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Effect of Oxygenation on Stem-Cell Therapy for Myocardial Infarction

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Book cover Oxygen Transport to Tissue XXXII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 701))

Abstract

Stem-cell transplantation to treat acute myocardial infarction (MI) is gaining importance as a minimally invasive and potent therapy to replace akinetic scar tissue by viable myocardium. Our recent studies have shown that stem-cell transplantation marginally improvesmyocardial oxygenation in the infarct tissue leading to improvement in cardiac function. The aim of the present study was to determine the effect of hyperbaric oxygen (HBO) treatment on myocardial oxygenation and recovery of function in MI hearts. Fisher-344 rats were subjected to MI by permanently ligating the left-anterior-descending (LAD) coronary artery. The rats were then exposed to 100% O2 at a pressure of 2 atmospheres for 90 minutes, and the exposure was repeated for 5 days a week for 2 weeks. Adult bone-marrow-derived rat mesenchymal stem cells (MSC, 5x105 cells) were mixed with OxySpin (LiNc- BuO, oxygen sensor) and implanted in the infarct and peri-infarct regions of the heart. M-mode ultrasound echocardiography was performed at baseline and at 2 weeks post-transplantation. The myocardial pO2 in theMSC+HBOgroup (16.2±2.2 mmHg) was significantly higher when compared to untreated MI (3.8±1.9 mmHg) or MSC (9.8±2.3 mmHg) groups. In addition, there was a significant improvement in cardiac function, increased vessel density, and VEGF expression in MSC+HBO group compared to MSC group (p < 0.05). In conclusion, the results suggested a beneficial effect of HBO administration on stem-cell therapy for MI.

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References

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Authors and Affiliations

  1. Davis Heart and Lung Research Institute, Department of Internal Medicine, The Ohio State University, Columbus, OH, 43210, USA

    Mahmood Khan, Sarath Meduru, Ramasamy P. Pandian, Brian K. Rivera & Periannan Kuppusamy

Authors
  1. Mahmood Khan
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  2. Sarath Meduru
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  3. Ramasamy P. Pandian
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  4. Brian K. Rivera
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  5. Periannan Kuppusamy
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Corresponding author

Correspondence to Mahmood Khan .

Editor information

Editors and Affiliations

  1. , Department of Physiology & Biophysics, Case Western Reserve University School o, 10900 Euclid Avenue, Cleveland, 44106, Ohio, USA

    Joseph C. LaManna

  2. , Department of Nutrition, Case Western Reserve University, Cleveland, 44106, Ohio, USA

    Michelle A. Puchowicz

  3. , Department of Neurology, Case Western Reserve University, Cleveland, 44106, Ohio, USA

    Kui Xu

  4. , Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, NE1 7RU, United Kingdom

    David K Harrison

  5. Synthesizer, Inc., Westminster Rd 2773, Ellicott City, 21043, Maryland, USA

    Duane F. Bruley

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Khan, M., Meduru, S., Pandian, R.P., Rivera, B.K., Kuppusamy, P. (2011). Effect of Oxygenation on Stem-Cell Therapy for Myocardial Infarction. In: LaManna, J., Puchowicz, M., Xu, K., Harrison, D., Bruley, D. (eds) Oxygen Transport to Tissue XXXII. Advances in Experimental Medicine and Biology, vol 701. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7756-4_24

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