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Molecular Strategies: Detection of Foodborne Bacterial Pathogens

  • Javed Ahamad Khan
  • R. S. Rathore
  • Iqbal Ahmad
  • Shaheen Khan
Chapter

Abstract

Conventional methods of pathogen identification have often depended on the identification of disease symptoms, isolation, and culturing of the organisms, and identification by morphology and biochemical tests. The major limitations of these culture-based morphological approaches, however, are the reliance on the ability of the organism to be cultured, the time-consuming nature, and requirement of extensive taxonomic expertise. The use of molecular methods can circumvent many of these shortcomings. Accordingly, there have been significant developments in the area of molecular detection of bacterial pathogens in the last 3 decades. We report here a brief overview of the molecular detection methods applicable to microbes from food.

Keywords

Polymerase Chain Reaction Amplify Fragment Length Polymorphism Multiplex Polymerase Chain Reaction Foodborne Pathogen Amplify Fragment Length Polymorphism Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Javed Ahamad Khan
    • 1
    • 2
  • R. S. Rathore
  • Iqbal Ahmad
  • Shaheen Khan
  1. 1.Division of ImmunologyIndian Veterinary Research InstituteBareillyIndia
  2. 2.Department of Agricultural MicrobiologyAligarh Muslim UniversityAligarhIndia

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