Methods to Study Angiogenesis in vitro

  • Katrin Lamszus
  • Oliver Heese
  • Manfred Westphal
Part of the Cancer Treatment and Research book series (CTAR, volume 117)

Abstract

Human microvascular endothelial cells (ECs) are more difficult to isolate than macrovascular ECs. Pure cultures of human macrovascular ECs can be obtained easily from umbilical cord veins by incubation and subseqent flushing of the veins with enzymatic solutions (4). Cultures of human umbilical vein ECs (HUVECs) can be greatly expanded and usually retain markers of endothelial differentiation over more than ten passages until they eventually become senescent.

Keywords

Permeability Migration Heparin Penicillin Dimethyl 

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Copyright information

© Springer Science+Business Media New York 2004

Authors and Affiliations

  • Katrin Lamszus
    • 1
  • Oliver Heese
    • 1
  • Manfred Westphal
    • 1
  1. 1.Department of NeurosurgeryUniversity Hospital Hamburg-EppendorfGermany

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