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The Molecular Immunology of Complex Carbohydrates-3 pp 397–410Cite as

Roles for N- and O-Glycans in Early Mouse Development

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 705))

Abstract

Glycosylation is the most abundant posttranslational protein modification. Specific glycans covalently attached to glycoproteins contribute to their functions, ensuring appropriate folding, secretion, half-life, and receptor–ligand interactions [1]. Many different classes of glycans exist, but those discussed herein are the complex and hybrid N-glycans, core 1-derived O-glycans, and O-linked fucose glycans. The synthesis of each class of glycan is initiated by the addition of a single sugar, or group of sugars, to certain amino acids or amino acid sequons by specific glycosyltransferases via a particular linkage. The subsequent sugars are added individually in a carefully orchestrated pathway by specific glycosyltransferases that reside in the secretory compartments of the cell. Thus, the glycans ultimately synthesized by a cell depend on the cohort of glycosyltransferases, nucleotide sugar synthases, and transporters expressed by that cell, which will be influenced by metabolic state and stage of development. To determine roles for complex and hybrid N-glycans, core 1-derived O-glycans, and O-fucose glycans (Fig. 20.1) in oogenesis, fertilization, blastogenesis, implantation, and embryonic development, we used a maternal and zygotic gene-targeting approach.

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Author information

Authors and Affiliations

  1. Department of Cell Biology, Albert Einstein College of Medicine, New York, NY, 1046, USA

    Pamela Stanley

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  1. Suzannah A. Williams
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  2. Pamela Stanley
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Correspondence to Pamela Stanley .

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Editors and Affiliations

  1. College of Medicine, Institute of Molecular&Cellular Biology, Chang-Gung University, Kwei-San, Tao-Yuan, 333, Taiwan R.O.C.

    Albert M. Wu

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Williams, S.A., Stanley, P. (2011). Roles for N- and O-Glycans in Early Mouse Development. In: Wu, A. (eds) The Molecular Immunology of Complex Carbohydrates-3. Advances in Experimental Medicine and Biology, vol 705. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7877-6_20

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