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Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine pp 361–384Cite as

Human Mycoses: The Role of Molecular Biology

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Abstract

Since the early 1980s a dramatic rise in the incidence of invasive disease due to the filamentous fungi (molds) has occurred (Latgé, 1999). Because these fungi are primarily opportunistic pathogens, in large part this trend can be attributed to the increase in the number of patients undergoing highly immunosuppressive therapies including chemotherapy and solid organ and bone marrow transplantation. While the list of organisms infecting immunocompromised hosts is long, the vast majority of these infections are due to Aspergillus species. Among these species, Aspergillus fumigatus accounts for almost 90% of cases (Latgé, 1999). For those infections not due to Aspergillus sp., zygomycetes, Pseudallescheria boydii, and Fusarium sp., are also common pathogens. In this chapter, we will review advances in the study of these organisms as pathogens, particularly the progress made possible by the application of the techniques of molecular biology. We will focus our attention primarily on Aspergillus sp. and the zygomycetes as the most important pathogens in this group. While there has been substantial research on Fusarium sp., it has been predominately on the role of this organism in plant pathogenicity and is beyond the scope of this chapter.

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References

  • Al-Asiri, R.H., van Dijken, P.J., Mahmood, M.A., Al-Shahed, M.S., Rossi, M.L., and Osoba, A.O. (1996). Isolated hepatic mucormycosis in an immunocompetent child. Am. J. Gastroenterol. 91, 606–607.

    PubMed  CAS  Google Scholar 

  • Amutan, M., Nyyssonen, E., Stubbs, J., Diaz-Torres, M.R., and Dunn-Coleman, N. (1996). Identification and cloning of a mobile transposon from Aspergillus niger var. awamori. Curr. Genet. 29, 468–473.

    PubMed  CAS  Google Scholar 

  • Ashikari, T., Amachi, T., Yoshizumi, H., Horiuchi, H., Takagi, M., and Yano, K. (1990). Correct splicing of modified introns of a Rhizopus proteinase gene in Saccharomyces cerevisiae. Mol. Gen. Genet. 223, 11–16.

    PubMed  CAS  Google Scholar 

  • Aufauvre-Brown, A., Brown, J.S., and Holden, D.W. (1998). Comparison of virulence between clinical and environmental isolates of Aspergillus fumigatus. Eur. J. Clin. Microbiol. Infect. Dis. 17, 778–780.

    PubMed  CAS  Google Scholar 

  • Aufauvre-Brown, A., Mellado, E., Gow, N.A.R., and Holden, D.W. (1997). Aspergillus fumigatus chs E: A gene related to CHS3 of Saccharomyces cerevisiae and important for hyphal growth and conidiophore development but not pathogenicity. Fungal Genet. Biol. 21, 141–152.

    PubMed  CAS  Google Scholar 

  • Blakeslee, A.F. (1906). Zygospore germination in the Mucorineae. Ann. Mycol. 4, 1–28.

    Google Scholar 

  • Bodey, G., Bueltmann, B., Duguid, W., Gibbs, D., Hanak, H., Hotchi, M., Mall, G., Martino, P. et al. (1992). Fungal infections in cancer patients: An international autopsy survey. Eur. J. Clin. Microbiol. Infect. Dis. 11, 99–109.

    PubMed  CAS  Google Scholar 

  • Brakhage, A.A. and Langfelder, K. (2002). Menacing mold: The molecular biology of Aspergillus fumigatus. Annu. Rev. Microbiol. 56, 433–455.

    PubMed  CAS  Google Scholar 

  • Brookman, J.L. and Denning, D.W. (2000). Molecular genetics in Aspergillus fumigatus. Curr. Opin. Microbiol. 3, 468–474.

    PubMed  CAS  Google Scholar 

  • Brown, J.S., Aufauvre-Brown, A., Brown, J., Jennings, J.M., Arst, H., Jr., and Holden, D.W. (2000). Signaturetagged and directed mutagenesis identify PABA synthetase as essential for Aspergillus fumigatus pathogenicity. Mol. Microbiol. 36, 1371–1380.

    PubMed  CAS  Google Scholar 

  • Brown, J.S., Aufauvre-Brown, A., and Holden, D.W. (1998). Insertional mutagenesis of Aspergillus fumigatus. Mol. Gen. Genet. 259, 327–335.

    PubMed  CAS  Google Scholar 

  • Çagatay, A.A., Öncü, S.S., Çalangu, S.S., Yildirmak, T.T., Özsüt, H.H., and Eraksoy, H.H. (2001). Rhinocerebral mucormycosis treated with 32 gram liposomal amphotericin B and incomplete surgery: A case report. BMC Infect. Dis. 1, 22.

    PubMed  Google Scholar 

  • Chakraborty, B.N., Patterson, N.A., and Kapoor, M. (1991). An electroporation-based system for high-efficiency transformation of germinated conidia of filamentous fungi. Can. J. Microbiol. 37, 858–863.

    PubMed  CAS  Google Scholar 

  • Clemons, K.V., Miller, T.K., Selitrennikoff, C.P., and Stevens, D.A. (2002). fos-1, a putative histidine kinase as a virulence factor for systemic aspergillosis. Med. Mycol. 40, 259–262.

    PubMed  CAS  Google Scholar 

  • Cormack, B.P., Bertram, G., Egerton, M., Gow, N.A., Falkow, S., and Brown, A.J. (1997). Yeast-enhanced green fluorescent protein (yEGFP) a reporter of gene expression in Candida albicans. Microbiology 143, 303–311.

    PubMed  CAS  Google Scholar 

  • de Groot, M.J., Bundock, P., Hooykaas, P.J., and Beijersbergen, A.G. (1998). Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Naure Biotechnol. 16, 839–842.

    Google Scholar 

  • de Lucas, J.R., Dominguez, A.I., Higuero, Y., Martinez, O., Romero, B., Mendoza, A., Garcia-Bustos, J.F., and Laborda, F. (2001). Development of a homologous transformation system for the opportunistic human pathogen Aspergillus fumigatus based on the sC gene encoding ATP sulfurylase. Arch. Microbiol. 176, 106–113.

    PubMed  Google Scholar 

  • del Poeta, M., Toffaletti, D.L., Rude, T.H., Sparks, S.D., Heitman, J., and Perfect, J.R. (1999). Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 67, 1812–1820.

    PubMed  Google Scholar 

  • del Valle-Zapico, A., Rubio-Suarez, A., Mellado-Encinas, P., Morales-Angulo, C., and Cabrera-Pozuelo, E. (1996). Mucormycosis of the sphenoid sinus in an otherwise healthy patient. Case report and literature review. J. Laryngol. Otol. 110, 471–473.

    PubMed  Google Scholar 

  • d’Enfert, C. (1996). Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5′-decarboxylase gene, pyrG, as a unique transformation marker. Curr. Genet. 30, 76–82.

    PubMed  Google Scholar 

  • d’Enfert, C., Diaquin, M., Delit, A., Wuscher, N., Debeaupuis, J.R, Huerre, M., and Latgé, J.P. (1996). Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus. Infect. Immun. 64, 4401–4405.

    PubMed  Google Scholar 

  • Denning, D.W. (1996). Therapeutic outcome in invasive aspergillosis. Clin. Infect. Dis. 23, 608–615.

    PubMed  CAS  Google Scholar 

  • Denning, D.W. (2000). Aspergillus species. In G.L. Mandell, J.E. Bennett and R. Dolin (eds.) Mandell, Douglas, and Bennett’s Principals and Practice of Infectious Diseases, 5th edn. Churchill Livingston, Philadelphia, pp. 2674–2685.

    Google Scholar 

  • Denning, D.W., Anderson, M.J., Turner, G., Latgé, J.P., and Bennett, J.W. (2002). Sequencing the Aspergillus fumigatus genome. Lancet Infect. Dis. 2, 251–253.

    PubMed  CAS  Google Scholar 

  • Ericsson, M., Anniko, M., Gustafsson, H., Hjalt, C.A., Stenling, R., and Tarnvik, A. (1993). A case of chronic progressive rhinocerebral mucormycosis treated with liposomal amphotericin B and surgery. Clin Infect Dis. 16, 585–586.

    PubMed  CAS  Google Scholar 

  • Firon, A., Beauvais, A., Latgé, J.P., Couve, E., Grosjean-Cournoyer, M.C., and d’Enfert, C. (2002). Characterization of essential genes by parasexual genetics in the human fungal pathogen Aspergillus fumigatus. Impact of genomic rearrangements associated with electroporation of DNA. Genetics 161, 1077–1087.

    PubMed  CAS  Google Scholar 

  • Fu, Y., Rieg, G., Fonzi, WA., Belanger, PH., Edwards, J.E., Jr., and Filler, S.G. (1998). Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells. Infect. Immun. 66, 1783–1786.

    PubMed  CAS  Google Scholar 

  • Fungaro, M.H., Rech, E., Muhlen, G.S., Vainstein, M.H., Pascon, R.C., de Queiroz, M.V., Pizzirani-Kleiner, A.A., and de Azevedo, J.L. (1995). Transformation of Aspergillus nidulans by microprojectile bombardment on intact conidia. FEMS Microbiol. Lett. 125, 293–297.

    PubMed  CAS  Google Scholar 

  • Gatignol, A., Baron, M., and Tiraby, G. (1987). Phleomycin resistance encoded by the ble gene from transposon Tn 5 as a dominant selectable marker in Saccharomyces cerevisiae. Mol. Gen. Genet. 207, 342–348.

    PubMed  CAS  Google Scholar 

  • Gaur, N.K., Klotz, S.A., and Henderson, R.L. (1999). Overexpression of the Candida albicans ALA1 gene in Saccharomyces cerevisiae results in aggregation following attachment of yeast cells to extracellular matrix proteins, adherence properties similar to those of Candida albicans. Infect. Immun. 67, 6040–6047.

    PubMed  CAS  Google Scholar 

  • Giron, J., Sans, N., Poey, C., Fajadet, P., Fourcade, D., Senac, J.P., and Railhac, J.J. (1998). CT-guided percutaneous treatment of inoperable pulmonary aspergilloma. Apropos of 42 cases. J. Radiol. 79, 139–145.

    PubMed  CAS  Google Scholar 

  • Glayzer, D.C., Roberts, I.N., Archer, D.B., and Oliver, R.P. (1995). The isolation of Ant1, a transposable element from Aspergillus niger. Mol. Gen. Genet. 249, 432–438.

    PubMed  CAS  Google Scholar 

  • Gonzalez, A., Jimenez, A., Vazquez, D., Davies, J.E., and Schindler, D. (1978). Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim. Biophys. Acta 521, 459–469.

    PubMed  CAS  Google Scholar 

  • Hector, R.F., Yee, E., and Collins, M.S. (1990). Use of DBA/2N mice in models of systemic candidiasis and pulmonary and systemic aspergillosis. Infect. Immun. 58, 1476–1478.

    PubMed  CAS  Google Scholar 

  • Hensel, ML, Arst, H.N., Jr., Aufauvre-Brown, A., and Holden, D.W. (1998). The role of the Aspergillus fumigatus areA gene in invasive pulmonary aspergillosis. Mol. Gen. Genet. 258, 553–557.

    PubMed  CAS  Google Scholar 

  • Herbrecht, R., Denning, D.W., Patterson, T.F., Bennett, J.E., Greene, R.E., Oestmann, J.-W, Kern, W.V., Marr, K.A. et al. (2002). Voriconazole versus amphotericin B for primary therapy of invasive aspergillosis. New Engl. J. Med. 347, 408–415.

    PubMed  CAS  Google Scholar 

  • Horiuchi, H., Ashikari, T., Amachi, T., Yoshizumi, H., Takagi, M., and Yano, K. (1990). High-level secretion of a Rhizopus niveus aspartic proteinase in Saccharomyces cerevisiae. Agric. Biol. Chem. 54, 1771–1779.

    PubMed  CAS  Google Scholar 

  • Horiuchi, H., Takaya, N., Yanai, K., Nakamura, M., Ohta, A., and Takagi, M. (1995). Cloning of the Rhizopus niveus pyr4 gene and its use for the transformation of Rhizopus delemar. Curr. Genet. 27, 472–478.

    PubMed  CAS  Google Scholar 

  • Hospenthal, D.R., Kwon-Chung, K.J., and Bennett, J.E. (1998). Concentrations of airborne Aspergillus compared to the incidence of invasive aspergillosis: Lack of correlation. Med. Mycol. 36, 165–168.

    PubMed  CAS  Google Scholar 

  • Ibrahim-Granet, O. and d’Enfert, C. (1997). The Aspergillus fumigatus mepB gene encodes an 82 kDa intracellular metalloproteinase structurally related to mammalian thimet oligopeptidases. Microbiology 143, 2247–2253.

    PubMed  CAS  Google Scholar 

  • Ikegami, Y., Amitani, R., Murayama, T., Nawada, R., Lee, W.J., Kawanami, R., and Kuze, F. (1998). Effects of alkaline protease or restrictocin deficient mutants of Aspergillus fumigatus on human polymorphonuclear leukocytes. Eur. Respir. J. 12, 607–611.

    PubMed  CAS  Google Scholar 

  • Kohno, M., Enatsu, M., and Kugimiya, W. (1998). Cloning of genomic DNA of Rhizopus niveus lipase and expression in the yeast Saccharomyces cerevisiae. Biosci. Biotechnol. Biochem. 62, 2425–2427.

    PubMed  CAS  Google Scholar 

  • Kohno, M., Enatsu, M., Yoshiizumi, M., and Kugimiya, W. (1999). High-level expression of Rhizopus niveus lipase in the yeast Saccharomyces cerevisiae and structural properties of the expressed enzyme. Protein Expr. Purif. 15, 327–335.

    PubMed  CAS  Google Scholar 

  • Kontoyiannis, D.P. (1999). Genetic analysis of azole resistance by transposon mutagenesis in Saccharomyces cerevisiae. Antimicrob. Agents Chemother. 43, 2731–2735.

    PubMed  CAS  Google Scholar 

  • Kothary, M.H., Chase, T., Jr., and Macmillan, J.D. (1984). Correlation of elastase production by some strains of Aspergillus fumigatus with ability to cause pulmonary invasive aspergillosis in mice. Infect. Immun. 43, 320–325.

    PubMed  CAS  Google Scholar 

  • Langfelder, K., Gattung, S., and Brakhage, A.A. (2002). A novel method used to delete a new Aspergillus fumigatus ABC transporter-encoding gene. Curr. Genet. 41, 268–274.

    PubMed  CAS  Google Scholar 

  • Langfelder, K., Philippe, B., Jahn, B., Latgé, J.P., and Brakhage, A.A. (2001). Differential expression of the Aspergillus fumigatus pksP gene detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 69, 6411–6418.

    PubMed  CAS  Google Scholar 

  • Latgé, J.P. (1999). Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 12, 310–350.

    PubMed  Google Scholar 

  • Lebeau, B., Pelloux, H., Pinel, C., Michallet, M., Gout, J.P., Pison, C., Delormas, P., Bru, J.P, et al. (1994). Itraconazole in the treatment of aspergillosis: A study of 16 cases. Mycoses 37, 171–179.

    PubMed  CAS  Google Scholar 

  • Levadoux, W.L., Gregory, K.F., and Taylor, A. (1981). Sequential cold-sensitive mutations in Aspergillus fumigatus. II. Analysis by the parasexual cycle. Can. J. Microbiol. 27, 295–303.

    PubMed  CAS  Google Scholar 

  • Li Destri Nicosia, M.G., Brocard-Masson, C., Demais, S., Hua-Van, A., Daboussi, M.J., and Scazzocchio, C. (2001). Heterologous transposition in Aspergillus nidulans. Mol. Microbiol. 39, 1330–1344.

    PubMed  CAS  Google Scholar 

  • Liou, C.M., Yanai, K., Horiuchi, H., and Takagi, M. (1992). Transformation of a Leu− mutant of Rhizopus niveus with the leuA gene of Mucor circinelloides. Biosci. Biotechnol. Biochem. 56, 1503–15

    PubMed  CAS  Google Scholar 

  • Lueg, E.A., Ballagh, R.H., and Forte, V. (1996). Analysis of the recent cluster of invasive fungal sinusitis at the Toronto Hospital for Sick Children. J. Otolaryngol. 25, 366–370.

    PubMed  CAS  Google Scholar 

  • McDade, H.C. and Cox, G.M. (2001). A new dominant selectable marker for use in Cryptococcus neoformans. Med. Mycol. 39, 151–154.

    PubMed  CAS  Google Scholar 

  • Mellado, E., Aufauvre-Brown, A., Gow, N.A., and Holden, D.W. (1996a). The Aspergillus fumigatus chsC and chsG genes encode class III chitin synthases with different functions. Mol. Microbiol. 20, 667–679.

    PubMed  CAS  Google Scholar 

  • Mellado, E., Specht, C.A., Robbins, P.W., and Holden, D.W. (1996b). Cloning and characterization of chsD, a chitin synthase-like gene of Aspergillus fumigatus. FEMS Microbiol. Lett. 143, 69–76.

    PubMed  CAS  Google Scholar 

  • Michel, S., Ushinsky, S., Klebl, B., Leberer, E., Thomas, D., Whiteway, M., and Morschhäuser, J. (2002). Generation of conditional lethal Candida albicans mutants by inducible deletion of essential genes. Mol. Microbiol. 46, 269–280.

    PubMed  CAS  Google Scholar 

  • Mullins, E.D. and Kang, S. (2001). Transformation: A tool for studying fungal pathogens of plants. Cell. Mol. Life Sci. 58, 2043–2052.

    PubMed  CAS  Google Scholar 

  • Mullins, J., Harvey, R., and Seaton, A. (1976). Sources and incidence of airborne Aspergillus fumigatus (Fres). Clin. Allergy 6, 209–217.

    PubMed  CAS  Google Scholar 

  • Mullins, J. and Seaton, A. (1978). Fungal spores in lung and sputum. Clin. Allergy 8, 525–533.

    PubMed  CAS  Google Scholar 

  • Neuveglise, C., Sarfati, J., Latgé, J.P., and Paris, S. (1996). Afut1, a retrotransposon-like element from Aspergillus fumigatus. Nucleic Acids Res. 24, 1428–14

    PubMed  CAS  Google Scholar 

  • Notz, R., Maurhofer, M., Dubach, H., Haas, D., and Defago, G. (2002). Fusaric acid-producing strains of Fusarium oxysporum alter 2,4-diacetylphloroglucinol biosynthetic gene expression in Pseudomonas fluorescens CHAO in vitro and in the rhizosphere of wheat. Appl. Environ. Microbiol. 68, 2229–2235.

    PubMed  CAS  Google Scholar 

  • Nyyssonen, E., Amutan, M., Enfield, L., Stubbs, J., and Dunn-Coleman, N.S. (1996). The transposable element Tan1 of Aspergillus niger var. awamori, a new member of the Fot1 family. Mol. Gen. Genet. 253, 50–56.

    PubMed  CAS  Google Scholar 

  • O’Connell, M.A., Pluss, J.L., Schkade, P., Henry, A.R., and Goodman, D.L. (1995). Rhizopus-induced hypersensitivity pneumonitis in a tractor driver. J. Allergy Clin. Immunol. 95, 779–780.

    PubMed  Google Scholar 

  • Ozeki, K., Kyoya, F., Hizume, K., Kanda, A., Hamachi, M., and Nunokawa, Y. (1994). Transformation of intact Aspergillus niger by electroporation. Biosci. Biotechnol. Biochem. 58, 2224–2227.

    PubMed  CAS  Google Scholar 

  • Paris, S. and Latgé, J.P. (2001). Afutl, a new family of degenerate gypsy-like retrotransposon from Aspergillus fumigatus. Med. Mycol. 39, 195–198.

    PubMed  CAS  Google Scholar 

  • Parta, M., Chang, Y., Rùlong, S., Pinto-DaSilva, P., and Kwon-Chung, K.J. (1994). HYP1, a hydrophobin gene from Aspergillus fumigatus, complements the rodletless phenotype in Aspergillus nidulans. Infect. Immun. 62, 4389–4395.

    PubMed  CAS  Google Scholar 

  • Punt, P.J., Oliver, R.P., Dingemanse, M.A., Pouwels, PH., and van den Hondel, C.A. (1987). Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56, 117–124.

    PubMed  CAS  Google Scholar 

  • Punt, P.J. and van den Hondel, C.A. (1992). Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. Methods Enzymol. 216, 447–457.

    PubMed  CAS  Google Scholar 

  • Ribes, J.A., Vanover-Sams, C.L., and Baker, D.J. (2000). Zygomycetes in human disease. Clin. Microbiol. Rev. 13,236–301.

    PubMed  CAS  Google Scholar 

  • Richardson, M.D. and Shankland, G.S. (1999). Rhisopus, Rhizomucor, Absidia, and other agents of systemic and subcutaneous zygomycoses. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (eds) Manual of Clinical Microbiology, 5th edn. ASM Press, Washington DC., pp. 1242–1258.

    Google Scholar 

  • Roncero, M.I. (1984). Enrichment method for the isolation of auxotrophic mutants of Mucor using the polyene antibiotic N-glycosylpolyfungin. Carlsberg Res. Commun. 49, 658–690.

    Google Scholar 

  • Roncero, M.I., Jepsen, L.P., Stroman, P., and van Heeswijck, R. (1989). Characterization of a leuA gene and an ARS element from Mucor circinelloides. Gene 84, 335–343.

    PubMed  CAS  Google Scholar 

  • Ruiz-Diez, B. and Martinez-Suarez, J.V. (1999). Electrotransformation of the human pathogenic fungus Scedosporium prolificans mediated by repetitive rDNA sequences. FEMS Immunol. Med. Microbiol. 25, 275–282.

    PubMed  CAS  Google Scholar 

  • Rupp, S. (2002). lacZ assays in yeast. Methods Enzymol. 350, 112–131.

    PubMed  CAS  Google Scholar 

  • Sanchez, O., Navarro, R.E., and Aguirre, J. (1998). Increased transformation frequency and tagging of developmental genes in Aspergillus nidulans by restriction enzyme-mediated integration (REMI). Mol. Gen. Genet. 258, 89–94.

    PubMed  CAS  Google Scholar 

  • Sandhu, D.K., Sandhu, R.S., Khan, Z.U., and Damodaran, V.N. (1976). Conditional virulence of a p-aminobenzoic acid-requiring mutant of Aspergillus fumigatus. Infect. Immun. 13, 527–532.

    PubMed  CAS  Google Scholar 

  • Schaffner, A. (1994). Macrophage-Aspergillis interactions. Immunol. Ser. 60, 545–552.

    PubMed  CAS  Google Scholar 

  • Schaffner, A., Douglas, H., and Braude, A. (1982). Selective protection against conidia by mononuclear and against mycelia by polymorphonuclear phagocytes in resistance to Aspergillus. Observations on these two lines of defense in vivo and in vitro with human and mouse phagocytes. J. Clin. Invest. 69, 617–631.

    PubMed  CAS  Google Scholar 

  • Skory, C.D. (2002). Homologous recombination and double-strand break repair in the transformation of Rhizopus oryzae. Mol. Genet. Genomics 268, 397–406.

    PubMed  CAS  Google Scholar 

  • Smith, J.M., Tang, C.M., van Noorden, S., and Holden, D.W. (1994). Virulence of Aspergillus fumigatus double mutants lacking restriction and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis. Infect. Immun. 62, 5247–5254.

    PubMed  CAS  Google Scholar 

  • Spellig, T., Bottin, A., and Kahmann, R. (1996). Green fluorescent protein (GFP) as a new vital marker in the phytopathogenic fungus Ustilago maydis. Mol. Gen. Genet. 252, 503–509.

    PubMed  CAS  Google Scholar 

  • Stevens, D.A., Kan, V.L., Judson, M.A., Morrison, VA., Dummer, S., Denning, D.W., Bennett, J.E., Walsh, T.J. et al. (2000). Practice guidelines for diseases caused by Aspergillus. Infectious Diseases Society of America. Clin. Infect. Dis. 30, 696–709.

    PubMed  CAS  Google Scholar 

  • Suarez, T., Orejas, M., and Eslava, A.P. (1985). Isolation, regeneration, and fusion of Phycomyces blakesleeanus spheroplasts. Exp. Mycol. 9, 203–211.

    Google Scholar 

  • Sugar, A.M. (2000). Agents of mucormycosis and related species. In G.L. Mandell, J.E. Bennett, and R. Dolin (eds) Mandell, Douglas, and Bennett’s Principals and Practice of Infectious Diseases, 5th edn. Churchill Livingston, Philadelphia, pp. 2685–2695.

    Google Scholar 

  • Sundstrom, P., Cutler, J.E., and Staab, J.F. (2002). Reevaluation of the role of HWP1 in systemic candidiasis by use of Candida albicans strains with selectable marker URA3 targeted to the ENO1 locus. Infect. Immun. 70, 3281–3283.

    PubMed  CAS  Google Scholar 

  • Takaya, N., Yanai, K., Horiuchi, H., Ohta, A., and Takagi, M. (1994). Cloning and characterization of two 3-phosphoglycerate kinase genes of Rhizopus niveus and heterologous gene expression using their promoters. Curr. Genet. 25, 524–530.

    PubMed  CAS  Google Scholar 

  • Takaya, N., Yanai, K., Horiuchi, H., Ohta, A., and Takagi, M. (1995). Analysis of the 3-phosphoglycerate kinase 2 promoter in Rhizopus niveus. Gene 152, 121–125.

    PubMed  CAS  Google Scholar 

  • Takaya, N., Yanai, K., Horiuchi, H., Ohta, A., and Takagi, M. (1996). Cloning and characterization of the Rhizopus niveus leu1 gene and its use for homologous transformation. Biosci. Biotechnol. Biochem. 60, 448–452.

    PubMed  CAS  Google Scholar 

  • Thau, N., Monod, M., Crestani, B., Rolland, C., Tronchin, G., Latgé, J.P., and Paris, S. (1994). Rodletless mutants of Aspergillus fumigatus. Infect. Immun. 62, 4380–4388.

    PubMed  CAS  Google Scholar 

  • Tornee, J.F. and Kauffman, H.F. (2000). Putative virulence factors of Aspergillus fumigatus. Clin. Exp. Allergy 30, 476–484.

    Google Scholar 

  • Tsai, H.F, Chang, Y.C., Washburn, R.G., Wheeler, M.H., and Kwon-Chung, K.J. (1998). The developmentally regulated alb1 gene of Aspergillus fumigatus: Its role in modulation of conidial morphology and virulence. J. Bacteriol. 180, 3031–3038.

    PubMed  CAS  Google Scholar 

  • Tsai, H.F., Wheeler, M.H., Chang, Y.C., and Kwon-Chung, K.J. (1999). A developmentally regulated gene cluster involved in conidial pigment biosynthesis in Aspergillus fumigatus. J. Bacteriol. 181, 6469–6477.

    PubMed  CAS  Google Scholar 

  • van Heeswijck, R. (1984). The formation of protoplasts from Mucor species. Carlberg Res. Commun. 49, 597–609.

    Google Scholar 

  • van Heeswijck, R., Roncero, M.I., and Jepsen, L.P. (1998). Genetic analysis and manipulation of Mucor species by DNA-mediated transformation. In H.F. Linskens and J.F. Jackson (eds.) Modern Methods of Plant Analysis (New Series Vol. 7). Springer Verlag, pp. 207–220.

    Google Scholar 

  • Villalba, F., Lebrun, M.H., Hua-Van, A., Daboussi, M.J., and Grosjean-Cournoyer, M.C. (2001). Transposon impala, a novel tool for gene tagging in the rice blast fungus Magnaporthe grisea. Mol. Plant Microbe Interact. 14, 308–315.

    PubMed  CAS  Google Scholar 

  • Wasylnka, J.A. and Moore, M.M. (2002). Uptake of Aspergillus fumigatus conidia by phagocytic and nonphagocytic cells in vitro: Quantitation using strains expressing green fluorescent protein. Infect. Immun. 70, 3156–3163.

    PubMed  CAS  Google Scholar 

  • Watanabe, A., Fujii, I., Tsai, H., Chang, Y.C., Kwon-Chung, K.J., and Ebizuka, Y. (2000). Aspergillus fumigatus alb1 encodes naphthopyrone synthase when expressed in Aspergillus oryzae. FEMS Microbiol. Lett. 192, 39–44.

    PubMed  CAS  Google Scholar 

  • Weidner, G., d’Enfert, C., Koch, A., Mol, P.C., and Brakhage, A.A. (1998). Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5′ monophosphate decarboxylase. Curr. Genet. 33, 378–385.

    PubMed  CAS  Google Scholar 

  • Weidner, G., Steidl, S., and Brakhage, A.A. (2001). The Aspergillus nidulans homoaconitase gene lysF is negatively regulated by the multimeric CCAAT-binding complex AnCF and positively regulated by GATA sites. Arch. Microbiol. 175, 122–132.

    PubMed  CAS  Google Scholar 

  • Weng, D.E., Wilson, W.H., Little, R., and Walsh, T.J. (1998). Successful medical management of isolated renal zygomycosis: Case report and review. Clin. Infect. Dis. 26, 601–605.

    PubMed  CAS  Google Scholar 

  • Willins, D.A., Kessler, M., Walker, S.S., Reyes, G.R., and Cottarel, G. (2002a). Genomics strategies for antifungal drug discovery—from gene discovery to compound screening. Curr. Pharm. Des. 8, 1137–1154.

    PubMed  CAS  Google Scholar 

  • Willins, D.A., Shimer, G.H., Jr., and Cottarel, G. (2002b). A system for deletion and complementation of Candida glabrata genes amenable to high-throughput application. Gene 292, 141–149.

    PubMed  CAS  Google Scholar 

  • Wimander, K. and Belin, L. (1980). Recognition of allergic alveolitis in the trimming department of a Swedish saw mill. Eur. J. Respir. Dis. Suppl (107), 163–167.

    Google Scholar 

  • Wostemeyer, J., Burmester, A., and Weigel, C. (1987). Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca. Curr. Genet. 12, 625–627.

    PubMed  CAS  Google Scholar 

  • Wysong, D.R., Christin, L., Sugar, A.M., Robbins, P.W., and Diamond, R.D. (1998). Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene. Infect. Immun. 66, 1953–1961.

    PubMed  CAS  Google Scholar 

  • Yamazaki, H., Ohnishi, Y., Takeuchi, K., Mori, N., Shiraishi, N., Sakata, Y., Suzuki, H., and Horinouchi, S. (1999). Genetic transformation of a Rhizomucor pusillus mutant defective in asparagine-linked glycosylation: Production of a milk-clotting enzyme in a less-glycosylated form. Appl. Microbiol. Biotechnol. 52, 401–409.

    PubMed  CAS  Google Scholar 

  • Yanai, K., Horiuchi, H., Takagi, M., and Yano, K. (1991). Transformation of Rhizopus niveus using a bacterial blasticidin S resistance gene as a dominant selectable marker. Curr. Genet. 19, 221–226.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Infectious Diseases, Harbor-UCLA Research and Education Institute, 1000 W. Carson St., Torrance, CA, 90502

    Donald C. Sheppard, Ashraf S. Ibrahim & John E. Edwards . Jr

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  1. Donald C. Sheppard
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  2. Ashraf S. Ibrahim
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  3. John E. Edwards . Jr
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Editors and Affiliations

  1. Department of Biologics Research, Merck Research Laboratories, Rahway, New Jersey

    Jan S. Tkacz

  2. Department of Molecular Biotechnology Novozymes A/S, Bagsvaerd, Denmark

    Lene Lange

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Sheppard, D.C., Ibrahim, A.S., Edwards ., J.E. (2004). Human Mycoses: The Role of Molecular Biology. In: Tkacz, J.S., Lange, L. (eds) Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8859-1_14

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