Abstract
Peppers are one of the oldest vegetables known and are popular in worldwide cuisine. Members of the Capsicum genus could be considered accomplished chemists, as these plants synthesize a variety of phytochemicals with valued roles in human health and nutrition as well as sensory perception. For thousands of years, humans have selected peppers for traits that affect heat, color, and flavor in the fruit. Heat in pepper fruit is attributed to production of capsaicinoids in the placenta; this alkaloid binds to nociceptive pain receptors in mammals. Color in pepper fruit is due to a combination of pigments: chlorophylls, carotenoids, and anthocyanins accumulating in the fruit wall or pericarp of the fruit resulting in green, yellow, or purple fruit at physiological immature stages and yellow, red, or orange fruit at mature stages. Flavor is the least characterized chemistry, but is in part the result of monoterpenoids and aliphatic aldehydes, which also accumulate in the fruit wall. The synthesis and accumulation of all these phytochemicals are dependent on the genetic background of the pepper, the developmental stage of the fruit, and environmental factors.
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References
Perry L, Dickau R, Zarillo S, Holst I, Pearsall DM, Piperno DR, Berman MJ, Cooke RG, Rademaker K, Ranere AJ, Ramond JS, Sandweiss DJ, Scaramelli F, Tarble K, Zeidler JA (2007) Starch fossils and the domestication and dispersal of chili peppers (Capsicum spp. L.) in the Americas. Science 315:986–988
FAO (2006) Food and Agriculture Organization of the United States. http://www.fao.org
USDA (2008) Vegetables and Melons Outlook. http://www.ers.usda.gov/publications/vgs/2008/08Aug/VGS328.pdf
Pickersgill B (2007) Domestication of plants in the Americas: insights from Mendelian and molecular genetics. Ann Bot 100:925–940
Bosland PW (1996) Capsicums: Innovative uses of an ancient crop. In: Janick J (ed) Progress in new crops. ASHS Press, Arlington, VA, 479–487
Livingstone KD, Lackney VK, Blauth JR, Van Wijk R, Jahn MM (1999) Genome mapping in capsicum and the evolution of genome structure in the Solanaceae. Genetics 152:1183–1202
Wu F, Eannetta NT, Xu Y, Durrett R, Mazourek M, Jahn MM, Tanksley SD (2009) A COSII genetic map of the pepper genome provides a detailed picture of synteny with tomato and new insights into recent chromosome evolution in the genus Capsicum. Theor Appl Genet 118:1279–1293
Paran I, Van Der Knaap E (2007) Genetic and molecular regulation of fruit and plant domestication traits in tomato and pepper. J Exp Bot 58:3841–3852
Wang D, Bosland P (2006) The genes of Capsicum. HortScience 41:1169–1187
DellaPenna D, Pogson BJ (2006) Vitamin synthesis in plants: tocopherols and carotenoids. Annu Rev Plant Biol 57:711–738
Deepa N, Kaur C, George B, Singh B, Kapoor HC (2005) Antioxidant constituents in some sweet pepper (Capsicum annuum L.) genotypes during maturity. Food Sci Technol 40:121–129
Gross J (1991) Pigments in vegetables: chlorophylls and carotenoids. Van Nostand Reinhold, New York, NY
Sergio AR, Paiva MD, Rusell RM (1999) β-carotene and other carotenoids as antioxidants. J Amer Coll Nutr 18:426–433
Schemske DW, Bradshaw HD (1999) Pollinator preference and the evolution of floral traits in monkeyflowers (Mimulus). Proc Natl Acad Sci USA 96:11910–11915
Wang HC, Huang XM, Hu GB, Yang Z, Huang HB (2005) A comparative study of chlorophyll loss and its related mechanism during fruit maturation in the pericarp of fast- and slow-degreening litchi pericarp. Sci Hortic 106:247–257
Smith PG (1950) Inheritance of brown and green mature color in peppers. J Hered 41:138–140
Lightbourn GJ, Griesbach RJ, Novotny JA, Clevidence BA, Rao DD, Stommel JR (2008) Effects of anthocyanin and carotenoid combinations on foliage and immature fruit color of Capsicum annuum L. J Hered 99:105–111
Deli J, Molnar P, Toth G (2001) Carotenoid composition in the fruits of red paprika (Capsicum annuum var. lycopersiciforme rubrum) during ripening; biosynthesis of carotenoids in red paprika. J Agric Food Chem 49:1517–1523
Matsufuji H, Nakamura H, Chino M, Takeda M (1998) Antioxidant activity of capsanthin and the fatty acid esters in paprika (Capsicum annuum). J Agric Food Chem 46:3468–3472
Wall MM, Waddell CA, Bosland PW (2001) Variation in β-carotene and total carotenoid content in fruits of Capsicum. HortScience 36:746–749
Auldrige ME, McCarty DR, Klee HJ (2006) Plant carotenoid cleavage oxygenases and their apocarotenoid products. Curr Opin Plant Biol 9:315–321
von Lintig J, Vogt K (2004) Vitamin A formation in animals: molecular identification and functional characterization of carotenoid cleaving enzymes. J Nutr 134:251S-256S
USDA (2000) Nutrition and Your Health: Dietary Guidelines for Americans. http://www.cnpp.usda.gov/Publications/DietaryGuidelines/2000/2000DGProfessionalBooklet.pdf.
Purseglove J, Brown E, Green C, Robbins S (1981) Spices. Longman, London
Hugueney P, Badillo A, Chen H-C, Klein A, Hirschberg J, Camara B, Kuntz M (1995) Metabolism of cyclic carotenoids: a model for the alteration of this biosynthetic pathway in Capsicum annuum chromoplasts. Plant J 8:417–424
Fraser P, Bramley P (2004) The biosynthesis and nutritional uses of carotenoids. Prog Lipid Res 43:228–265
Hugueney P, Bouvier F, Badillo A, Quennemet J, d'Harlingue A, Camara B (1996) Developmental and stress regulation of gene expression for plastid and cytosolic isoprenoid pathways in pepper fruits. Plant Physiol 111:619–626
Cunningham FX, Grantt E (1998) Genes and enzymes of carotenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol 49:557–583
Romer S, Hugueney P, Bouvier F, Camara B, Kuntz M (1993) Expression of the genes encoding the early carotenoid biosynthetic enzymes in Capsicum annuum. Biochem Biophys Res Commun 196:1414–1421
Bouvier F, Keller Y, d'Harlingue A, Camara B (1998) Xanthophyll biosynthesis: molecular and functional characterization of carotenoid hydroxlases from pepper fruits (Capscium annuum L.). Biochim Biophys Acta 1391:320–328
Bouvier F, d'Harlingue A, Hugueney P, Marin E, Marion-Poll A, Camara B (1996) Xanthophyll biosynthesis: cloning, expression, functional reconstitution, and regulation of β-cyclohexenyl carotenoid epoxidase from pepper (Capsicum annuum). J Biol Chem 271:28861–22867
Bouvier F, Hugueney P, d'Harlingue A, Kuntz M, Camara B (1994) Xanthophyll biosynthesis in chromoplasts: isolation and molecular cloning of an enzyme catalyzing the conversion of 5,6-epoxycarotenoid into ketocarotenoid. Plant J 6:45–54
Hurtado-Hernandez H, Smith PG (1985) Inheritance of mature fruit color in Capsicum annuum L. J Hered 76:211–213
Lefebvre V, Kuntz M, Camara B, Palloix A (1998) The capsanthin-capsorubin synthase gene: a candidate gene for the y locus controlling the red fruit colour in pepper. Plant Mol Biol 36:785–789
Huh JH, Kan BC, Nahm SH, Kim S, Ha KS, Lee MH, Kim BD (2001) A candidate gene approach identified phytoene synthase as the locus for mature fruit color in red pepper (Capsicum spp.). Theor Appl Genet 102:524–530
Guzman I, Hamby S, Romero J, Bosland PW, O’Connell MA (2010) Variability of Carotenoid Biosynthesis in Orange Colored Capsicum spp. Plant Science, 179:49–59
Lang YQ, Yanagawa S, Sasanuma T, Sasakuma T (2004) Orange fruit color in Capsicum due to deletion of capsanthin-capsorubin synthesis gene. Breed Sci 54:33–39
Popovsky S, Paran I (2000) Molecular genetics of the y locus in pepper: its relation to capsanthin-capsorubin synthase and to fruit color. Theor Appl Genet 101:86–89
Thorup TA, Tanyolac B, Livingstone KD, Popovsky S, Paran I, Jahn M (2000) Candidate gene analysis of organ pigmentation loci in the Solanaceae. Proc Natl Acad Sci USA 97:11192–11197
Hornero-Mendez D, Minguez-Mosquera MI (2000) Xanthophyll esterification accompanying carotenoid overaccumulation in chromoplasts of Capsicum annuum ripening fruits is a constitutive process and useful for ripeness index. J Agric Food Chem 48:1617–1622
Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering provitamin A (β-carotene) biosynthesis pathway into (carotenoid-free) rice endosperm. Science 287:303–305
Paran I, Van Der Voort JR, Lefebvre V, Jahn MM, Landry L, Van Schriek M, Tanyolac B, Caranta C, Chaim AB, Livingstone K, Palloix A, Peleman J (2004) An integrated genetic linkage map of pepper (Capsicum spp.). Mol Breed 13:251–261
Daskalov S, Poulos JM (1994) Updated Capsicum gene list. Capsicum Eggplant Newsl 13:15–26
Ben Chaim A, Paran I, Grube R, Jahn MM, Van Wijk R, Peleman J (2001) QTL mapping of fruit related traits in pepper (Capsicum annuum). Theor Appl Genet 102:1016–1028
Borovsky Y, Oren-Shamir M, Ovadia R, De Jong W, Paran I (2004) The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia. Theor Appl Genet 109:23–29
Billing J, Sherman PW (1998) Antimicrobial functions of spices: why some like it hot. Quart Rev Biol 73:3–49
Govindarajan V, Sathyanarayana M (1991) Capsicum - production, technology, chemistry, and quality. Part V - Impact on physiology, pharmacology, nutrition and metabolism; structure, pungency, pain and desensitization sequences. Crit Rev Food Sci Nutr 29:435–474
Winter J, Bevan S, Campbell EA (1995) Capsaicin and pain mechanisms. Brit J Anaesth 75:157–168
Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D (1997) The capsaicin receptor: a heat-activated ion channel in pain pathway. Nature 389:816–824
Tominaga M, Caterina MJ, Malmberg AB, Rosen TA, Gilbert H, Skinner K, Raumann BE, Basbaum AI, Julius D (1998) The cloned capsaicin receptor integrates multiple pain-producing stimuli. Neuron 21:531–543
Bennett DJ, Kirby GW (1968) Constitution and biosynthesis of capsaicin. J Chem Soc C:442–446
Leete E, Louden M (1968) Biosynthesis of capsaicin and dihydrocapsaicin in Capsicum frutescens. J Am Chem Soc 90:6837–6841
Krajewska A, Powers J (1988) Sensory properties of naturally occurring capsaicinoids. J Food Sci 53:902–905
Collins MD, Mayer-Wasmund L, Bosland PW (1995) Improved method for quantifying capsaicinoids in Capsicum using high performance liquid chromatography. HortScience 30:137–139
Tewksbury JJ, Nabhan GP (2001) Directed deterrence by capsaicin in chillies. Nature 412:403–404
Tewksbury J, Reagan K, Machnicki N, Carlo T, Haak D, Calderon-Penaloza A, Levey D (2008) Evolutionary ecology of pungency in wild chilies. Proc Natl Acad Sci USA 105:11808–11811
Fujiwake H, Suzuki T, Iwai K (1982) Capsaicinoid formation in the protoplast from the placenta of Capsicum fruits. Agric Biol Chem 46:2591–2592
Fujiwake H, Suzuki T, Iwai K (1982) Intracellular distributions of enzymes and intermediates involved in biosynthesis of capsaicin and its analogues in Capsicum fruits. Agric Biol Chem 46:2685–2689
Hall R, Holden M, Yeoman M (1987) The accumulation of phenylpropanoid and capsaicinoid compounds in cell cultures and whole fruit of the chili pepper, Capsicum frutescens Mill. Plant Cell Tissue Organ Cult 8:163–176
Iwai K, Suzuki T, Fujiwake H (1979) Formation and accumulation of pungent principle of hot pepper fruits, capsaicin and its analogues in Capsicum annuum var. annuum cv. Karayatsubusa at different growth stages after flowering. Agric Biol Chem 43:2493–2498
Ochoa-Alejo N, Gomez-Peralta JE (1993) Activity of enzymes involved in capsaicin biosynthesis in callus tissue and fruits of chili pepper (Capsicum annuum L.). J Plant Physiol 141:147–152
Sukrasno N, Yeoman MM (1993) Phenylpropanoid metabolism during growth and development of Capsicum frutescens fruits. Phytochemistry 32:839–844
Suzuki T, Kawada T, Iwai K (1981) The precursors affecting the composition of capsaicin and its analogues in the fruits of Capsicum annuum var. annuum cv. Karayatsubusa. Agric Biol Chem 45:535–537
Aluru MR, Mazourek M, Landry LG, Curry J, Jahn MM, O'Connell MA (2003) Differential expression of fatty acid synthase genes, Acl, Fat and Kas in Capsicum fruit. J Exp Bot 54:1655–1664
Curry J, Aluru M, Mendoza M, Nevarez J, Melendrez M, O'Connell MA (1999) Transcripts for capsaicinoid biosynthetic genes are differentially accumulated in pungent and non-pungent Capsicum spp. Plant Sci 148:47–57
Kim M, Kim S, Kim S, Kim B-D (2001) Isolation of cDNA clones differentially accumulated in the placenta of pungent pepper by suppression subtractive hybridization. Mol Cells 11:213–219
Mazourek M, Pujar A, Borovsky Y, Paran I, Mueller L, Jahn MM (2009) A dynamic interface for capsaicinoid systems biology. Plant Physiol 150:1806–1821
Orlova I, Marshall-Colon A, Schnepp J, Wood B, Varbanova M, Fridman E, Blakeslee JJ, Peer WA, Murphy AS, Rhodes D, Pichersky E, Dudareva N (2006) Reduction of benzoid synthesis in petunia flowers reveals multiple pathways to benzoic acid and enhancement in auxin transport. Plant Cell 18:3458–3475
Thiele R, Mueller-Seitz E, Petz M (2008) Chili pepper fruits: presumed precursors of fatty acids characteristic for capsaicinoids. J Agric Food Chem 56:4219–4224
Fujiwake H, Suzuki T, Oka S, Iwai K (1980) Enzymatic formation of capsaicinoid from vanillylamine and iso-type fatty acids by cell-free extracts of Capsicum annuum var. annuum cv. Karayatsubusa. Agric Biol Chem 44:2907–2912
Stewart C, Kang B-C, Liu K, Mazourek M, Moore SL, Yoo EY, Kim B-D, Paran I, Jahn MM (2005) The Pun1 gene for pungency in pepper encodes a putative acyltransferase. Plant J 42:675–688
Stewart C, Mazourek M, Stellari GM, O’Connell MA, Jahn M (2007) Genetic control of pungency in C. chinense via the Pun 1 locus. J Exp Bot 58:979–991
Suzuki T, Fujiwake H, Iwai K (1980) Intracellular localization of capsaicin and its analogues, capsaicinoid, in Capsicum fruit. 1. Microscopic investigation of the structure of the placenta of Capsicum annuum var. annuum cv. Karayatsubusa. Plant Cell Physiol 21:839–853
Ohta Y (1963) Physiological and genetical studies on the pungency of Capsicum IV. Secretory organ, receptacles and distribution of capsaicin in the Capsicum fruits. Jap J Breed 12:179–183
Heiser CB, Smith PG (1953) The cultivated Capsicum peppers. Econ Bot 7:214–227
Blum E, Liu K, Mazourek M, Yoo EY, Jahn MM, Paran I (2002) Molecular mapping of the C locus for presence of pungency in Capsicum. Genome 45:702–705
Blum E, Mazourek M, O’Connell MA, Curry J, Thorup T, Liu K, Jahn MM, Paran I (2003) Molecular mapping of capsaiciniod biosynthesis genes and QTL analysis for capsaicinoid content in Capsicum. Theor Appl Genet 108:79–86
Eshbaugh WH (1980) The taxonomy of the genus Capsicum (Solanaceae). Phytologia 47:153–166
Tewksbury J, Manchego C, Haak D, Levey D (2006) Where did the chili get its spice? Biogeography of capsiaicinoid production in ancestral wild chili species. J Chem Ecol 32:547–564
Iwai K, Lee K-R, Kobashi M, Suzuki T (1977) Formation of pungent principles in fruits of sweet pepper, Capsicum annuum L. var. grossum during post-harvest ripening under continuous light. Agric Biol Chem 41:1873–1876
Iwai K, Suzuki T, Lee K-R, Kobashi M, Oka S (1977) In vivo and in vitro formation of dihydrocapsaicin in sweet pepper fruits, Capsicum annuum L. var. grossum. Agric Biol Chem 41:1877–1882
Votava E, Bosland P (2002) Novel sources of non-pungency in Capsicum species. Capsicum Eggplant Newslett 21:66–68
Garces-Claver A, Gil-Ortega R, Alvarez-Fernandez A, Arnedo-Andres M (2007) Inheritance of capsaicin and dihydrocapsaicin, determined by HPLC-ESI/MS, in an intraspecific cross of Capsicum annuum L. J Agric Food Chem 55:6951–6957
Zewdie Y, Bosland PW (2000) Capsaicinoid inheritance in an interspecific hybridization of Capsicum annuum x C. chinense. HortScience 125:448–453
Ben Chaim A, Borovsky Y, Falise M, Mazourek M, Kung BC, Paran I, Jahn MM (2006) QTL analysis for capsaicinoid content in Capsicum. Theor Appl Genet 113:1481–1490
Harvell K, Bosland PW (1997) The environment produces a significant effect on pungency of chiles. HortScience 32:1292
Zewdie Y, Bosland PW (2000) Evaluation of genotype, environment, and genotype-by-environment interaction for capsaicinoids in Capsicum annuum L. Euphytica 111:185–190
Mueller-Seitz E, Hiepler C, Petz M (2008) Chili pepper fruits: content and pattern of capsaicinoids in single fruits of different ages. J Agric Food Chem 56:12114–12121
Zewdie Y, Bosland PW (2000) Pungency of chile (Capsicum annuum L.) fruit is affected by node position. HortScience 35:1174
Buttery RG, Seifert RM, Guadagni DG, Ling LC (1969) Characterization of some volatile constituents of bell peppers. J Agric Food Chem 17:1322–1327
Zimmermann M, Schieberle P (2000) Important odorants of sweet bell pepper powder (Capsicum annuum cv. annuum): differences between samples of Hungarian and Moroccan origin. Eur Food Res Technol 211:175–180
Mazidaa MM, Sallehb MM, Osman H (2005) Analysis of volatile aroma compounds of fresh chilli (Capsicum annuum) during stages of maturity using solid phase microextraction (SPME). J Food Comp Anal 18:427–437
Acknowledgment
This work was supported in part by NM Agricultural Experiment Station, NIH grants GM08136 and GM61222, and USDA grant CSREES 2009-34604-19939.
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Guzman, I., Bosland, P.W., O’Connell, M.A. (2011). Heat, Color, and Flavor Compounds in Capsicum Fruit. In: Gang, D. (eds) The Biological Activity of Phytochemicals. Recent Advances in Phytochemistry, vol 41. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7299-6_8
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