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Solid State Nanopores for Selective Sensing of DNA

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Nanopores

Abstract

This chapter focuses on the functionalized solid state nanopores for the purpose of rapidly and accurately sensing specific sequence of DNA. Fabrication processes are described, consisting of standard photolithography followed by using either a transmission electron microscope or a plasma polymer film to create and shrink the nanopore. The molecular dynamics of DNA-nanopore interactions are also discussed. Smaller pore diameter results in slower translocation of DNA through the nanopore, but increases van der waals force on the DNA and decreases the ionic current. Increase in applied voltage decreases the van der Waals force while increasing the ionic current and translocation velocity. Chemical functionalization of nanopores is then discussed. This allows a nanopore to be selective with translocating specific DNA sequence. This is done by modifying the surface in an attempt to control its surface charges and hydrophobicity. Probe DNA is used to functionalize the pore and achieve selectivity. In terms of sensing, perfect complementary DNA translocates faster than single-base-mismatch DNA. The flux can be measured from the current pulses when the translocating DNA blocks the nanopore under applied voltage.

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Author information

Authors and Affiliations

  1. Department of Electrical Engineering, Nanotechnology Research and Teaching Facility, Joint Graduate Studies Committee of the Bioengineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, TX, 76019, Austin, USA

    Samir M. Iqbal

Authors
  1. Waseem Asghar
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  2. Joseph A. Billo
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  3. Samir M. Iqbal
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Correspondence to Samir M. Iqbal .

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Editors and Affiliations

  1. Department of Electrical Engineering, Nanotechnology Research and Teaching Fac, University of Texas at Arlington, S. Cooper Street, Arlington, 76019-0119, Texas, USA

    Samir M. Iqbal

  2. Department of Electrical and Computer En, University of Illinois at Urbana-Champai, N. Wright Street 208, Urbana, 61820, Illinois, USA

    Rashid Bashir

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Asghar, W., Billo, J.A., Iqbal, S.M. (2011). Solid State Nanopores for Selective Sensing of DNA. In: Iqbal, S., Bashir, R. (eds) Nanopores. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-8252-0_5

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  • DOI: https://doi.org/10.1007/978-1-4419-8252-0_5

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