Abstract
Studies in several important areas of neuroscience, including analysis of single neurons as well as neural networks, continue to be limited by currently available experimental tools. By combining molecular probes of cellular function, such as voltage-sensitive or calcium-sensitive dyes, with advanced microscopy techniques such as multiphoton microscopy, modern experimental neurophysiologists have been able to partially reduce this limitation. These approaches usually provide the needed spatial resolution along with convenient optical sectioning capabilities for isolating regions of interest. However, they often fall short in providing the necessary temporal resolution, primarily due to their restrained laser scanning mechanisms. In this regard, we review a method of laser scanning for multiphoton microscopy that overcomes the temporal limitations of pervious approaches and allows for what is known as Random-Access Multiphoton (RAMP) microscopy, an imaging technique that supports full three dimensional recording of many sites of interest on physiologically relevant time scales.
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Acknowledgements
The development of AOD-based fast 3D random-access multi-photon microscopy is the result of the continuing efforts of many members and affiliates of the Saggau lab. These efforts were supported by numerous grants from NIH and NSF, some of which were utilizing preliminary data obtained with equipment loaned by Coherent, Isomet Corp. and Nikon USA.
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Reddy, G.D., Saggau, P. (2010). Random-Access Multiphoton Microscopy for Fast Three-Dimensional Imaging. In: Canepari, M., Zecevic, D. (eds) Membrane Potential Imaging in the Nervous System. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-6558-5_12
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DOI: https://doi.org/10.1007/978-1-4419-6558-5_12
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