Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence

Chapter

Abstract

The occurrence of an intrafollicular biochemistry that can be used to relate oocyte developmental competence with quantitative and qualitative aspects of specific components of ­follicular fluid aspirated at ovum retrieval has been a goal of research in clinical IVF for decades. As a complex mixture of bioactive molecules, some produced in situ and others serum borne, there is no shortage of candidates for investigation. This chapter focuses on current methods of high-resolution biochemical analysis that can be applied to follicular biochemistry, which candidates may be the most promising in this regard, and specific investigational approaches that may have the greatest potential to fulfill this goal. In particular, emphasis is placed on whether there is a need to know both the target(s) and function(s) of candidate molecules as related to the developmental biology of the oocyte.

Keywords

Molecular mining of follicular fluid Biomarkers of human oocyte development Microanalytical imaging Genetic and biochemical technologies in reproduction Follicular fluid markers 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of ColoradoBoulderUSA

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