Abstract
Electron tomography (ET) is the leading imaging technique for visualizing the molecular architecture of complex biological specimens. Real-time ET systems allow scientists to acquire experimental datasets and obtain a preliminary version of three-dimensional structure of the specimen. This rough structure allows assessment of the quality of the sample and can also be used as a guide to collect more datasets. However, the low signal-to-noise ratio of the ET datasets precludes detailed interpretation and makes their assessment difficult. Therefore, noise reduction methods should be integrated in these real-time ET systems for their full exploitation. However, feature-preserving noise reduction methods are typically computationally intensive, which hinders real-time response. This work proposes and evaluates fast implementations of a sophisticated noise reduction method with capabilities of preservation of biologically relevant features. These implementations are designed to exploit the high performance computing (HPC) capabilities of modern multicore platforms and of graphics processing units. It is shown that the use of HPC on modern platforms makes this noise reduction method able to provide datasets appropriate for assessment in a matter of seconds, thereby making it suitable for integration in current real-time ET systems.
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References
Fernandez JJ, Sorzano COS, Marabini R, Carazo JM (2006) Image processing and 3D reconstruction in electron microscopy. IEEE Signal Process Mag 23(3):84–94
Leis AP, Beck M, Gruska M, Best C, Hegerl R, Baumeister W, Leis JW (2006) Cryo-electron tomography of biological specimens. IEEE Signal Process Mag 23(3):95–103
Medalia O, Weber I, Frangakis AS, Nicastro D, Gerisch G, Baumeister W (2002) Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298:1209–1213
Cyrklaff M, Risco C, Fernandez JJ, Jimenez MV, Esteban M, Baumeister W, Carrascosa JL (2005) Cryo-electron tomography of vaccinia virus. Proc Natl Acad Sci USA 102:2772–2777
Schoenmakers RHM, Perquin RA, Fliervoet TF, Voorhout W, Schirmacher H (2005) New software for high resolution, high throughput electron tomography. Micros Anal 19(4):5–6
Zheng SQ, Keszthelyi B, Branlund E, Lyle JM, Braunfeld MB, Sedat JW, Agard DA (2007) UCSF tomography: An integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction. J Struct Biol 157:138–147
Fernandez JJ (2008) High performance computing in structural determination by electron cryomicroscopy. J Struct Biol 164:1–6
Frangakis AS, Hegerl R (2001) Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion. J Struct Biol 135:239–250
Fernandez JJ, Li S (2003) An improved algorithm for anisotropic nonlinear diffusion for denoising cryo-tomograms. J Struct Biol 144:152–161
Fernandez JJ, Li S (2005) Anisotropic nonlinear filtering of cellular structures in cryo-electron tomography. Comput Sci Eng 7(5):54–61
Fernandez JJ, Li S, Lucic V (2007) Three-dimensional anisotropic noise reduction with automated parameter tuning: Application to electron cryotomography. Lect Notes Comput Sci 4788:60–69
Kimmel R, Malladi R, Sochen NA (2000) Images as embedded maps and minimal surfaces: Movies, color, texture, and volumetric medical images. Int J Comput Vis 39:111–129
Fernandez JJ (2009) Tomobflow: Feature-preserving noise filtering for electron tomography. BMC Bioinformatics 10:178
Geer D (2005) Chip makers turn to multicore processors. IEEE Comput 38:11–13
Butenhof DR (1997) Programming with POSIX(R) Threads. Addison-Wesley Professional, Reading.
Nickolls J, Buck I, Garland M, Skadron K (2008) Scalable parallel programming with CUDA. ACM Queue 6:40–53
Castano-Diez D, Mueller H, Frangakis AS (2007) Implementation and performance evaluation of reconstruction algorithms on graphics processors. J Struct Biol 157:288–295
Acknowledgements
Work supported by grants MCI-TIN2008-01117, JA-P06-TIC1426 and CSIC-PIE-200920I075.
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Martínez, J.A., Fernández, J.J. (2011). Fast Three-Dimensional Noise Reduction for Real-Time Electron Tomography. In: Arabnia, H., Tran, QN. (eds) Software Tools and Algorithms for Biological Systems. Advances in Experimental Medicine and Biology, vol 696. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7046-6_23
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DOI: https://doi.org/10.1007/978-1-4419-7046-6_23
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