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Bovine Serum Albumine Complexation with Some Polyampholytes

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Abstract

Purified protein preparations, which recently were used only in biochemical laboratories, nowadays are coming more and more into our everyday life as medicine preparations and detergents. They are used in fine organic synthesis and food production as well as in a variety of analytical techniques. One of the effective methods of protein separation and purification is protein precipitation (liquid phase splitting) with the help of polyelectrolytes. The most extensively studied protein complexes are mixtures of lysozyme1, albumins2-4, gelatin5,6, and catalase7,8 with weak and strong polyelectrolytes of linear and crosslinked structure.

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References

  1. J. Y. Shieh and C. E. Glatz, Precipitation of proteins with polyelectrolytes: Role of polymer molecular weight, In: Macromolecular Complexes in Chemistry and Biology, edited by P. Dubin, J. Block, R. Davis, D. N. Schulz, and C. Thies, (Springer-Verlag, Berlin, 1995). pp. 273–284.

    Google Scholar 

  2. E. Kokufuta, Complexation of proteins with polyelectrolytes in a salt-free system and biochemical characteristics of the resulting complexes, In: Macromolecular Complexes in Chemistry and Biology, edited by P. Dubin, J. Block, R. Davis, D. N. Schulz, and C. Thies, (Springer-Verlag, Berlin, 1995). pp. 300–325.

    Google Scholar 

  3. K. W. Mattison, I. J. Brittain, and P. L. Dubin, Protein-polyelectrolyte phase boundary, Biotechnol. Prog. 11, 632–637 (1995).

    Article  CAS  Google Scholar 

  4. A. Tsuboi, T. Izumi, M. Hirata, J. Xia, P. Dubin, and E. Kokufuta, Complexation of proteins with a strong polyanion in an aqueous salt-free system, Langmuir 12, 6295–6303 (1996).

    Article  CAS  Google Scholar 

  5. J. Greener, B. A. Contestable, and M. D. Bale, Interaction of anionic surfactants with gelatin: Viscosity effects, Macromolecules 20, 2490–2498 (1987).

    Article  CAS  Google Scholar 

  6. . W. A. Bowman, M. Rubinstein, and J. S. Tan, Polyelectrolyte-gelatin complexation: Light-scattering study, Macromolecules 30, 3262–3270 (1997).

    Article  CAS  Google Scholar 

  7. G. A. Bektenova, E. A. Bekturov, and S.E. Kudaibergenov, Interaction of catalase with cationic hydrogels: Influence of pH, kinetics of process and isotherms of adsorption, Polym. Adv. Technol. 10, 141–145 (1999).

    Article  CAS  Google Scholar 

  8. G. A. Bektenova, E. A. Bekturov, G. K. Sulekeshova, and S. E. Kudaibergenov, Interaction of amphoteric hydrogels with catalase: Influence of pH and ionic strength, Polym. Prepr. 41(1), 750–751 (2000).

    CAS  Google Scholar 

  9. J. Xia and P. L. Dubin, Protein-polyelectrolyte complexes, In: Macromolecular Complexes in Chemistry and Biology, edited by P. Dubin, J. Block, R. Davis, D. N. Schulz, and C. Thies, (Springer-Verlag, Berlin, 1995). pp. 247–271.

    Google Scholar 

  10. C. S. Patrickios, C. J. Jang, W. R. Hertler, and T. A. Hatton, Protein interactions with acrylic polyampholytes, Polym. Prepr. 34(1), 954–955 (1993).

    CAS  Google Scholar 

  11. C. S. Patrickios, C. J. Jang, W. R. Hertler, and T. A. Hatton, Protein interactions with acrylic polyampholytes, in: Macro-ion Characterization from Dilute Solutions to Complex Fluids, edited by K. S. Schmitz, (ACS Symposium Series, Washington DC, v. 548, Ch.19, p. 257–267 1994).

    Google Scholar 

  12. C. S. Patrickios, L. R. Sharma, S. P. Armes, and N. C. Billingham, Precipitation of a water-soluble ABC triblock methacrylic polyampholyte. Effects of time, polymer concentration, salt type and concentration, and presence of a protein, Langmuir 15, 1613–1620 (1999).

    Article  CAS  Google Scholar 

  13. S. Nath, C. S. Patrickios, and T. A. Hatton, A turbidimetric titration study of the interaction of proteins with block and random acrylic polyampholytes, Biotechnol.Prog. 11(1), 99–103 (1995).

    Article  CAS  Google Scholar 

  14. S. Nath, Complexation behavior of proteins with polyelectrolytes and random acrylic polyampholytes using turbidimetric titration, J. Chem. Tech. Biotech. 62(3), 295–300 (1995).

    Article  CAS  Google Scholar 

  15. C. S. Patrickios, W. R. Hertler, and T. A. Hatton, Protein complexation with acrylic polyampholytes, Biotechnol. Bioeng. 44, 1031–1039 (1994).

    Article  CAS  Google Scholar 

  16. S. E. Kudaibergenov and E. A. Bekturov, Influence of the coil-globule confromational transition in polyampholytes on sorption and desorption of polyelectrolytes and human serum albumin, Vysokomol. Soedin. Ser.A. 31, 2614–2617 (1989).

    CAS  Google Scholar 

  17. A. K. Tultaev, S. E. Kudaibergenov, and E. A. Bekturov, Study of complexation of synthetic polyampholyte with human serum albumin, Izv. Akad. Nauk KazSSR, Ser. Khim. 6, 67–71 (1990).

    Google Scholar 

  18. H. Morawetz and W. L. Hughes, The interaction of proteins with synthetic polyelectrolytes. 1. Complexing of bovine serum albumin, J. Phys. Chem. 56, 64–69 (1952).

    Article  CAS  Google Scholar 

  19. L. S. Rodkey and A. Hirata, Studies of ampholyte-protein interactions, Prot. Biol. Fluides, 34, 745–748 (1986).

    CAS  Google Scholar 

  20. O. Sh. Kurmanaliev, E. M. Shaikhutdinov, Sh. S. Tulbaev, and T. M. Mukhametkaliev, Influence of water on radical copolymerization of 1,2,5-trimethyl-4-vinylethynylpiperidinol-4 with methacrylic acid, Vysokomolek. Soedin. Ser B, 22, 526–528 (1980).

    CAS  Google Scholar 

  21. S. E. Kudaibergenov, Synthesis and characterization of Schiff base polyampholytes (to be published).

    Google Scholar 

  22. R. C. Schulz, M. Schmidt, E. Schwarzenbach, J. Zoller, Some new polyelectrolytes, Macromol.Chem., Macromol. Symp. 26, 221–231 (1989).

    Article  CAS  Google Scholar 

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Didukh, A.G., Makysh, G.S., Bimendina, L.A., Kudaibergenov, S.E. (2003). Bovine Serum Albumine Complexation with Some Polyampholytes. In: Geckeler, K.E. (eds) Advanced Macromolecular and Supramolecular Materials and Processes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8495-1_20

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  • DOI: https://doi.org/10.1007/978-1-4419-8495-1_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4630-2

  • Online ISBN: 978-1-4419-8495-1

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