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Genome-Wide Expression Profiles of Amygdala and Hippocampus in Mice After Fear Conditioning

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Genomics, Proteomics, and the Nervous System

Part of the book series: Advances in Neurobiology ((NEUROBIOL,volume 2))

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Abstract

The amygdala and hippocampus are known to be involved in the formation of fear conditioning memories, and both contextual and cued fear memory requires activation of the NMDA receptors. However, the global molecular responses to fear conditioning in the amygdala and hippocampus are still poorly understood. We have systematically analyzed the gene expression profiles in these two brain regions of mice after fear conditioning treatment using high-density microarrays containing 11,000 genes and expressed sequence tags. A total of 222 genes in the amygdala and 145 genes in the hippocampus exhibit dynamic changes in their expression levels. The overall patterns of gene expression as well as the individual genes are drastically different in amygdala and hippocampus. However, a number of genes display similar regulatory responses in both brain regions. Based on the expression kinetics, the genes can be further grouped into eight and six unique clusters in amygdale and hippocampus, respectively. Our gene expression analysis demonstrates that different genomic responses are initiated in the amygdala and hippocampus, two brain regions that play distinct roles in associative memory formation.

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Abbreviations

AMPA:

Amino-3-hydroxy-5-methyl-4-isoxalone propionic acid

APP:

Amyloid precursor protein

ARF:

ADP-ribosylation factor

CaMKII:

Calcium-calmodulin-kinase II

CCT:

Chaperonin-containing TCP

CS:

Conditioned stimulus

Cx30:

Connexin-30

ESTs:

Expressed sequence tags

FXR1:

Fragile-X-related gene 1

GABA:

Gamma-aminobutyric acid

GABARAP:

GABA receptor-associated protein

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GluR1:

Glutamate receptor 1

IAP:

Integrin-associated protein

KLC1:

Kinesin light chain 1

MAP:

Microtubule-associated protein

MOBP:

Myelin-associated oligodendrocytic basic protein

NF1:

Neurofibromatosis type 1

NMDA:

N-methyl-d-aspartate

NP25:

Neuronal protein 25

NSG1:

Neuron-specific gene family member 1

OSP:

Oligodendrocyte-specific protein

PIP5K:

Phosphatidylinositol-4-phosphate-5-kinase

PKC:

Protein kinase C

PLD:

Phospholipase D

PLP:

Proteolipid protein

PP:

Protein phosphatases

PP1:

Protein phosphatases 1

PP2A:

Protein phosphatases 2A

Stat3:

Signal transducer and activator of transcription

US:

Unconditioned stimulus

VAMP:

Vesicle-associated membrane protein

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Acknowledgements

This work was supported in part by grants from the Shanghai Municipal Education Commission and the Ministry of Science and Technology of China (2003AA221061), and grants from the Science and Technology Commission of Shanghai Municipality (05PJ14044 and 06DZ19002).

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  1. Key Laboratory of Brain Functional Genomics, MOE & STCSM, Shanghai Institute of Brain Functional Genomics, East China Normal University, 3663 Zhongshan Road (N), Shanghai, 200062, China

    Yinghe Hu

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Correspondence to Yinghe Hu .

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  1. Nathan S. Kline Institute for, Psychiatric Research, New York University, Old Orangeburg Road 140, Orangeburg, 10962, New York, USA

    James D Clelland

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Zhao, Z., Hu, Y. (2011). Genome-Wide Expression Profiles of Amygdala and Hippocampus in Mice After Fear Conditioning. In: Clelland, J. (eds) Genomics, Proteomics, and the Nervous System. Advances in Neurobiology, vol 2. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7197-5_12

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