Proteolytic Degradation of Poly(ADP-ribose)polymerase by Contaminating Proteases in Commercial Preparations of DNAse I

  • Rafael Alvarez-Gonzalez
  • Guy G. Poirier
  • Marcos Martinez
Conference paper


The stimulation of poly(ADP-ribose)polymerase (PADPRP) activity by the addition of agents that induce DNA damage and formation of DNA strand-breaks in vitro and in vivo is well established (1). Not surprisingly, DNAse I is commonly added enzyme to stimulate PADPRP activity in biological samples such as cell ghosts (2) and nuclei preparations (3). A high level of DNA-strand breaks helps to accurately determine the amount of PADPRP activity in these samples.


Nuclear Matrix Proteolytic Degradation Commercial Preparation Cell Ghost Electrophoretic Migration 
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  1. 1.
    Althaus, F. R., and Richter, C. (1987) ADP-ribosylation of Proteins: Enzymology and Biological Significance. Molecular Biology, Biochemistry, and Biophysics (Springer Verlag Heidelberg New York Tokyo), Vol. 37Google Scholar
  2. 2.
    Benjamin, R. C., and Gill, D. M. (1980) J Biol Chem 255: 10493–10501PubMedGoogle Scholar
  3. 3.
    Cesarone, C. F., Scarabelli, L., Giannoni, P., and Oruneso, M. (1990) Mutation Res 245: 157–163PubMedCrossRefGoogle Scholar
  4. 4.
    Wesierska-Gadek and Sauermann, G. (1985) Eur J Biochem 153, 421–428PubMedCrossRefGoogle Scholar
  5. 5.
    Adolph, K. W., and Song, M.-K. H. (1983) Biochem Biophys Res Commun 115: 938–945PubMedCrossRefGoogle Scholar
  6. 6.
    Alvarez-Gonzalez, R. and Ringer, D. P. (1988) FEBS Letters 236: 362–366PubMedCrossRefGoogle Scholar
  7. 7.
    Cardenas-Corona, Ma.-E., Jacobson, E. L., and Jacobson, M. K. (1987) J Biol Chem 262, 5857–5865Google Scholar
  8. 8.
    Pedraza-Reyes, M. and Alvarez-Gonzalez, R. (1990) FEBS Letters 277: 88–92PubMedCrossRefGoogle Scholar
  9. 9.
    Berezney, R., and Buchholtz, L. A. (1981) Exp Cell Res 132: 1–13PubMedCrossRefGoogle Scholar
  10. 10.
    Panzeter, P. L., Etheredge, J. L. Kizer, D. E., and Ringer, D. P. (1987) Biochem Biophys Res Commun 149: 27–37PubMedCrossRefGoogle Scholar
  11. 11.
    Moy, B. C. and Tew, K. D. (1985) Chem Biol Interactions 54: 209–222CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Rafael Alvarez-Gonzalez
    • 1
  • Guy G. Poirier
    • 2
  • Marcos Martinez
    • 1
  1. 1.Department of Microbiology and Immunology, Texas College of Osteopathic MedicineThe University of North TexasFort WorthUSA
  2. 2.Department of Biochemistry, Faculty of MedicineUniversite Laval QuebecQuebecCanada

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