Methods to Study Angiogenesis in vitro
Human microvascular endothelial cells (ECs) are more difficult to isolate than macrovascular ECs. Pure cultures of human macrovascular ECs can be obtained easily from umbilical cord veins by incubation and subseqent flushing of the veins with enzymatic solutions (4). Cultures of human umbilical vein ECs (HUVECs) can be greatly expanded and usually retain markers of endothelial differentiation over more than ten passages until they eventually become senescent.
KeywordsVascular Endothelial Growth Factor Isolation Medium Tube Formation Assay Human Microvascular Endothelial Cell Modify Boyden Chamber
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