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Post-Translationally Modified Proteins: Glycosylation and Disulfide Bond Formation

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Book cover Characterization of Protein Therapeutics using Mass Spectrometry

Abstract

Even though most medicines have historically been small molecules, many newly approved drugs over the last two decades have been derived from proteins. For the past few years, protein therapeutics have been enjoying the fastest growth within the global pharmaceutical industry.

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Abbreviations

AEC:

Anion-Exchange Chromatography

BEMAD:

β-Elimination followed by Michael Addition with Dithiothreitol

CE:

Capillary Electrophoresis

CHO:

Chinese Hamster Ovary

CHO IL-4:

CHO-derived Interleukin-4

CDG:

Congenital Disorders of Glycosylation

CDT:

Carbohydrate-Deficient Transferrin

CID:

Collision-Induced Dissociation

ConA:

Concanavalin A

CFG:

Consortium for Functional Glycomics

CZE:

Capillary Zone Electrophoresis

CREB:

cAMP-responsive Element-Binding Factor

DHB:

2,5-Dihydroxybenzoic acid

ECD:

Electron-Capture Dissociation

ESI:

Electrospray Ionization

ETD:

Electron-Transfer Dissociation

FAB:

Fast Atom Bombardment

FT ICR:

Fourier Transform Ion Cyclotron Resonance

GCC:

Graphitized Carbon Columns

HILIC:

Hydrophilic Interaction Chromatography

HPA:

3-hydroxypicolinic acid

HPLC:

High-Performance Liquid Chromatography

HPV:

Human Papillomavirus

HRP:

Horseradish Peroxidase

IL-4:

Interleukin-4

IL-4R:

Interleukin-4 receptor

IL-5Rα:

Interleukin-5 receptor α-subunit

IA:

Immunoaffinity

IM:

Ion Mobility

IT:

Ion Trap

KEGG:

Kyoto Encyclopedia of Genes and Genomes

LAC:

Lectin Affinity Chromatography

LC:

Liquid Chromatography

LC–MS:

Liquid Chromatography–Mass Spectrometry

LSI:

Liquid Secondary Ion

mAb:

Monoclonal Antibodies

MALDI:

Matrix-Assisted Laser Desorption/Ionization

M-LAC:

Multi-Lectin Affinity Chromatography

Mr :

Molecular Weight

MS:

Mass Spectrometry

MS/MS:

Tandem Mass Spectrometry

m/z:

Mass-to-charge ratio

nESI:

Nano-Electrospray Ionization

NMR:

Nuclear Magnetic Resonance

oTOF:

Orthogonal Time-of-Flight

PAS:

Periodate-acid-Schiff

PD:

Plasma Desorption

PGC:

Porous Graphitized Carbon Chromatography

PSA:

Pisum Sativum Agglutinin

PSD:

Post-Source Decay

PTMs:

Post-Translational Modifications

rHuEPO:

Recombinant Human Erythropoietin

r-RhCG:

Recombinant human Chorionic Gonadotrophin

rtPA:

Recombinant Tissue Plasminogen Activator

QTOF:

Quadrupole Time-of-Flight

sAPP:

Secreted Amyloid Precursor Protein

sDHB:

2,5-Dihydroxybenzoic acid (DHB) with a 10 % admixture of 2-hydroxy-5-methoxybenzoic acid (super DHB)

SEC:

Size-Exclusion Chromatography

SIM:

Selected Ion Monitoring

SLAC:

Serial Affinity Chromatography

SPE:

Solid-Phase Extraction

TAS:

Tagging-via-Substrate

Tf:

Human Transferrin Glycoprotein

TOF:

Time-of-Flight

uPAR:

Urokinase-type Plasminogen Activator Receptor

UPLC:

Ultra-Performance Liquid Chromatography

WGA:

Wheat Germ Agglutinin

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Acknowledgments

We acknowledge the kind permission of the Schering-Plough Research Institute to reproduce previously reported, but unpublished data regarding the IL-5Rα.

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  1. Department of Pharmacology, University of Athens Medical School, 75 M. Asias street, Athens, 115 27, Greece

    Anthony Tsarbopoulos

  2. Bioanalytical Department, The Goulandris Natural History Museum, 13 Levidou street, Kifissia, 145 62, Greece

    Fotini N. Bazoti

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Correspondence to Anthony Tsarbopoulos .

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    Guodong Chen

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Tsarbopoulos, A., Bazoti, F.N. (2013). Post-Translationally Modified Proteins: Glycosylation and Disulfide Bond Formation. In: Chen, G. (eds) Characterization of Protein Therapeutics using Mass Spectrometry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7862-2_4

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