Scaffolding for Three-Dimensional Embryonic Vasculogenesis

  • Thomas P. Kraehenbuehl
  • Sezin Aday
  • Lino S. Ferreira
Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)


Biomaterial scaffolds have great potential to support efficient vascular differentiation of embryonic stem cells. Vascular cell fate-specific biochemical and biophysical cues have been identified and incorporated into three-dimensional (3D) biomaterials to efficiently direct embryonic vasculogenesis. The resulting vascular-like tissue can be used for regenerative medicine applications, further elucidation of biophysical and biochemical cues governing vasculogenesis, and drug discovery. In this chapter, we give an overview on the following: (1) developmental cues for directed differentiation of human embryonic stem cells (hESCs) into vascular cells, (2) 3D vascular differentiation in embryoid bodies (EBs), (3) preparation of 3D scaffolds for the vascular differentiation of hESCs, and (4) the most significant studies combining scaffolding and hESCs for development of vascular-like tissue.


Hyaluronic Acid Spinner Flask Vascular Differentiation PLGA Microparticle Biomaterial Scaffold 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported in part by NIH (grant HL060435). TPK was supported by the Swiss National Science Foundation (grant number PBELP3-127902). SA was supported by the FCT (grant number SFRH/BD/42871/2008). LF was supported by a Marie Curie-Reintegration Grant, MIT-Portugal program, Crioestaminal, Associação Viver a Ciência, and FCT (PTDC/SA-BEB/098468/2008; PTDC/CTM/099659/2008).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Thomas P. Kraehenbuehl
  • Sezin Aday
  • Lino S. Ferreira
    • 1
    • 2
  1. 1.Biocant, Centro de Inovação em BiotecnologiaCantanhedePortugal
  2. 2.Center of Neurosciences and Cell BiologyUniversity of CoimbraCoimbraPortugal

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