Methods to Study Angiogenesis in vitro

  • Katrin Lamszus
  • Oliver Heese
  • Manfred Westphal
Part of the Cancer Treatment and Research book series (CTAR, volume 117)


Human microvascular endothelial cells (ECs) are more difficult to isolate than macrovascular ECs. Pure cultures of human macrovascular ECs can be obtained easily from umbilical cord veins by incubation and subseqent flushing of the veins with enzymatic solutions (4). Cultures of human umbilical vein ECs (HUVECs) can be greatly expanded and usually retain markers of endothelial differentiation over more than ten passages until they eventually become senescent.


Vascular Endothelial Growth Factor Isolation Medium Tube Formation Assay Human Microvascular Endothelial Cell Modify Boyden Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bowman PD, Betz AL, Ar D, Wolinsky JS, Penney JB, Shivers RR, Goldstein GW: Primary culture of capillary endothelium from rat brain. In Vitro 1981, 17:353-62.PubMedCrossRefGoogle Scholar
  2. 2.
    Folkman J, Klagsbrun M: Angiogenic factors. Science 1987, 235:442-7.PubMedCrossRefGoogle Scholar
  3. 3.
    Furie MB, Cramer EB, Naprstek BL, Silverstein SC: Cultured endothelial cell monolayers that restrict the transendothelial passage of macromolecules and electrical current. J Cell Biol 1984, 98:1033-41.PubMedCrossRefGoogle Scholar
  4. 4.
    Jaffe EA, Nachman RL, Becker CG, Minick CR: Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973, 52:2745-56.PubMedCrossRefGoogle Scholar
  5. 5.
    Lamszus K, Schmidt NO, Ergun S, Westphal M: Isolation and culture of human neuromicrovascular endothelial cells for the study of angiogenesis in vitro. J Neurosci Res 1999, 55:370-81.PubMedCrossRefGoogle Scholar
  6. 6.
    Lamszus K, Schmidt NO, Jin L, Laterra J, Zagzag D, Way D, Witte M, Weinand M, Goldberg ID, Westphal M, Rosen EM: Scatter factor promotes motility of human glioma and neuromicrovascular endothelial cells. Int J Cancer 1998, 75:19-28.PubMedCrossRefGoogle Scholar
  7. 7.
    Montesano R, Orci L: Tumor-promoting phorbol esters induce angiogenesis in vitro. Cell 1985, 42:469-77.PubMedCrossRefGoogle Scholar
  8. 8.
    Schmidt NO, Westphal M, Hagel C, Ergun S, Stavrou D, Rosen EM, Lamszus K: Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis. Int J Cancer 1999, 84:10-8.PubMedCrossRefGoogle Scholar
  9. 9.
    Zetter BR: Assay of capillary endothelial cell migration. Methods Enzymol 1987, 147:135-44.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2004

Authors and Affiliations

  • Katrin Lamszus
    • 1
  • Oliver Heese
    • 1
  • Manfred Westphal
    • 1
  1. 1.Department of NeurosurgeryUniversity Hospital Hamburg-EppendorfGermany

Personalised recommendations