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Membrane Potential Imaging in the Nervous System pp 71–81Cite as

Monitoring Population Membrane Potential Signals from Neocortex

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Abstract

Voltage-sensitive dyes (VSDs) and optical imaging are useful tools for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes, we are able to record simultaneously from hundreds of points with sensitivity comparable to local field potential recordings. Since absorption dyes have less phototoxicity than fluorescent dyes, the total recording time in each slice can be 1,000–2,000 s, which can be divided into hundreds of short recording trials over several hours. In this chapter, we will discuss methodological details for achieving reliable VSD imaging with high sensitivity.

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Acknowledgments

This work was supported by NIH NS036477, NS059034, a Whitehall Foundation grant to JYW, and a fellowship grant from the American Epilepsy Society and Lennox Trust Fund to XH.

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Authors and Affiliations

  1. Department of Physiology and Biophysics, Georgetown University Medical Center, 3900 Reservoir Road MW, Washington, DC, 20007, USA

    Xiaoying Huang, Weifeng Xu, Kentaroh Takagaki & Jian-Young Wu

Authors
  1. Xiaoying Huang
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  2. Weifeng Xu
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  3. Kentaroh Takagaki
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  4. Jian-Young Wu
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Editors and Affiliations

  1. Université Joseph Fourier, Unité Inserm 836, Grenoble Institute of Neuroscience, Site Santé à la Tronche, BP 170, Grenoble, 38042 Gren, France

    Marco Canepari

  2. Dept. Cellular &, Molecular Physiology, Yale University School of Medicine, Cedar St. 333, New Haven, 06520, Connecticut, USA

    Dejan Zecevic

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Huang, X., Xu, W., Takagaki, K., Wu, JY. (2010). Monitoring Population Membrane Potential Signals from Neocortex. In: Canepari, M., Zecevic, D. (eds) Membrane Potential Imaging in the Nervous System. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-6558-5_7

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