Dako has been an industry leader developing automated staining instruments for decades. We have invited Dako to offer perspectives and insights on the past, present, and future of automated staining systems. Experts from Dako answer questions regarding what automation can and cannot provide, how automation can improve workflow and secure high staining quality, gains from connection to laboratory information systems, automated in situ hybridization assays, and the next steps in automation of staining and other processes in the pathology laboratory.
This is a preview of subscription content, log in to check access.
Moreau A, Neel TE, Joubert M. Approach to automation in immunohistochemistry. J Clin Chim Acta. 1998;278:177–84.CrossRefGoogle Scholar
Boenisch T. Effect of heat-induced antigen retrieval following inconsistent formalin fixation. Appl Immunol Mol Morphol. 2005;13(3):283–6.CrossRefGoogle Scholar
Shi S-R, Liu C, Taylor CR. Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen-retrieval technique: from experiments to hypothesis. J Histochem Cytochem. 2007;55(2):105–9.PubMedCrossRefGoogle Scholar
Wolff AC, Hammond ME, Schwartz JN, et al. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. J Clin Oncol. 2007;25(1):118–45.PubMedCrossRefGoogle Scholar
García-Caballero T, Grabau D, Green AR, et al. Determination of HER2 amplification in primary breast cancer using dual-color chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicenter study involving 168 specimens. Histopathology. 2010;56(4):472–80.PubMedCrossRefGoogle Scholar
Hoff K, Jørgensen JT, Müller S, et al. Visualization of FISH Probes by dual-color chromogenic in situ hybridization. Am J Clin Pathol. 2009;133(2):205–11.CrossRefGoogle Scholar
Pedersen M, Rasmussen BB. The correlation between dual-color chromogenic in situ hybridization and fluorescence in situ hybridization in assessing HER2 gene amplification in breast cancer. Diagn Mol Pathol. 2009;18(2):96–102.PubMedCrossRefGoogle Scholar
Tanner M, Gancberg D, Di Leo A, et al. Chromogenic in situ hybridization: a practical alternative for fluorescence in situ hybridization to detect HER-2/neu oncogene amplification in archival breast cancer samples. Am J Pathol. 2000;157(5):1467–72.PubMedCrossRefGoogle Scholar