Abstract
Phox2b expressing cells in the parafacial region of the ventral medulla are proposed to play a role in central chemoreception and postnatal survival. We have showed that pre-inspiratory (Pre-I) neurons in the parafacial region (pFRG/Pre-I) in neonatal rats express Phox2b and are postsynaptically CO2 sensitive. Recent evidence examining Phox2b expression established more clearly the cell architecture of the pFRG, and that Phox2b expression is the most noticeable characteristic of pFRG/Pre-I neurons. Here, we briefly review these recent findings. We also show a unique distribution of Phox2b expressing cells in the most rostral medulla, which could be an interesting target for neurophysiological analysis.
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References
Ballanyi, K., Onimaru, H., and Homma, I. (1999) Respiratory network function in the isolated brainstem-spinal cord of newborn rats. Prog. Neurobiol. 59, 583–634.
Connelly, C.A., Ellenberger, H.H., and Feldman, J.L. (1990) Respiratory activity in retrotrapezoid nucleus in cat. Am. J. Physiol. 258, L33–L44.
Dubreuil, V., Ramanantsoa, N., Trochet, D., Vaubourg, V., Amiel, J., Gallego, J., Brunet, J.F., and Goridis, C. (2008) A human mutation in Phox2b causes lack of CO2 chemosensitivity, fatal central apnea, and specific loss of parafacial neurons. Proc. Natl. Acad. Sci. USA. 105, 1067-1072.
Ellenberger, H.H. and Feldman, J.L. (1990) Brainstem connections of the rostral ventral respiratory group of the rat. Brain Res. 513, 35–42.
Guyenet, P.G., Stornetta, R.L., and Bayliss, D.A. (2008) Retrotrapezoid nucleus and central chemoreception. J. Physiol. 586, 2043–2048.
Kang, B.J., Chang, D.A., Mackay, D.D., West, G.H., Moreira, T.S., Takakura, A.C., Gwilt, J.M., Guyenet, P.G., and Stornetta, R.L. (2007) Central nervous system distribution of the transcription factor Phox2b in the adult rat. J. Comp. Neurol. 503, 627–641.
Kawai, A., Onimaru, H., and Homma, I. (2006) Mechanisms of CO2/H+ chemoreception by respiratory rhythm generator neurons in the medulla from newborn rats in vitro. J. Physiol. 572, 525–537.
Nunez-Abades, P.A., Morillo, A.M., and Pasaro, R. (1993) Brainstem connections of the rat ventral respiratory subgroups: afferent projections. J. Auton. Nerv. Syst. 42, 99–118.
Onimaru, H. and Homma, I. (1992) Whole cell recordings from respiratory neurons in the medulla of brainstem-spinal cord preparations isolated from newborn rats. Pflugers Arch. 420, 399–406.
Onimaru, H. and Homma, I. (2003) A novel functional neuron group for respiratory rhythm generation in the ventral medulla. J. Neurosci. 23, 1478–1486.
Onimaru, H., Ikeda, K., and Kawakami, K. (2008) CO2-sensitive preinspiratory neurons of the parafacial respiratory group express Phox2b in the neonatal rat. J. Neurosci. 28, 12845–12850.
Onimaru, H., Ikeda, K., and Kawakami, K. (2009). Phox2b, RTN/pFRG neurons and respiratory rhythmogenesis. Respir. Physiol. Neurobiol. 168, 13–18.
Pearce, R.A., Stornetta, R.L., and Guyenet, P.G. (1989). Retrotrapezoid nucleus in the rat. Neurosci. Lett. 101, 138–142.
Smith, J.C., Morrison, D.E., Ellenberger, H.H., Otto, M.R., and Feldman, J.L. (1989) Brainstem projections to the major respiratory neuron populations in the medulla of the cat. J. Comp. Neurol. 281, 69–96.
Stornetta, R.L., Moreira, T.S., Takakura, A.C., Kang, B.J., Chang, D.A., West, G.H., Brunet, J.F., Mulkey, D.K., Bayliss, D.A., and Guyenet, P.G. (2006) Expression of Phox2b by brainstem neurons involved in chemosensory integration in the adult rat. J. Neurosci. 26, 10305–10314.
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Onimaru, H., Ikeda, K., Kawakami, K. (2010). Phox2b Expressing Neurons in the Most Rostral Medulla of Newborn Rats. In: Homma, I., Onimaru, H., Fukuchi, Y. (eds) New Frontiers in Respiratory Control. Advances in Experimental Medicine and Biology, vol 669. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5692-7_18
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DOI: https://doi.org/10.1007/978-1-4419-5692-7_18
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