Abstract
The simplest device to measure interaction of a sample with light is the absorbance spectrophotometer. Monochromatic light is passed through the sample (or, in some cases, reflected from it). If absorption occurs, the light intensity that arrives at a detector is lower than what would arrive in the absence of the sample.
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© 2011 Springer Science+Business Media, LLC
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Buxbaum, E. (2011). Photometry. In: Biophysical Chemistry of Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7251-4_5
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DOI: https://doi.org/10.1007/978-1-4419-7251-4_5
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Publisher Name: Springer, Boston, MA
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