Abstract
In the traditional Chinese system of medicine, six different ferns i.e., Drynaria fortunei, Pseudodrynaria coronans, Davallia divaricata, Davallia mariesii, Davallia solida, and Humata griffithiana are used as source of medicine commonly known as “Gu-Sui-Bu.” These have been claimed to cure inflammation, cancer, aging, blood stasis, body ache and bone injuries. However, no scientific investigation has been carried out so far, to evaluate comparative values of these sources. These plants have been over exploited for its fleshy rhizome as a source of “Gu-Sui-Bu” from its natural habitat. Hence it has become a necessity to preserve these species for future generation’s thus in vitro methodology could be the key to preserve these species. The present chapter describes in detail the comparative antioxidative potencies, scavenging activities against DPPH radical, reducing power, and estimation of polyphenol contents in six sources of “Gu-Sui-Bu” and conservation of D. fortune by in vitro methodologies.
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References
Anonymous, 1999. Zhong Hua Ben Cao (China Herbal) Vol. 2, State Adiministration of Traditional Chinese Medicine, pp. 216–264. Shanghai: Shanghai Science and Technology Press.
Anonymous, 2005. Pharmacopoeia Commission of People Republic of China (ChPC) Vol. 1, pp. 179–180. Beijing: Chemical Industry Press.
Arnous, A., Makris, D.P., and Kefalas, P. 2001. Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. J. Agric. Food Chem. 49: 5736–5742.
Benerjee, R.D. and Sen, S.P. 1980. Antibiotic activities of pteridophytes. Econ Bot. 34(2): 284–298.
Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199–1200.
Cai, Y., Luo, Q., Sun, M., and Corke, H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74: 2157–2184.
Camloh, M. 1993.Spore germination and early gametophyte development of Platycerium bifurcatum. Am Fern J. 83:124–132.
Carrol, K.K., Kurowska, E.M., and Guthirie, N. 1999. Use of citrus limonoids and flavonoids as well as tocotrienols for the treatment of cancer. Int. patent WO 9916167.
Chang E.J., Lee, W.J., Cho, S.H., and Choi, S.W. 2003. Proliferative effects of flavan-3 and propelargonidina from rhizomes of Drynaria fortunei on MCF-7 and osteblastic cells. Arch Pharm Res. 26:620–630.
Chang, H-C., Agrawal, D.C., Kuo, C-L., Wen, J-L., Chen, C-C., and Tsay, H-S. 2007a. In vitro culture of Drynaria fortunei, a fern species source of Chinese medicine “Gu-Sui-Bu.” In Vitro Cell. Deve. Biol Plant 43(2):133–139.
Chang, H-C., Huang, G.J., Agrawal, D.C., Kuo, C-L., Wu, C-R., and Tsay, H-S. 2007b. Antioxidant activities and polyphenol contents of six folk medicinal ferns used as “Gusuibu.” Bot. Stud. 48: 397–406.
Chang, W.C., Kim, S.C., Hwang, S.S., Choi, B.K., Ahn, H.J., Lee, M.Y., Park, S.H. and Kim, S.K. 2002. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci. 163: 1161–1168.
Conway, E. 1949. The autecology of bracken [Pteridium aquililinum (L.)Kuhn.]: The germination of spore, and the development of the prothallus and the young sporophyte. Proc. Roy. Soc. Edin. 63B:625–643.
Cui, C.B., Tezuka, Y., Kikuchi, T., Tamaoki, T., and Park, J.H. 1990. Constituents of fern, Davallia mariesii Moore. I. Isolution and structures of Davallialactone and a new flavanone glucuronide. Chem. Pharm. Bull. 38: 3218–3225.
Douglas, G.E. 1994. An investigation into the growth, development and ultra structure of fern gametophytes in existing and novel culture systems. PhD dissertation, University of Manchester, Manchester.
Dixit, R.D. and Vohra, J.N. 1984. A Dictionary of the Pteridophytes of India (Flora of India Series 4), Department of Environment, Government of India, pp. 1–177. Howrah. Botanical Survey of India Publication.
Duh, P.D. 1998. Antioxidant activity of Budrock (Arctium lappa Linn): its scavenging effect on free radical and active oxygen. J. Am. Oil Chem. Soc. 75: 455–461.
Edwards, M.E. and Miller, J.H., 1972a. Growth regulation by ethylene in fern gametophytes. II. Inhibition of cell division. Am. J. Bot. 59:450–457.
Edwards, M.E. and Miller, J.H., 1972b. Growth regulation by ethylene in fern gametophytes. III. Inhibition of spore germination. Am J Bot. 59:458–465.
Edwards, M.E. 1977. Carbon dioxide and ethylene control of spore germination in Onoclea sensibilis L. Plant Physiol. 59:756–758.
Frankel, E., Kanner, J., German, J., Parks, E., and Kinsella, J.E. 1993. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wines. Lancet 34: 454–457.
Giasson, B. I., Ischiropoulos, H., Lee, V. M. Y., and Trojanowski, J. Q. (2002) The relationship between oxidative/nitrosative stress and pathological inclusions in Alzheimer’s and Parkinson’s diseases. Free Radic. Biol. Med. 32:1264–1275.
Gordon, M.F. 1990. The mechanism of antioxidant action in vitro. In: Hudson, B.J.F. (ed.), Food Antioxidants. pp. 1–18. London. Elsevier Applied Science.
Halliwell, B. and Gutteridge, J.M.C. 1990. Role of free radicals and catalytic metal ions in human disease. Methods Enzymol. 186: 1–85.
Hevly, R.H. 1963. Adaptations of Cheilanthoid ferns to desert environments. J Ariz. Acad. Sci. 2:164–175.
Hwang, T.H., Kashiwada, Y., Nonaka, G.I., and Nishioka, I. 1989. Flavan-3-ol and proanthocyanidin allosides from Davallia divaricata. Phytochemistry 28: 891–896.
Ito, M. 1970. Light-induced synchrony of cell division in the protonema of fern, Pteris vittata. Planta 90:22–31.
Jayaprakasha, G.K., Singh, R.P., and Sakariah, K.K. 2002. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 73: 285–290.
Jeong, J. C., Lee, J.W., Yoon, C.H., Lee, Y.C., Chung, K.H., Kim, M.G., and Kim, C.H. 2005. Stimulative effects of Drynariae rhizoma extracts on the proliferation and differentiation of osteoblastic MC3T3-E1 cells. J. Ethnopharmacol. 96: 489–95.
Julkunen-Titto, R. 1985. Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J. Agric. Food Chem. 33: 213–217.
Kimura, K. and Noro, Y. 1965. Pharmacognostical studies on Chinese drug “Gu-sui-bu.” I. Consideration on “Gu-sui-bu” in old herbals (Pharmacognostical studies on fern drugs XI). Syoy akugaku Zasshi. 19: 25–31.
Lamaison, J.L.C. and Carnet. A. 1990. Teneurs en principaux flavonoids des fleurs de Crataegeus monogyna Jacq et de Crataegeus laevigata (Poiret D. C) en fonction de la vegetation. Pharm. Acta. Helv. 65: 315–320.
Lee, S., Suh, S., and Kim, S. 2000. Protective effects of the green tea polyphenol (-)-epigallocatechin gallate against hippocampal neuronal damage after transient global ischemia in gerbils. Neurosci. Lett. 287: 191–194.
Li, Y.-G., Tanner, G., and Larkin, P. 1996. The DMACA-HCl protocol and the threshold proanthocyanidin content for bloat safety in forage legumes. J. Sci. Food Agric. 70: 89–101.
Ma, K.C., Zhu, T.Y., and Wang, F.X. 1996. Stimulatory effects of gu-sui-bu (Drynaria baronii) injection on chick embryo bone primordium calcification in vitro. Am. J. Chin. Med. 24: 77–82.
Maeda-Yamamoto, M., Kawahara, H., Tahara, N., Tsuji, K., Hara, Y., and Isemura, M. 1999. Effects of tea polyphenols on the invasion and matrix metalloproteinases activities of human fibrosarcoma HT1080 cells. J. Agric. Food Chem. 47: 2350–2354.
Miller, N.J and Rice-Evans, C.A. 1997. Factors influencing the antioxidant activity determined by the ABTS radical cation assay. Free Radic. Res. 26: 195–199.
Mohr, H. 1956. Die Abhangigkeit des Protonemawachstums und der Protonemapolaritat bei Farnen vom Licht. Planta. 47:127–158.
Murashige. T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 15:473–497.
Pajaron, S., Pangua, E. and Gracia-Alvarez, L. 1999. Sexual expression and genetic diversity in populations of Cryptogramma crispa (Pteridaceae). Am. J. Bot. 86(7):964–973.
Parshad, R., Sanford, K.K., Price, F.M., Steele, V.E., Tarone, R.E., Kelloff, G.J., and Boone, C.W. 1998. Protective action of plant polyphenols on radiation-induced chromatid breaks in cultured human cells. Anticancer Res. 18: 3263–3266.
Pietta, P.G., Simonetti, P., and Mauri, P. 1998. Antioxidant activity of selected medicinal plants. J. Agric. Food Chem. 46: 4487–4490.
Porter, L.J., Hrstich, L.N., and Chan. B.G. 1986. The conversion of procyanidins and prodelphonidins to cyanidin and delphinidin. Phytochemistry 25: 223–230.
Ragazzi, E. and Veronese, G. 1973. Quantitative analysis of phenolics compounds after thin-layer chromatographic separation. J. Chromatogr. 77: 369–375.
Raghavan, V. 1989. Developmental biology of fern gametophytes. Cambridge: Cambridge University Press.
Raghavan, V. 1992.Germination of fern spores. Am. Sci. 80:176–185
Rice-Evans, C.A., Miller, N.J., and Paganga, G.. 1997. Antioxidant properties of phenolic compounds. Trends Plant Sci. 2: 152–159.
Sheffield, E., Douglas, G.E., Hearne, S.J., Huxham, S., and Wynn, J.M. 2001. Enhancement of fern spore germination and gametophyte growth in artificial media. Am Fern J. 91:179–186.
Soler-Rivas, C., Espin, J.C., and Wichers, H.J. 2000. An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Phytochem. Anal. 11: 330–338.
Sun, J.S., Theriault, B.L., and Anderson, G..I. 2004. The effect of Gu-Sui-Bu (Drynaria fortunei) on bone cell activity. Am. J. Chin. Med. 32: 737–753.
Swami, P and Raghavan, V. Control of morphogenesis in the gametophyte of a fern by light and growth hormones. Can. J. Bot. 1980; 58:1464–1473.
Uddin, M.G., Mirza, M.M., and Pasha, M.K. 1998. The medicinal uses of pteridophytes of Bangladesh. Bangladesh J. Plant Taxon. 5(2): 29–41.
Veglioglu, Y.S., Mazza, G., Gao, L., and Oomah. B.D. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food Chem. 46: 4113–4117.
Wayne, R. and Hepler, P.K. 1985. Red light stimulates an increase in intracellular calcium in the spores of Onoclea sensibilis L. Plant Physiol. 77:8–11.
Wayne, R. and Hepler, P.K. 1984. The role of calcium ions in phytochrome-mediated germination of spores of Onoclea sensibilis L. Planta. 160:12–20.
Whittier, D.P. 1999. Spore germination and early gametophyte development in Stromatopteris. Am Fern J. 89(2):142–148.
Whittier, D.P. 2003. The gametophyte of Diphasiastrum sitchense. Am Fern J. 93(1):20–24.
Williams, R.L. and Elliot. M.S. 1997. Antioxidants in grapes and wine: chemistry and health effects. In: F. Shaihidi (ed.), Natural Antioxidants: Chemistry, Health Effects and Applications. pp. 150–173. Champaign: AOCS Press.
Wong, R.W. and Rabie, A.B. 2006. Systemic effect of crude extract from rhizome of Drynaria fortunei on bone formation in mice. Phytother. Res. 20(4):313–315.
Zhu Y.Z., Huang, S.H., Tan, B.K.H., Sun, J., Whiteman, M., and Zhu. Y.C. 2004. Antioxidants in Chinese herbal medicines: a biochemical perspective. Nat. Prod. Rep. 21: 478–489.
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Chang, HC., Gupta, S.K., Tsay, HS. (2011). Studies on Folk Medicinal Fern: An Example of “Gu-Sui-Bu”. In: Kumar, A., Fernández, H., Revilla, M. (eds) Working with Ferns. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7162-3_21
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