Abstract
Solid phase fixed peptides are useful tools for the investigation of enzyme substrate interactions. We could show that interactions of target sequences, like e.g. the acceptor domain of G-proteins for the of mono ADP-ribosylation by pertussis toxin and the toxin itself can be studied by this method. Reaction specifity is identical to modification of free peptides. The method is extremly versatile and has enourmous potential if the focus is on the effects of substrate modification or substrate length.
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References
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© 1997 Springer Science+Business Media New York
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von Olleschik-Elbheim, L., el Bayâ, A., Alexander Schmidt, M. (1997). Membrane Anchored Synthetic Peptides as a Tool for Structure-Function Analysis of Pertussis Toxin and its Target Proteins. In: Haag, F., Koch-Nolte, F. (eds) ADP-Ribosylation in Animal Tissues. Advances in Experimental Medicine and Biology, vol 419. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8632-0_10
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DOI: https://doi.org/10.1007/978-1-4419-8632-0_10
Publisher Name: Springer, Boston, MA
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Online ISBN: 978-1-4419-8632-0
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