Approaches to Devise Antibody Purification Processes by Chromatography
- 269 Downloads
Monoclonal antibodies and more largely immunoglobulins along with all their fragments and engineered forms represent today the largest class of produced and purified protein in number and quantity. New potential applications of antibodies in their native form or various engineered constructs actually continue to stimulate therapeutic areas. Expected high dosage levels imply that their purity has to meet stringent requirements. It means also that the production cost per dose compared to other therapeutic biomolecules has to be kept low. To achieve these goals it is anticipated that effective, low cost productions and purification strategies will be put in place.
KeywordsCell Culture Supernatant Cheese Whey Synthetic Ligand Affinity Sorbent Antibody Purification
Unable to display preview. Download preview PDF.
- Arguelles M.E., Alonso M., Garcia-Suarez M.D., Barneo L., Sampedro A., de los Toyos J.R. Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum-and protein-free culture supematants. Biomed. Chromatogr. 1999; 13: 379–81.PubMedCrossRefGoogle Scholar
- Boschetti E., Jungbauer A. “Separation of antibodies by liquid chromatography.” In: Handbook of Biosepartions Vol 2, S. Ahuja ed. Acad. Press, 2000, pp 535–632.Google Scholar
- Boschetti E., Guerrier L. Purification of antibodies by HCIC and impact of ligand structure. I. J. BioChromatogr. 2001b; 6: 269–83.Google Scholar
- Boschetti E., Girot P. “Ion exchange interaction biochromatography.” In: Biochromatography, Theory and Practice. M.A. Vijiayalakshmi ed. Taylor and Francis publisher., 2002a, pp. 24–45.Google Scholar
- Carlson J., lanson J. C, Sparrman M. « Affinity Chromatography.” In: Protein purification: principles, high resolution methods, and applications. A John Wiley & sons, Inc., New York. 1988.Google Scholar
- Duffy S., Moellering B. J., Prior G. M., Doyle K. R., Prior C. P. Recovery of Therapeutic-grade of antibodies: Protein A and ion exchange chromatography. BioPharm. 1989; June: 35–47.Google Scholar
- Jiskoot W, Van Hertrooij JJ, Klein Gebbinck JW, Van der Velden-de Groot T, Crommelin DJ, Beuvery EC. Two-step purification of a murine monoclonal antibody intended for therapeutic application in man. Optimisation of purification conditions and scaling up. J. Immunol. Methods. 1989; 124: 143–56.PubMedCrossRefGoogle Scholar
- Lewis J.A., Lommen D.C., Raddatz W.D., Dolan J.W., Snyder L.R., Molnar I.J. Computer simulation for the prediction of separation as a function of pH for reversed-phase high-performance liquid chromatography. I. Accuracy of a theory-based model. J. Chromatogr. 1992; 502: 183–95.Google Scholar
- Lihme A., Bendix-Hansen M. Protein A mimetic for large scale monoclonal antibody purification. Biotechnology Laboratory 1997; 15: 30–1.Google Scholar
- Mohan S. B., Lyddiatt A. “Recent developments in affinity separation technologies.” In Affinity separations. IRL Press, Oxford. 1997; ppl–38.Google Scholar
- Sharma S. K. “Designer affinity purification of recombinant proteins.” In: Affinity separations, a practical approach. Oxford University Press, Oxford. 1997, pp 197–218.Google Scholar