Proteolytic Degradation of Poly(ADP-ribose)polymerase by Contaminating Proteases in Commercial Preparations of DNAse I
The stimulation of poly(ADP-ribose)polymerase (PADPRP) activity by the addition of agents that induce DNA damage and formation of DNA strand-breaks in vitro and in vivo is well established (1). Not surprisingly, DNAse I is commonly added enzyme to stimulate PADPRP activity in biological samples such as cell ghosts (2) and nuclei preparations (3). A high level of DNA-strand breaks helps to accurately determine the amount of PADPRP activity in these samples.
KeywordsMigration Albumin Lithium Electrophoresis Polyacrylamide
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