Abstract
Previously we reported that three imaging methods, dynamic contrast enhanced magnetic resonance imaging (DCE-MRI), T1ρ-MRI, and ultralow temperature NADH/flavoprotein fluorescence imaging (redox scanning), could differentiate the less metastatic human melanoma cell line A375P from a more metastatic line C8161 growing as mouse xenografts in nude mice (Li LZ et al. Adv. Exp. Med. Biol., 2007, 599:67-78; PNAS, 2009, 106:6608-6613). The more metastatic C8161 tumor was characterized by less blood perfusion/permeability, a more oxidized mitochondrial redox state in the tumor core, and a smaller T1ρ relaxation time constant averaged across the entire tumor section. In the current study, we have further probed the bioenergetic status and tissue microenvironment of these tumors by applying whole tumor phosphorous magnetic resonance spectroscopy (31P-MRS) to these two xenografts in a vertical bore 9.4-T Varian magnet. The phosphomonoester (PME)/βNTP ratio and intracellular pH value (pHi) were determined. The phosphomonoester (PME)/βNTP was higher in the more metastatic C8161 tumors (n=4) than in the less metastatic A375P tumors (n=4) (p < 0.1). No significant difference between the pHi of C8161 and A375P was observed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Li, L.Z., et al Predicting melanoma metastatic potential by optical and magnetic resonance imaging. Adv Exp Med Biol, 2007. 599: p. 67-78.
Li, L.Z., et al Quantitative magnetic resonance and optical imaging biomarkers of melanoma metastatic potential. Proceedings of the National Academy of Sciences of the United States of America, 2009. 106(16): p. 6608-6613.
Rofstad, E.K. Microenvironment-induced cancer metastasis. Int J Radiat Biol, 2000. 76(5): p. 589-605.
Subarsky, P. and R.P. Hill The hypoxic tumour microenvironment and metastatic progression. Clin Exp Metastasis, 2003. 20(3): p. 237-250.
Gillies, R.J., et al MRI of the tumor microenvironment. J Magn Reson Imaging, 2002. 16(4): p. 430-450.
Vaupel, P. and A. Mayer Hypoxia in cancer: significance and impact on clinical outcome. Cancer and Metastasis Reviews, 2007. 26(2): p. 225-239.
Dewhirst, M.W., et al Relation between pO(2), P-31 magnetic resonance spectroscopy parameters and treatment outcome in patients with high-grade soft tissue sarcomas treated with thermoradiotherapy. International Journal of Radiation Oncology Biology Physics, 2005. 61(2): p. 480-491.
Bhujwalla, Z.M., et al Estimations of intra- and extracellular volume and pH by P-31 magnetic resonance spectroscopy: effect of therapy on RIF-1 tumours. British Journal of Cancer, 1998. 78(5): p. 606-611.
Zhou, R., et al Intracellular acidification of human melanoma xenografts by the respiratory inhibitor m-iodobenzylguanidine plus hyperglycemia: A P-31 magnetic resonance spectroscopy study. Cancer Research, 2000. 60(13): p. 3532-3536.
Shukla-Dave, A., et al Prediction of treatment response of head and neck cancers with P-31 MR spectroscopy from pretreatment relative phosphomonoester levels. Academic Radiology, 2002. 9(6): p. 688-694.
Arias-Mendoza, F., M.R. Smith, and T.R. Brown, Predicting treatment response in non- Hodgkin’s lymphoma from the pretreatment tumor content of phosphoethanolamine plus phosphocholine. Academic Radiology, 2004. 11(4): p. 368-376.
Gatenby, R.A. and R.J. Gillies Why do cancers have high aerobic glycolysis? Nature Reviews Cancer, 2004. 4(11): p. 891-899.
Gatenby, R.A., et al Acid-mediated tumor invasion: a multidisciplinary study. Cancer Research, 2006. 66(10): p. 5216-5223.
Gillies, R.J., et al PH imaging. IEEE Engineering in Medicine and Biology Magazine, 2004. 23(5): p. 57-64
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this paper
Cite this paper
Li, L.Z., Zhou, R., Leeper, D.B., Glickson, J.D. (2011). 31P-MRS Studies of Melanoma Xenografts with Different Metastatic Potential. In: LaManna, J., Puchowicz, M., Xu, K., Harrison, D., Bruley, D. (eds) Oxygen Transport to Tissue XXXII. Advances in Experimental Medicine and Biology, vol 701. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7756-4_10
Download citation
DOI: https://doi.org/10.1007/978-1-4419-7756-4_10
Published:
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-7755-7
Online ISBN: 978-1-4419-7756-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)