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Advances in Bioanalysis as It Relates to ADME

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Drug Metabolism and Pharmacokinetics Quick Guide

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Abstract

Bioanalysis continues to play a crucial role in ADME sciences. Most in vitro or in vivo studies have either a quantitative or qualitative bioanalytical endpoint. This chapter provides a general overview of the use of mass spectrometry in ADME sciences and the advantages and disadvantages of the various types of instruments.

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Abbreviations

ADME:

Absorption, distribution, metabolism, and excretion

APCI:

Atmospheric pressure chemical ionization

API:

Atmospheric pressure ionization

APPI:

Atmospheric pressure photo ionization

DART:

Direct analysis in real time

DBS:

Dried blood spot

DESI:

Desorption electrospray ionization

ESI:

Electrospray ionization

GC:

Gas chromatography

GLP:

Good laboratory practice

HILIC:

Hydrophilic interaction liquid chromatography

HPLC:

High performance liquid chromatography

LC:

Liquid chromatography

MALDI:

Matrix-assisted laser desorption/ionization

MIM:

Multiple ion monitoring

MRM:

Multiple reaction monitoring

MS:

Mass spectroscopy

P450:

Cytochrome P450

PD:

Pharmacodynamic

PK:

Pharmacokinetic

SIM:

Selected ion monitoring

SRM:

Selected reaction monitoring

UHPLC:

Ultra high performance liquid chromatography or ultra high pressure liquid chromatography

UPLC:

Ultra performance liquid chromatography

UV:

Ultraviolet

References

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Additional Readings

  • Chowdhury SK (2005) Identification and quantification of drugs, metabolites and metabolizing enzymes by LC-MS. Elsevier, Amsterdam, The Netherlands

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  • Hop CECA (2006) LC-MS in drug disposition and metabolism. In: Caprioli RM (ed) The encyclopedia of mass spectrometry, vol 3. The Netherlands, Elsevier, Amsterdam, pp 233–274

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  • Korfmacher WA (2010) Using mass spectrometry for drug metabolism studies, 2nd edn. CRC Press, Boca Raton, FL

    Google Scholar 

  • Ramanathan R (2009) Mass spectrometry in drug metabolism and pharmacokinetics. Wiley, New York

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Author information

Authors and Affiliations

  1. Genentech, Inc., 1 DNA Way, San Francisco, California, USA

    Siamak Cyrus Khojasteh, Harvey Wong & Cornelis E. C. A. Hop

Authors
  1. Siamak Cyrus Khojasteh
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  2. Harvey Wong
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  3. Cornelis E. C. A. Hop
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Corresponding author

Correspondence to Siamak Cyrus Khojasteh .

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© 2011 Springer Science+Business Media, LLC

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Khojasteh, S.C., Wong, H., Hop, C.E.C.A. (2011). Advances in Bioanalysis as It Relates to ADME. In: Drug Metabolism and Pharmacokinetics Quick Guide. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5629-3_8

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