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Future Application of Probiotics: A Boon from Dairy Biology

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Microbes and Microbial Technology

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Abstract

Microbes have been used for millenia in food and alcoholic ­fermentations; in recent years, microbes have undergone scientific scrutiny of their ability for preventive and therapeutic effects in humans. This work has led to the establishment of a new term, “probiotics.” Lactic acid bacteria (LAB) are normal microflora of the intestine of most animals. They play an important role in humans and other animals and act as an immunomodulator. LAB are helpful in disease treatment and prevention, as well as for improved digestion and absorption of nutrients. Probiotic microorganisms include LAB i.e., Lactobacillus acidophilus, L. bulgaricus, L. casei, L. plantarum, L. rhamnosus, etc. Use of these live bacteria to elicit an immune response or to carry a vaccine component is a new development in vaccine formulation. The advantages of live bacterial vaccines are their ability to mimic the natural infection, their intrinsic adjuvant properties, and that they can be administered orally. Components of pathogenic and nonpathogenic food-related microbes are currently being evaluated as candidates for oral vaccines.

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References

  • Akira, S., Takeda, K. and Kaisho, T. (2001) Toll-like receptors: critical proteins linking innate and acquired immunity. Nat. Immunol. 2: 675–680.

    Article  CAS  Google Scholar 

  • Aldovini, A. and Young, R. A. (1991) Humoral and cell-mediated immune response to live recombinant BCG-HIV vaccines. Nature 351: 479–482.

    Article  CAS  Google Scholar 

  • Anderson, E. J. and Weber, S. G. (2004) Rotavirus infection in adults. Lancet Infect. Dis. 4(2): 91–99.

    Article  Google Scholar 

  • Benyacoub, J., Hopkins, S., Potts, A., Kelly, S., Kraehenbuhl, J. P., Curtiss, R. III, De Grandi, P. and Nardelli-Haefliger, D. (1999) The nature of the attenuation of Salmonella typhimurium strains expressing human papillomavirus type 16 virus-like particles determines the systemic and mucosal antibody responses in nasally immunized mice. Infect. Immun. 67: 3674–3679.

    CAS  Google Scholar 

  • Bernet, M. F., Brassart, D., Neeser, J. R. and Servin, A. L. (1994).Lactobacillus acidophilus LA1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut 35: 483–489.

    Article  CAS  Google Scholar 

  • Blackman, M. J., Ling, I. T., Nicholls, S. C. and Holder, A. A. (1991) Proteolytic processing of the Plasmodium falciparum merozoite surface protein-1 produces a membrane-bound fragment containing two epidermal growth factor-like domains. Mol. Biochem. Parasitol. 49: 29–33.

    Article  CAS  Google Scholar 

  • Casas, I. A. and Dobrogosz, W. J. (1997) Lactobacillus reuteri: overview of a new probiotic for humans and animals. Microecol. Ther. 26: 221–231.

    Google Scholar 

  • Chukeatirote, E. (2003) Potential use of probiotics Songklanakarin. J. Sci. Technol. 25(2): 275–282.

    Google Scholar 

  • Classen, E., Van Vinsen, R., Posko, M. and Boersma, W. A. U. (1995) New and safe “oral” live vaccines based on Lactobacillus. Adv. Exp. Med. Biol. 371B: 1553–1558.

    Google Scholar 

  • Detme, A. and Glenting, J. (2006) Live bacterial vaccines – A review and identification of potential hazards. Microb. Cell Fact. 5: 23.

    Article  Google Scholar 

  • Dietrich, G., Griot-Wenk, M., Metcalfe, I. C., Lang, A. B. and Viret, J. F. (2003) Experience with registered mucosal vaccines. Vaccine 21: 678–683.

    Article  CAS  Google Scholar 

  • Donohue, D. C. and Salminen, S. (1996) Safety of probiotic bacteria. Asia Pac. J. Clin. Nutr. 5: 25–28.

    Google Scholar 

  • Elmer, G. W. (2001) Probiotics: “living drugs.” Am. J. Health Syst. Pharm. 58: 1101–1109.

    CAS  Google Scholar 

  • Fuller, R. (1989) Probiotics in man and animals. J. Appl. Bacteriol. 66: 365–378.

    CAS  Google Scholar 

  • Fuller, R. (1991) Probiotics in human medicine. Gut 32: 439–442.

    Article  CAS  Google Scholar 

  • Goldin, B. R. Gorbach, S. L., Saxelin, M., Barakat, S., Gualtieri, L. and Salminen, S. (1992) Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig. Dis. Sci. 37: 121–128.

    Article  CAS  Google Scholar 

  • Gomboova, A., Deme, P. and Valent, M. (1986) Immunotherapeutic effect of the lactobacillus vaccine, Solco Trichovac, in trichomoniasis is not mediated by antibodies cross reacting with Trichomonas vaginalis. Genitourin. Med. 62: 107–110.

    Google Scholar 

  • Gonzalez, S. N., Cardoza, R., Apella, M. C. and Oliver, G. (1995) Biotherapeutic role of ­fermented milk. Biotherapy 8: 129–134.

    Article  Google Scholar 

  • Goodwin, J. S. (1995) Decreased immunity and increased morbidity in the elderly. Nutr. Rev. 53:S41–S46.

    Article  CAS  Google Scholar 

  • Greene, J. D. and Klaenhammer, T. R. (1994) Factors involved in adherence of Lactobacilli to human Caco-2 cells. Appl. Environ. Microbiol. 60: 4487–4494.

    CAS  Google Scholar 

  • Havenaar, R., Brink, B. T. and Huisin’t Veld, J. H. J. (1992) Selection of strains for probiotic use. In: Probiotics, the Scientific Basis. Fuller R., ed. Chapman and Hall, London. 209–224

    Google Scholar 

  • Holder, A. A. and Freeman, R. R. (1981) Immunization against blood-stage rodent malaria using purified parasite antigens. Nature 294: 361–364.

    Article  CAS  Google Scholar 

  • Holzapfel, W. H., Haberer, P., Snel, J., Schillinger, U. and HuisIn’t Veld, J. H. (1998) Overview of gut flora and probiotics. Int. J. Food Microbiol. 41: 85–101.

    Article  CAS  Google Scholar 

  • Isolauri, E., Juntunen, M., Rautanen, T., Sillanaukee, P. and Koivula, T. (1991) A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Pediatrics 88: 90–97.

    CAS  Google Scholar 

  • Jack, R. W., Tagg, J. R. and Ray, B. (1994) Bacteriocins of Grampositive bacteria. Microbiol. Rev. 59: 171–200.

    Google Scholar 

  • Kajikawa, A. E., Satoh, R. J., Leer, S., Yamamoto, S. and Igimi, S. (2007) Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis. Vaccine 25: 3599–3605.

    Article  CAS  Google Scholar 

  • Ke-Qin, X., Yuka, H., Yoshihiko, T., Shizunobu, I., Yoshitsugu, K., Nao, J., Kenji, O., Akira, K., Mayumi, K., Kenji, H., Dennis, K. and Kenji, O. (2003) Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV Env. Blood 102: 223–229.

    Article  Google Scholar 

  • Khansari, D. N., Murgo, A. J. and Faith, A. E. (1990) Effects of stress on the immune system. Immunol. Today 11: 170–175.

    Article  CAS  Google Scholar 

  • Kirjavainen, P. E., Ouwehand, A. C., Isolauri, E. and Salminen, S. J. (1998) The ability of probiotic bacteria to bind to human intestinal mucus. FEMS Microbiol. Lett. 167: 185–189.

    Article  CAS  Google Scholar 

  • Klijn, N., Weerkamp, A. H. and de Vos, W. M. (1995) Genetic marking of Lactococcus lactis shows its survival in the human gastrointestinal tract. Appl. Environ. Microbiol. 61: 2771–2774.

    CAS  Google Scholar 

  • Kochi, S. K., Killeen, K. P. and Ryan, U. S. (2003) Advances in the development of bacterial vector technology. Expert. Rev. Vaccines 74(2): 31–43.

    Google Scholar 

  • Korik, L. M., Ljubimova, L. K. and Tovstolec, K. P. (1968) Immediate and remote results of treatment of trichomonosis in men with trichomonal vaccine. Vestn. Dermatol. Venerol. 42: 80–84.

    CAS  Google Scholar 

  • Kotloff, K. L., Noriega, F., Losonsky, G. A., Sztein, M. B., Wasserman, S. S., Nataro, J. P. and Levine, M. M. (1996) Safety, immunogenicity, and transmissibility in humans of CVD 1203, a live oral Shigella flexneri 2a vaccine candidate attenuated by deletions in aroA and virG. Infect. Immun. 64: 4542–4548.

    CAS  Google Scholar 

  • Kukreja, R. C., Kontos, M. C. and Hess, M. L. (1996) Free radicals and heat shock protein in the heart. Ann. NY Acad. Sci. 793: 108–122.

    Article  CAS  Google Scholar 

  • Lin, S. Y. and Chen, C. T. (2000) Reduction of cholesterol by Lactobacillus acidophilus in culture broth. J. Food Drug Anal. 8: 97–102.

    CAS  Google Scholar 

  • Lin, S. Y., Ayres, J. W., Winkler, W. and Sandine, W. E. (1989) Lactobacillus effects on cholesterol: in vitro and in vivo results. J. Dairy Sci. 72: 2885–2899.

    Article  CAS  Google Scholar 

  • Lindberg, A. A. (1995) The history of live bacterial vaccines. Dev. Biol. Stand. 84: 211–219.

    CAS  Google Scholar 

  • Lindberg, A. A. (1998) Vaccination against enteric pathogens: from science to vaccine trials. Curr. Opin. Microbiol. 1: 116–124.

    Article  CAS  Google Scholar 

  • Link-Amster, H., Rochat, F., Saudan, K., Mignot, O. and Aeschlimann, J. (1994) Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented milk. FEMS Immunol. Med. Microbiol. 10: 55–63.

    Article  CAS  Google Scholar 

  • Matsuzak, T. and Chin, J. (2000) Modulating immune response with probiotic bacteria. Immunol. Cell Biol. 78: 67–73.

    Article  Google Scholar 

  • Mattingly, J. and Waksman, B. (1978) Immunologic suppression after oral administration of antigen I Specific suppressor cells formed in rat Peyer’s patches after oral administration of sheep erythrocytes and their systemic migration. J. Immunol. 121: 1878–1883.

    CAS  Google Scholar 

  • McGhee, J. R., Mestecky, J., Dertzbaugh, M. T., Eldridge, J. H., Hirasawa, M. and Kiyono, H. (1992) The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10: 75–88.

    Article  CAS  Google Scholar 

  • Mercenier, A., Muller-Alouf, H. and Grangette, C. (2000) Lactic acid bacteria as live vaccines. Curr. Issues Mol. Biol. 2: 17–25.

    CAS  Google Scholar 

  • Metchnikoff, E. (1908) The prolongation of life. New York, NY: CP Putnam’s Sons. 151–183.

    Google Scholar 

  • Miller, L. H., Roberts, T., Shahabuddin, M. and McCutchan, T. F. (1993) Analysis of sequence diversity in the Plasmodium falciparum merozoite surface protein-1 (MSP-1). Mol. Biochem. Parasitol. 59: 1–14

    Article  CAS  Google Scholar 

  • Morimoto, R. I. and Santoro, M. G. (1998) Stress-inducible responses and heat shock proteins: new pharmacologic targets for cytoprotection. Nat. Biotechnol. 16: 833–838.

    Article  CAS  Google Scholar 

  • Nardelli-Haefliger, D., Kraehenbuhl, J. P., Curtiss, R. III, Schodel, F., Potts, A., Kelly, S. and De Grandi, P. (1996) Oral and rectal immunization of adult female volunteers with a recombinant attenuated Salmonella typhi vaccine strain. Infect. Immun. 64: 5219–5224.

    CAS  Google Scholar 

  • Netherwood, T., Gilbert, H. J., Parker, D. S. and O’Donnell, A. G. (1999) Probiotics shown to change bacterial community structure in the avian gastrointestinal tract. Appl. Environ. Microbiol. 65: 5134–5138.

    CAS  Google Scholar 

  • Neutra, M. and Krahenbuhl, J. (1996) M cells as a Pathway for antigen uptake and processing. In: Essentials of Mucosal Immunology. Kagnoff M. and Kiyono H., eds. Academic, New York, NY. 2: 29–36.

    Chapter  Google Scholar 

  • Ouwehand, A. C., Kirjavainen, P. V., Gronlund, M. M., Isolauri, E. and Salminen, S. (1999) Adhesion of probiotic micro-organisms to intestinal mucus. Int. Dairy J 9: 623–630.

    Article  Google Scholar 

  • Pawelec, G., Adibzadeh, M., Pohla, H. and Schaudt, K. (1995) Immunosenescence: aging of the immune system. Immunol. Today 16: 420–422.

    Article  CAS  Google Scholar 

  • Perdigón, G. and Alvarez, S. (1992) Probiotics and the immune state. In: Probiotics. The Scientific Basis. Fuller R., ed. Chapman and Hall, London. 7: 145–180.

    Google Scholar 

  • Phillips-Quagliata, J. and Lamm, M. (1988) Migration of lymphocytes in the mucosal immune system. In: Migration and Homing of Lymphoid Cells. Husband A., ed. CRC Press, Boca Raton, FL. 53–75.

    Google Scholar 

  • Reid, G. (1999) The scientific basis for probiotic strains of Lactobacillus. Appl. Environ. Microbiol. 65: 3763–3766.

    CAS  Google Scholar 

  • Reid, G. and Burton, J. (2002) Use of Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect. 4: 319–324.

    Article  Google Scholar 

  • Reid, G., Servin, A., Bruce, A. W. and Busscher, H. J. (1993) Adhesion of three Lactobacillus strains to human urinary and intestinal epithelial cells. Songklanakarin J. Sci. Technol. Microbios. 75: 57–65.

    CAS  Google Scholar 

  • Salimen, S., Isolauri, E. and Salimen, E. (1996) Probiotics for stabilising the gut mucosal barrier: successful strains and future challenges. Antonie van Leeuwenhoek 70: 347–358.

    Article  Google Scholar 

  • Sarem-Damerdjii, L. O., Sarem, F., Marchal, L. and Nicolas, J. P. (1995) In vitro colonisation ability of human colon mucosa by exogenous Lactobacillus strains. FEMS Micorbiol. Lett. 131: 133–137.

    Article  Google Scholar 

  • Schnell, M. J., Foley, H. D., Siler, C. A., Mc Gettigan, J. P., Dietzachold, B. and Pomerantz, R. J. (2000) Recombinant rabies virus as potential live-viral vaccines for HIV-1. Proc. Natl. Acad. Sci. USA 97: 3544–3549.

    Article  CAS  Google Scholar 

  • Shornikova, A. V., Casas, A., Mykkanen, H., Salo, E. and Vesikari (1997) Bacteriotherpy with Lactobacillus reuteri in rotavirus gastroenteritis. Pediatr. Infect. Dis. J. 16: 1103–1107.

    Article  CAS  Google Scholar 

  • Singh, V., Singh, K., Amdekar, S., Singh, D. D., Tripathi, P., Sharma, G. L. and Yadav, H. (2008) Innate and specific gut-associated immunity and microbial interference. FEMS Immunol. Med. Microbiol. 55: 6–12.

    Article  Google Scholar 

  • Strobel, S. (1995) Oral tolerance. In: Mucosal Immunity and the Gut Epithelium: Interactions in Health and Disease. Dyn Nutr Res. Karger, Basel. 4: 65–75.

    Google Scholar 

  • Tahri, K., Grill, J. P. and Schneider, F. (1996) Bifidobacteria strain behavior toward cholesterol: Coprecipitation with bile salts and assimilation. Curr. Microbiol. 33: 187–193.

    Article  CAS  Google Scholar 

  • Tatyana, O., Michal, S., Adriana, D., Klein, R., Natalie, A. and Babeth, R. (2000) A new method of long-term preventive cardioprotection using Lactobacillus. Am. J. Physiol. Heart Circ. Physiol. 278: 1717–1724.

    Google Scholar 

  • Underdahl, H., Medina, A. and Doster, A. (1982) Effect of Streptococcus faecium C-68 in control of Escherichia coli induced diarrhea in gnotobiotic pigs. Am. J. Vet. Res. 43: 2227–2232.

    CAS  Google Scholar 

  • Velazquez, F. R., Matson, D. O., Calva, J. J., Guerrero, S. L., Morrow, A. L., Carter-Campbell, S., Glass, R. I., Estes, M. K., Pickering, L. K. and Ruiz-Palacios. (1996) Rotavirus infections in infants as protection against subsequent infections. N. Eng. J. Med. 335(14): 1022–1028.

    Article  CAS  Google Scholar 

  • Weissman, D., Ni, H., Scales, D., Dude, A., Capodici, J., Mcgibney, K. Abdool., Isaacs, S., Cannon, G. and Kariko, K. (2000) HIV gag mRNA transfection of dendritic cells (DC) delivers encoded antigen to MHC class I and II molecules, causes DC maturation, and induces a potent human in vitro primary immune response. J. Immunol. 165: 4710–4717.

    CAS  Google Scholar 

  • Woodward B (1998) Protein, calories and immune defenses. Nutr. Rev. 56: S84–S92.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Barkatullah University, 462026, Bhopal, Madhya Pradesh, India

    Vinod Singh

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  1. Vinod Singh
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  2. Sarika Amdekar
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  3. Hariom Yadav
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  4. Nagendra Nath Mishra
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  5. Shailini Jain
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Correspondence to Vinod Singh .

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Editors and Affiliations

  1. Fac. Agricultural Sciences, Dept. Agricultural Microbiology, Aligarh Muslim University, Aligarh, 202002, India

    Iqbal Ahmad

  2. of Biomedical Sciences and Research, Department of Microbiology, Sardan Bhagwan Singh Post Graduate Insti, Dehradun, Uttaranchal, India

    Farah Ahmad

  3. , Natural Resources and Environmental Mana, Ball State University, Muncie, 47306, Indiana, USA

    John Pichtel

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Singh, V., Amdekar, S., Yadav, H., Mishra, N.N., Jain, S. (2011). Future Application of Probiotics: A Boon from Dairy Biology. In: Ahmad, I., Ahmad, F., Pichtel, J. (eds) Microbes and Microbial Technology. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7931-5_4

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