryos. Fertil. Steril. 53: 662–665.
Cohen, J Alikani, M., Trowbridge, J., and Rosenwaks, Z. (1992). Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum. Reprod. 7: 685–691.
Dale, B., Gualtieri, R., Talevi, R., et. al. (1999). Intercellular communication n the early human embryo. Mol. Reprod. Dev. 29: 22–28.
De Felici, M., Salustri, A. and Siracusa, G. (1982),’ spontaneous’ hardening of the zona pellucida of mouse oocyte during in vitro culture. Gam. Res. 12: 227–235.
Derom, C., Vlietinck, R., Derom, R., et. al. (1987). Increased monozygotic twinning rate after ovulation induction. Lancet 1: 1236–1238.
Develioglu, O., Cox, B., Toner, J., et. al. (1999). The value of basal serum follicle stimulating hormone, luteinizing hormone and oestradiol concentrations following pituitary downregulation in predicting ovarian response to stimulation with highly purified follicle stimulating hormone. Hum. Reprod. 14: 1168–74.
Douglas-Hamilton, D. and Conia, J. (2001). Thermal effects in laser-assisted pre-embryo zona drilling. J Biomed Opt. 6: 205–213.
Edirisinghe, W., Ahnonkitpanit, V., Promviengchai, S., et al. (1999). A study failing to determine significant benefits from assisted hatching: patients selected for advanced age, zonal thickness of embryos, andprevious failed attempts. Clin. Assist. Reprod. 16: 294–301.
Edwards, R., Mettler, L. and Walters, D. (1986). Identical twins and in-vitro fertilization. J. IVF-ET. 3: 114–117.
Familiari, G., Nottola, S., Macchiarellim G., e.t al. (1992). Human zona pellucida during in vitro fertilization: an ultrastructural study using saponin, ruthenium red, and osmiumthiocarbohydrazide. Mol. Reprod. Dev. 32: 51–61.
Feichtinger, W., Strohmer, H., Fuhrberg, P., et. al. (1992). Photablation of oocyte zona pellucida by erbium-yag laser for in-vitro fertilisation in severe male infertility. Lancet 339: 811.
Fong, C., Bongso, A., Ng, A., et. al. (1998). Blastocyst transfer after enzymatic treatment of the zona pellucida: Improving in vitro fertilization and understanding implantation. Hum. Reprod. 13: 2926–2932.
Gordon, J. and Talansky, B. (1986). Assisted fertilization by zona drilling: a mouse model for correction of oligospermia. J. Exp. Zool. 39: 347–354.
Handyside, A., Kontogianni, E., Hardy, K. and Winston, R. (1990). Pregnancies from biopsied human preimplantation embryos sexed by Y-specific amplification. Nature 344: 378–80.
Hellebaut, S., DeSutter, P., Dozortsev, D., et. al. (1996). Does assisted hatching improve implantation rates after in vitro fertilization or intracytoplasmic sperm injection in all patients? A prospective randomized study. J. Assist. Reprod. Genet. 13: 19–22.
Hershlag, A., Paine, T., Cooper, G., e.t al. (1999). Monozygotic twinning associated with mechanical assisted hatching Fertil. Steril. 71: 144–146.
Hurst, B., Tucker, K., Awoniyi, C., and Schlaff, W. (1998), Assisted Hatching does not enhance IVF success in good prognosis patients. J. Assist. Repro. Genet. 15: 62–64.
Isik, A., Vicdan, K., Kaba, K., and Dagli, G. (2000). Comparison of zona manipulated and zona intact blastocyst transfers: A prospective randomized trial. J. Assist. Reprod. Genet. 17: 135–139.
Khalifa, E., Tucker, M., and Hunt, P. (1992). Cruciate thinning of the zona pellucida for more successful enhancement of blastocyst hatching in the mouse. Hum. Reprod. 7: 532–536.
Lanzendorf, S., Nehchiri, F. and Mayer, J. (1998). A prospective, randomized, double-blind study for the evaluation of assisted hatching in patients with advanced maternal age. Hum. Reprod. 13: 409–413.
Liu, H., Cohen, J., Alikani, M., et. al. (1993). Assisted hatching facilitates earlier implantation Fertil. Steril. 60: 871–875.
Magli, M., Gianaroli, L., Ferraretti, A., et al (1998). Rescue of implantation potential in embryos with poor prognosis by assisted zona hatching. Hum. Reprod. 5: 1331–1335.
Malter, H. and Cohen, J. (1989). Blastocyst formation and hatching in vitro following zona drilling of mouse and human embryos. Gam. Res. 24: 67–80.
Mansour, R., Rhodes, C., Aboulghar, M., et. al. (2000). Transfer of zona-free embryos improves outcome in poor prognosis patients: a prospective, randomized controlled study. Hum. Reprod. 15: 1061–64.
Mantoudis, E., Podsiadly, B., Gorgy, A. et. al. (2001). A comparison between quarter, partial and total laser assisted hatching in selected infertility patients. Hum. Reprod. 16: 2182–2186.
Meldrum, D., Wisot, A., Yee, B., et. al. (1998a). Assisted hatching reduces the age-related decline in IVF outcome in women younger than age 43 without increasing miscarriage or monozygotic twinning. Clin. Assist. Reprod. 15: 418–421.
Meldrum, D., Silverberg, K., Bustillo, M. and Stokes, L. (1998b). Success rate with repeated cycles of in vitro fertilization-embryo transfer. Fertil. Steril. 69: 1005–1009.
Mercader, A., Simon, C., Galan, A., et. al. (2001). An analysis of spontaneous hatching in a human epithelial coculture system: is assisted hatching justified? J. Assist. Reprod. Genet. 18: 315–319.
Mishra, A. and Seshagiri, P. (2000). Evidence for the involvement of a species-specific embryonic protease in zona escape of hamster blastocysts. Mol Hum Reprod. 6: 1005–1012.
Nakayama. T, Fujiwara. H., Tastumi, K., et al (1998). A new assisted hatching technique using a piezo-micromanipulator. Ferti.l Steril. 69: 784–788.
Neev, J., Gonzales, A., Licciardi F., et. al. (1993). Opening of the mouse zona pellucida by laser without a micromanipulator. Hum. Reprod. 8: 939–944.
Neev, J., Schiewe, M., Sung, V. et. al. (1995). Assisted hatching in mouse embryos using a noncontact Ho: YSGG laser system J. Assist. Reprod. Genet. 12: 288–293.
Obruca, A., Strohmer, H., Sakkas, D., et al (1994). Use of lasers in assisted fertilization and. hatching. Hum. Reprod. 9: 1723–1726.
Schieve, L., Meiklie, R., Peterson, H., et. al. (2000). Does assisted hatching pose a risk for monozygotic twinning in pregnancies conceived through in vitro fertilization? Fertil. Steril. 74: 288–294.
Schiewe, M., Hazelager, N., Scliementi, C. and Balmaceda, J. (1995). Physiological characterization of blastocyst hatching mechanisms by use of a mouse antihatching model. Fertil. Steril. 63: 288–294.
Schoolcraft, W., Schlenker, T., Gee, M., et. al. (1994). Assisted hatching in the treatment of poor prognosis in vitro fertilization candidates. Fertil. Steril. 62: 551–554.
Shiloh, H. and Dirnfeld, M. (2001). Study of zona pellucida thickness of embryos before their transfer and relation to age and follicle-stimulating hormone. Reprod. Tech. 10: 307–310.
Sills, E., Moomjy, M., Zaninovic, N., et. al. (2000). Human zona pellucida micromanipulation and monozygotic twinning frequency after IVF. Hum. Reprod. 15: 890–895.
Skupski, D., Streltzoff, J., Hutson, J., et. al. (1995). Early diagnosis of conjoined twins in triplet pregnancy after in vitro fertilization and assisted hatching. J. Ultrasound Med. 14: 611–615
Slotnick, R. and Ortega, J. (1996). Monoamniotic twinning and zona manipulation: A survey of U.S. centers correlating zona manipulation procedures and high risk twinning frequency. J Assist. Reprod. Genet. 13: 381–385.
Talevi, R., Gualtieri, R., Tartaglioni, G. and Fortunato, A. (1997). Heterogeneity of the zona pellucida carbohydrate distribution in human oocytes failing to fertilize in vitro. Hum. Reprod. 12: 2773–2780.
Tao, J. and Tamis, R. (1997). Application of assisted hatching for 2-day-old, frozen-thawed embryo transfer in a poor-prognosis population. J. Assist. Reprod. Genet. 14: 128–130.
Tucker, M., Cohen, J., Massey, J., et. al. (1991). Partial zona dissection of the zona pellucida of frozen-thawed human embryos may enhance blastocyst hatching, implantation, and pregnancy rates. Am. J. Obstet. Gynecol. 165: 341–344.
Tucker, M., Luecke, N., Wiker, S. and Wright, G. (1993). Chemical removal of the outside of the zona pellucida of day 3 human embryos has no impact on implantation rate. J. Assist. Reprod. Genet. 10: 187–191.
Van den Abbeel, E., Van der Elst, A. and Van Steirteghm, A. (1994). The effect of temperature at which slow cooling is terminated and of thawing rate on the survival of onecell mouse embryos frozen in dimethyl sulfoxide or 1,2-propanediol solutions. Cryobiology 31: 423–33.
Van Langendonckt, A., Demylle, D., Wyns, C. et al. (2001). Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study. Fertil. Steril. 76: 1023–31.
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Wright, G., Jones, A. (2004). Assisted Hatching in Clinical IVF. In: Van Blerkom, J., Gregory, L. (eds) Essential IVF. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8955-0_16
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DOI: https://doi.org/10.1007/978-1-4419-8955-0_16
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