Abstract
Information about the morphomechanical changes during cell swelling could be a basis for understanding sensoring in volume regulatory signal transduction. We therefore observed living human keratinocytes of the line Hacat (Boukamp et al. 1988) during RVD with reflection scanning acoustic microscopy using an ELS AM operated at 1 Ghz. The aquired images clearly showed topographic changes during cell swelling and RVD. A transient, reversible decrease in ultrasound velocity profiles could be determined from extrema of interference fringes using an iterative algorithm method (Litniewski and Bereiter-Hahn, 1990). This indicates a reversible change in physical properties of the cytocortex which could be the mechanical basis for the unknown volume sensor of animal cells.
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© 1996 Springer Science+Business Media New York
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Yastas, S., Bereiter-Hahn, J. (1996). Acoustical Imaging of Living Cells During Volume Regulation. In: Tortoli, P., Masotti, L. (eds) Acoustical Imaging. Acoustical Imaging, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8772-3_35
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DOI: https://doi.org/10.1007/978-1-4419-8772-3_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4687-6
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