Abstract
In vitro culture techniques represent an excellent tool for enabling our mind to understand those processes occurring along the life cycle of plants and also for realising successful benefits of their applications. This chapter summarises the in vitro culture of ferns from spore to sporophyte, giving details of how to proceed for achieving optimal performance of each phase involved in the life cycle, defining the cultural requirements leading to a greater production of these plants.
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References
Amaki, W., and Higuchi, H. 1991. A possible propagation system of Nephrolepis, Asplenium, Pteris, Adiantum,and Rhumora through tissue culture. Act. Hort. 300: 237–243.
Beck, M.J., and Caponetti, J.D. 1983. The effects of kinetin and naphtalenacetic acid in vitro shoot multiplication and rooting in the fishtail fern. Am. J. Bot. 70:1–7.
Bhambie, S., and Gupta, B. 1994. Induced apogamy and apospory in Adiantum capillus-veneris L. J. Ind. Bot. Soc.73:25–28.
Bordonneau, M., and Tourte, Y. 1987. Potentialites ontogenetique des tissues gametophytiques chez le Pilularia globulifera L. C. R. Acad. Aci. Paris Ser. III, 304:437–440.
Camloh, M., Gogala, N., and Rode, J. 1994. Plant regeneration from leaf explants of the fern Platycerium bifurcatum in vitro. Sci. Hortic. 56:257–266.
Camloh, M., Ravnikar, M., and Zel, J. 1996. Jasmonic acid promotes division of fern protoplasts, elongation of rhizoids and early development of gametophytes. Physiol. Plant. 97:659–664.
Chang, H.C., Agrawal, D.C., Kuo, C.L., Wen, J.L., Chen, C.C., and Tsay, H.S. 2007. In vitro culture of Drynaria fortunei, a fern species source of Chinese medicine “Gu-Sui-Bu”. In Vitro Cell. Dev. Biol. Plant 43:133–139.
Chou, H.M., Huang, Y.M., Wong, S.L., Hsieh, T.H., Hsu, S.Y., and Chiou, W.L. 2007. Observations of gametophytes and juvenile sporophytes of Archangiopteris somai Hayata (Marattiaceae), an endangered fern in Taiwan. Botanical Stud. 48:205–213.
Cox, J., Bhatia, P., and Ashwath, N. 2003. In vitro spore germination of the fern Schizaea dichotoma. Sci. Hort. 97:369–378.
Deberg, P. 1994. In vitro culture of ornamentals. In Plant Cell and Tissue Culture, ed. I.K. Vasil and T.A. Thorpe, pp. 561–573. Kluwer Academic Publishers, Dordrecht, the Netherlands.
DeSoto, L, Quintanilla, L.G., and Méndez M. 2008. Environmental sex determination in ferns: effects of nutrient availability and individual density in Woodwardia radicans. J. Ecol. 96:1319–1327.
Dyer, A.F. 1979. The culture of fern gametophytes for experimental investigation. In: The Experimental Biology of Ferns, ed. A.F. Dyer, pp.253–305. Academic Press, London.
Fay, M.F. 1994. In what situation is in vitro culture appropriate to plant conservation? Biodivers. Conserv. 3:176–183.
Fernández, H., Bertrand, A.M., and Sánchez-Tamés R. 1993. In vitro regeneration of Asplenium nidus L. from gametophytic and sporophytic tissue. Sci Hort. 56:71–77.
Fernández, H., Bertrand, A.M., and Sánchez-Tamés, R. 1996a. Influence of tissue culture conditions on apogamy in Dryopteris affinis ssp. affinis. Plant Cell Tissue Organ Cult. 45:93–97.
Fernández, H., Bertrand, A.M., and Sánchez-Tamés, R. 1996b. Micropropagation and phase change in Blechnum spicant and Pteris ensiformis. Plant Cell Tissue Organ Cult. 44:261–265.
Fernández, H., Bertrand, A.M., and Sánchez-Tamés, R. 1997a. Gemmation in Osmunda regalis L. gametophyte cultured in vitro. Plant Cell Rep. 16:358–362.
Fernández, H., Bertrand, A., and Sánchez-Tamés, R. 1997b. Plantlet regeneration in Asplenium nidus L. and Pteris ensiformis L. by homogenization of BA treated rhizomes. Sci. Hort. 68:243–247.
Fernández, H., Bertrand, A., and Sánchez-Tamés, R. 1997c. Gametophyte culture in vitro and antheridiogen activity in Blechnum spicant. Plant Cell Tissue Organ Cult. 50:71–74.
Fernández, H., Bertrand, A., and Sánchez-Tamés, R. 1999. Biological and nutritional aspects involved in fern multiplication. Plant Cell Tissue Organ Cult. 56:211–214.
Fernández, H., and Revilla, M.A. 2003. In vitro culture of ornamental ferns. Plant Cell Tissue Organ Cult. 73:1–13.
Fernández, H., Arbesú, R., Nistal, A.M., Menéndez, M., and Revilla, M.A. 2007. Preservation and characterization by confocal microscopy of fern spores. Adv. Hort. Sci. 21:203–206.
Hamilton, R.G., and Lloyd, R.M. 1991. Antheridiogen in the wild: The development of fern communities. Funct. Ecol. 5:804–809.
Harper, K. 1976. Asexual multiplication of Leptosporangiatae ferns through tissue culture. M.Sc. University of California.
Haufler, C.H., and Ranker, T.A. 1985. Differential antheridiogen response and evolutionary mechanisms in Cystopteris. Am. Bot. 72:659–665.
Hicks G., and von Aderkas P. 1986. A tissue culture of the ostrich fern Matteuccia struthiopteris L. Todaro. Plant Cell Tissue Organ Cult. 5:199–204.
Higuchi H., Amaki W., and Suzuki S. 1987. In vitro propagation of Nephrolepis cordifolia Prsel. Sci. Hort. 32:105–113.
Higuchi H., and Amaki W. 1989. Effects of 6-benzyladenine on the organogenesis of Asplenium nidus L. through in vitro propagation. Sci. Hort. 37:351–359.
Hirsch, A.M. 1975. The effect of sucrose on the differentiation of excised fern leaf tissue into either gametophytes or sporophytes. Plant Physiol. 56:390–393.
Hua, W., Ping-Ting, C., Li-Ping, Y., and Long-Qing, C. 2009. An efficient method for surface sterilization and sowing fern spores in vitro culture. Am. Fern J. 99:227–231.
Huang, Y.M, Chou, H.M., and Chiou, W.L. 2004. Density affects gametophyte growth and sexual expression of Osmunda cinnamomea (Osmundaceae: Pteridophyta). Ann. Bot. 94:229–232.
Klekowski, E.J. Jr 1969. Reproductive biology of the Pteridophyta. II. Theoretical considerations. Bot. J. Linn. Soc. 62:347–359.
Klekowski, E.J. Jr 1970. Reproductive biology of the Pteridophyta. IV. An experimental study of mating systems in Ceratopteris thalictroides (L.) Brong. Bot. J. Linn. Soc. 63:153–169.
Knop, W. 1865. Quantitative untersuchungen uber die ernahrungsprozesse der pflanzen. Landwirsch Vers Stn. 7:93–107.
Knudson, L. 1946. A nutrient solution for the germination of orchid seed. Bull. Am. Orchid Soc. 15:214–217.
Korpelainen, H. 1994. Growth, sex determination and reproduction of Dryopteris filix-mas (L.) Schott gametophytes under shifting nutritional conditions. Bot. J. Linn. Soc. 114:357–366.
Kshirsagar, M.R., and Mehta, A.R. 1978. In vitro studies in ferns: growth and differentiation in rhizome callus of Pteris vittata. Phytomorphology 28: 51–58.
Kuriyama, A., Kobayashi, T., Hayashi, S., and Maeda, M. 2004. Medium composition for the production of sporophytes of the fern Adiantum capillus-veneris. J. Jap. Soc. Hort. Sci. 73:580–582.
Kwa, S.H., Wee, Y.C., and Loh, C.S. 1988. Production of aposporous gametophytes from Drymoglossum piloselloides (L.) Presl. fronds strips. Plant Cell Rep. 7:142–143.
Kwa, S.H., Wee, Y.C., and Loh, C.S. 1990. Production of first- and second-generation aposporous gametophytes from Pyrrosia piloselloides (L.) Price frond strips cultured in vitro. Plant Cell Rep. 8:530–533.
Kwa, S.H., Wee, Y.C., Lim, T.M., and Kumar, P.P. 1995. IAA-induced apogamy in Platycerium coronarium (Koening) Desv. Gametophytes cultured in vitro. Plant Cell Rep 14:598–602.
Kwa, S.H., Wee, Y.C., Lim, T.M., and Kumar, P.P. 1997. Morphogenic plasticity of callus reinitiated from cell suspension cultures of the fern Platycerium coronarium. Plant Cell Tissue Organ Cult. 50:75–82.
Loescher W.H., and Albrecht C.N. 1979. Development in vitro of Nephrolepsis axaltata cv. Bostoniennsis runner tissues. Physiol Plant. 47:250–254.
Martin, K.P., Sini, S., Zhang, C.L., Slater, A., and Madhusoodanan, P.V. 2006. Efficient induction of apospory and apogamy in vitro in silver fern (Pityrogramma calomelanos L.). Plant Cell Rep. 25:1300–1307.
Materi, D.M., and Cumming, B.G. 1991. Effects of carbohydrate deprivation on rejuvenation, apospory, and regeneration in ostrich fern (Matteuccia struptiopteris) sporophyte. Can. J. Bot. 69:1241–1245.
Mehra, P.N., and Sulklyan, D.S. 1969. In vitro studies on apogamy, apospory and controlled differentiation of rhizome segments of the fern Amelopteris prolifera (Retz.) Copel. Bot. J. Linn. Soc. 62:431–443.
Menéndez, V., Revilla, M.A., and Fernández, H. 2006. Growth and gender in the gametophyte of Blechnum spicant L. Plant Cell Tissue Organs Cult. 86:47–53.
Miller, J.H. 1968. Fern gametophyte as experimental material. Bot. Rev. 34:361–440.
Mohr, H. 1962. The influence of visible radiation on the germination of archegoniate spores and the growth of the fern protonema. J. Linn. Soc. Bot. 58:287–296.
Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497
Padhya, M.A., and Mehta, A.R. 1982. Propagation of fern (Nephrolepis) through tissue culture. Plant Cell Rep. 1:261–263.
Peck, J.H., Peck, C.J., and Farrar, D.R. 1990. Influences of life history attributes on formation of local and distant fern populations. Am. Fern J. 80:126–142.
Pierik, R.M.L. 1991. Commercial aspects of micropropagation. In Horticulture- New Technologies and Applications, J. Prakash and R.M.L. Pierik, (eds.), pp. 141–153. Kluwer Academic Publishers Dordrecht, the Netherlands.
Raghavan, V. 1989. Developmental Biology of Fern Gametophytes. Cambridge University Press, Cambridge, UK.
Schneller, J.J., Haufler, C.H., and Ranker, T.A. 1990. Antheridiogen and natural gametophyte populations. Am. Fern J. 80:143–152.
Somer, M., Arbesú, R., Menéndez, V., Revilla, M.A., and Fernández, H. 2010. Sporophytes induction studies in ferns in vitro. Euphytica 171: 203–210.
Steeves, T.A. 1962. Morphogenesis in isolated fern leaves. In Regeneration, 20th Growth Symposium, D. Rudnick (ed.), Ronald Press, New York, pp. 117–151.
Teng, W.L., and Teng, M.C. 1997. In vitro regeneration patterns of Platycerium bifurcatum leaf suspension culture. Plant Cell Rep 16:820–824.
Tyron, R.M., and Vitale, G. 1977. Evidence for antheridiogen production and its mediation of a mating system in natural populations of fern gametophytes. Bot. J. Linn. Soc. 74:243–249.
von Aderkas, P. 1986. Enhancement of apospory in liquid culture of Matteuccia strupthiopteris. Ann. Bot. 57:505–510.
Wagner, W.H., and Smith A.R. 1993. Pteridolophytes. In Flora of North America North of Mexico, Flora of North America Editorial Committee (ed.), pp.247–266. Oxford University Press. New York.
Whittier, D.P. 1975. The influence of osmotic conditions on induced apogamy in Pteridium gametophytes. Phytomorphology 25:246–249.
Whittier, D.P. 2008. Red light inhibition of spore germination in Lycopodium clavatum. Am. Fern J. 98:194–198.
Whittier, D.P., and Steeves, T.A. 1960. The induction of apogamy in the bracken fern. Can. J. Bot. 38:925–930.
Whittier, D.P., and Steeves, T.A. 1962. Further studies on induced apogamy in ferns. Can. J. Bot. 40:1525–1531.
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Menéndez, V., Arbesú, R., Somer, M., Revilla, A., Fernández, H. (2011). From Spore to Sporophyte: How to Proceed In Vitro . In: Kumar, A., Fernández, H., Revilla, M. (eds) Working with Ferns. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7162-3_7
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DOI: https://doi.org/10.1007/978-1-4419-7162-3_7
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