Methods to Study Angiogenesis in vitro
Human microvascular endothelial cells (ECs) are more difficult to isolate than macrovascular ECs. Pure cultures of human macrovascular ECs can be obtained easily from umbilical cord veins by incubation and subseqent flushing of the veins with enzymatic solutions (4). Cultures of human umbilical vein ECs (HUVECs) can be greatly expanded and usually retain markers of endothelial differentiation over more than ten passages until they eventually become senescent.
KeywordsPermeability Migration Heparin Penicillin Dimethyl
Unable to display preview. Download preview PDF.
- 8.Schmidt NO, Westphal M, Hagel C, Ergun S, Stavrou D, Rosen EM, Lamszus K: Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis. Int J Cancer 1999, 84:10-8.PubMedCrossRefGoogle Scholar