Abstract
There are two principal routes to radio-labelled proteins: metabolic labelling in vivo with labelled amino acids (in particular 35S labelled Cys and/or Met) and chemical introduction in vitro. Metabolic labelling has the advantage that it will not interfere with the proteins function, however, only a small part of the radioactivity used ends up in the protein you are interested in. Some stays in small molecules, some is bound to other proteins. Chemical labelling is performed on an already purified protein, so only unreacted label must be removed by gel filtration.
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Buxbaum, E. (2011). Detection Methods. In: Biophysical Chemistry of Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7251-4_23
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DOI: https://doi.org/10.1007/978-1-4419-7251-4_23
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