Acoustical Imaging of Living Cells During Volume Regulation

  • Samir Yastas
  • Jürgen Bereiter-Hahn
Part of the Acoustical Imaging book series (ACIM, volume 22)

Abstract

Information about the morphomechanical changes during cell swelling could be a basis for understanding sensoring in volume regulatory signal transduction. We therefore observed living human keratinocytes of the line Hacat (Boukamp et al. 1988) during RVD with reflection scanning acoustic microscopy using an ELS AM operated at 1 Ghz. The aquired images clearly showed topographic changes during cell swelling and RVD. A transient, reversible decrease in ultrasound velocity profiles could be determined from extrema of interference fringes using an iterative algorithm method (Litniewski and Bereiter-Hahn, 1990). This indicates a reversible change in physical properties of the cytocortex which could be the mechanical basis for the unknown volume sensor of animal cells.

Keywords

Penicillin Streptomycin Cytosol Hepes Gelatin 

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Samir Yastas
    • 1
  • Jürgen Bereiter-Hahn
    • 1
  1. 1.Cinematic Cell Research, BiozentrumFrankfurt am Main

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