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Molecular heterogeneity and regulation of poly(ADP-ribose) glycohydrolase

  • Jean-Christophe Amé
  • Elaine L. Jacobson
  • Myron K. Jacobson
Part of the Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease book series (DMCB, volume 30)

Abstract

We have recently described the isolation and characterization of bovine cDNA encoding poly(ADP-ribose) glycohydrolase (PARG). We describe here the preparation and characterization of antibodies to PARG. These antibodies have been used to demonstrate the presence of multiple forms of PARG in tissue and cell extracts from bovine, rat, mouse, and insects. Our results indicate that multiple forms of PARG previously reported could result from a single gene. Analysis of PARG in cells in which poly(ADP-ribose) polymerase (PARP) has been genetically inactivated indicates that the cellular content of PARG is regulated independently of PARP.

Key words

poly(ADP-ribose) glycohydrolase antibody expression ADP-ribose polymer metabolism DNA repair 

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Jean-Christophe Amé
    • 1
  • Elaine L. Jacobson
    • 2
    • 3
    • 4
    • 5
  • Myron K. Jacobson
    • 1
    • 3
  1. 1.Division of Pharmaceutical SciencesCollege of PharmacyLexingtonUSA
  2. 2.Department of Clinical SciencesLexingtonUSA
  3. 3.Lucille P. Markey Cancer CenterLexingtonUSA
  4. 4.Multidisciplinary Ph.D. Program in Nutritional Sciences, University of KentuckyLexingtonUSA
  5. 5.College of Pharmacy, University of KentuckyLexingtonUSA

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