Self-Feedback in Actin Polymerization

  • Anders E. CarlssonEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 736)


Polymerization of actin, which is crucial for functions such as cell migration, membrane ruffling, cytokinesis, and endocytosis, must be tightly regulated in order to preserve an adequate supply of free actin monomers to respond to changing external conditions. The paper will describe mechanisms by which F-actin feeds back on its own assembly, thus regulating itself. I will present the experimental evidence for such feedback terms, discuss their use in current models of actin dynamics in cells, and present preliminary calculations for the role of feedback in transient endocytic actin patches. These calculations suggest a partial homeostasis of F-actin, in which the F-actin peak height depends only weakly on the actin filament nucleation rate.


Actin Filament Actin Polymerization Fluorescence Recovery After Photobleaching Actin Patch Inhibit Actin Polymerization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the National Institutes of Health under Grant R01 GM086882.


  1. 1.
    Pollard TD, Borisy GG (2003) Cellular motility driven by assembly and disassembly of actin filaments. Cell 112:453–456PubMedCrossRefGoogle Scholar
  2. 2.
    Alon, U (2007) An introduction to systems biology. Taylor and Francis, New YorkGoogle Scholar
  3. 3.
    Pantaloni D, Boujemaa R, Didry D, Gounon P, Carlier MF (2000) The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteins. Nat Cell Biol 2:385–391PubMedCrossRefGoogle Scholar
  4. 4.
    Amann KJ, Pollard TD (2001) The Arp2/3 complex nucleates actin filament branches from the sides of existing filaments. Nat Cell Biol 3:306–310PubMedCrossRefGoogle Scholar
  5. 5.
    Carlsson AE, Wear MA, Cooper JA (2004) End vs. side branching by Arp2/3 complex. Biophys J 86:1074–1081PubMedCrossRefGoogle Scholar
  6. 6.
    Goley ED, Ohkawa T, Mancuso J, Woodruff JB, D’Alessio JA, Cande WZ, Volkman LE, Welch MD (2006) Dynamic nuclear actin assembly by Arp2/3 complex and a baculovirus wasp-like protein. Science 314:464–467PubMedCrossRefGoogle Scholar
  7. 7.
    Tehrani S, Tomasevic N, Weed S, Sakowicz R, Cooper J (2007) Src phosphorylation of cortactin enhances actin assembly. Proc Natl Acad Sci 104:8827–88323CrossRefGoogle Scholar
  8. 8.
    Weiner OD, Marganski WA, Wu LF, Altschuler SJ, Kirschner MW (2007) An actin-based wave generator organizes cell motility. PLoS Biol 5:2053–2063CrossRefGoogle Scholar
  9. 9.
    Kaksonen M, Toret CP, Drubin DG (2005) A modular design for the clarhtin- and actin-mediated endocytosis machinery. Cell 123(2):305–320PubMedCrossRefGoogle Scholar
  10. 10.
    Woodring PM, Hunter T, Wang JYJ (2001) Inhibition of c-abl tyrosine kinase activity by filamentous actin. J Biol Chem 276:27104–27110PubMedCrossRefGoogle Scholar
  11. 11.
    Lanier LM, Gertler FB (2000) From Abl to actin: Abl tyrosine kinase and associated proteins in growth cone motility. Curr Opin Neurobiol 10:80–87PubMedCrossRefGoogle Scholar
  12. 12.
    Ganguly A, Saxena R, Chattopadhyay A (2011) Reorganization of the actin cytoskeleton upon G-protein coupled receptor signaling. Biochim Biophys Acta-Biomembranes 1808:1921–1929CrossRefGoogle Scholar
  13. 13.
    Ganguly S, Pucadyil A, Chattopadhyay A (2008) Actin cytoskeleton-dependent dynamics of human serotonin1a receptor correlates with receptor signaling. Biophys J 95:451–463PubMedCrossRefGoogle Scholar
  14. 14.
    Carlsson AE (2010) Actin dynamics: from nanoscale to microscale. Ann Rev Biophys 39:91–110CrossRefGoogle Scholar
  15. 15.
    Doubrovinski K, Kruse K (2008) Cytoskeletal waves in the absence of molecular motors. Europhys Lett 83:18003CrossRefGoogle Scholar
  16. 16.
    Whitelam S, Bretschneider T, Burroughs NJ (2009) Transformation from spots to waves in a model of actin pattern formation. Phys Rev Lett 102:198103PubMedCrossRefGoogle Scholar
  17. 17.
    Carlsson AE (2010) Dendritic actin filament nucleation causes traveling waves and patches. Phys Rev Lett 104:228102PubMedCrossRefGoogle Scholar
  18. 18.
    Liu J, Sun Y, Drubin DG, Oster GF (2009) The mechanochemistry of endocytosis. PLoS Biol 7:e1000204CrossRefGoogle Scholar
  19. 19.
    Padrick SB, Cheng HC, Ismail AM, Panchal SC, Doolittle LK, Kim S, Skehan BM, Umetani J, Brautigam CA, Leong JM, Rosen MK (2008) Hierarchical regulation of WASP/WAVE proteins. Mol Cell 32:426–438PubMedCrossRefGoogle Scholar
  20. 20.
    Kelton KF, Greer AL (2007) Nucleation in condensed matter: applications in materials and biology. Elsevier, BostonGoogle Scholar
  21. 21.
    Galletta BJ, Chuang DY, Cooper JA Distinct roles for Arp2/3 regulators in actin assembly and endocytosis. PLoS Biol 6:72–85Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of PhysicsWashington UniversitySt. LouisUSA

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