Post-Translationally Modified Proteins: Glycosylation and Disulfide Bond Formation

  • Anthony Tsarbopoulos
  • Fotini N. Bazoti


Even though most medicines have historically been small molecules, many newly approved drugs over the last two decades have been derived from proteins. For the past few years, protein therapeutics have been enjoying the fastest growth within the global pharmaceutical industry.


Glycan Structure Disulfide Linkage MALDI Mass Spectrometry Glycan Moiety Lectin Affinity Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Anion-Exchange Chromatography


β-Elimination followed by Michael Addition with Dithiothreitol


Capillary Electrophoresis


Chinese Hamster Ovary


CHO-derived Interleukin-4


Congenital Disorders of Glycosylation


Carbohydrate-Deficient Transferrin


Collision-Induced Dissociation


Concanavalin A


Consortium for Functional Glycomics


Capillary Zone Electrophoresis


cAMP-responsive Element-Binding Factor


2,5-Dihydroxybenzoic acid


Electron-Capture Dissociation


Electrospray Ionization


Electron-Transfer Dissociation


Fast Atom Bombardment


Fourier Transform Ion Cyclotron Resonance


Graphitized Carbon Columns


Hydrophilic Interaction Chromatography


3-hydroxypicolinic acid


High-Performance Liquid Chromatography


Human Papillomavirus


Horseradish Peroxidase




Interleukin-4 receptor


Interleukin-5 receptor α-subunit




Ion Mobility


Ion Trap


Kyoto Encyclopedia of Genes and Genomes


Lectin Affinity Chromatography


Liquid Chromatography


Liquid Chromatography–Mass Spectrometry


Liquid Secondary Ion


Monoclonal Antibodies


Matrix-Assisted Laser Desorption/Ionization


Multi-Lectin Affinity Chromatography


Molecular Weight


Mass Spectrometry


Tandem Mass Spectrometry


Mass-to-charge ratio


Nano-Electrospray Ionization


Nuclear Magnetic Resonance


Orthogonal Time-of-Flight




Plasma Desorption


Porous Graphitized Carbon Chromatography


Pisum Sativum Agglutinin


Post-Source Decay


Post-Translational Modifications


Recombinant Human Erythropoietin


Recombinant human Chorionic Gonadotrophin


Recombinant Tissue Plasminogen Activator


Quadrupole Time-of-Flight


Secreted Amyloid Precursor Protein


2,5-Dihydroxybenzoic acid (DHB) with a 10 % admixture of 2-hydroxy-5-methoxybenzoic acid (super DHB)


Size-Exclusion Chromatography


Selected Ion Monitoring


Serial Affinity Chromatography


Solid-Phase Extraction




Human Transferrin Glycoprotein




Urokinase-type Plasminogen Activator Receptor


Ultra-Performance Liquid Chromatography


Wheat Germ Agglutinin



We acknowledge the kind permission of the Schering-Plough Research Institute to reproduce previously reported, but unpublished data regarding the IL-5Rα.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of PharmacologyUniversity of Athens Medical SchoolAthensGreece
  2. 2.Bioanalytical DepartmentThe Goulandris Natural History MuseumKifissiaGreece

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