Proteolytic Degradation of Poly(ADP-ribose)polymerase by Contaminating Proteases in Commercial Preparations of DNAse I

  • Rafael Alvarez-Gonzalez
  • Guy G. Poirier
  • Marcos Martinez
Conference paper

Abstract

The stimulation of poly(ADP-ribose)polymerase (PADPRP) activity by the addition of agents that induce DNA damage and formation of DNA strand-breaks in vitro and in vivo is well established (1). Not surprisingly, DNAse I is commonly added enzyme to stimulate PADPRP activity in biological samples such as cell ghosts (2) and nuclei preparations (3). A high level of DNA-strand breaks helps to accurately determine the amount of PADPRP activity in these samples.

Keywords

Migration Albumin Lithium Electrophoresis Polyacrylamide 

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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Rafael Alvarez-Gonzalez
    • 1
  • Guy G. Poirier
    • 2
  • Marcos Martinez
    • 1
  1. 1.Department of Microbiology and Immunology, Texas College of Osteopathic MedicineThe University of North TexasFort WorthUSA
  2. 2.Department of Biochemistry, Faculty of MedicineUniversite Laval QuebecQuebecCanada

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