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Polscope-Based Sperm Selection

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Practical Manual of In Vitro Fertilization

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Abstract

In a time in which the severe male factor affects at least 50% of infertile couples and ICSI is becoming more and more necessary, refining the selection criteria that help identifying the fertilizing spermatozoon might substantially contribute to the establishment of a healthy pregnancy. The properties of birefringence in the sperm head seem to represent a novel approach aimed at maximizing the viability of the resulting embryos. The clinical data reported are promising and support further application of this strategy.

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References

  1. Van Assche EV, Bounduelle M, Tournaye H, Joris H, Verheyen A, Devroey P, Van Steirteghem A, Libaers I. Cytogenetics of infertile men. Hum Reprod. 1996;11:1–26.

    Article  PubMed  Google Scholar 

  2. Yoshida A, Miura K, Shirai M. Chromosome abnormalities and male infertility. Assist Reprod Rev. 1996;67:93–9.

    Google Scholar 

  3. Egozcue S, Blanco J, Vendrell JM, Gracia F, Veiga A, Aran B, Barri PN, Vidal F, Egozcue J. Human male infertility: chromosome anomalies, meiotic disorder, abnormal spermatozoa and recurrent abortion. Hum Reprod Update. 2000;6:93–105.

    Article  PubMed  CAS  Google Scholar 

  4. Bernardini LM, Costa M, Bottazzi C, Gianaroli L, Magli MC, Venturini PL, Francioso R, Conte N, Ragni N. Sperm aneuploidy and recurrent pregnancy loss. Reprod Biomed Online. 2004;9:312–20.

    Article  PubMed  CAS  Google Scholar 

  5. Bernardini LM, Calogero AE, Bottazzi C, Lanteri S, Venturini PL, Burello N, De Palma A, Conte N, Ragni N. Low total normal motile count values are associated with increased sperm disomy and diploidy retes in infertile patients. Int J Androl. 2005;28:328–36.

    Article  PubMed  Google Scholar 

  6. Koehler KE, Hawley RS, Sherman S, Hassold T. Recombination and non-disjunction in humans and flies. Hum Mol Genet. 1996;5:1495–504.

    PubMed  CAS  Google Scholar 

  7. Sathananthan AH, Kola I, Osborne J, Trounson A, Ng SC, Bongso A, Ratnam SS. Centrioles in the beginning of human development. Proc Natl Acad Sci U S A. 1991;88:4806–910.

    Article  PubMed  CAS  Google Scholar 

  8. Palermo G, Munné S, Cohen J. The human zygote inherits its mitotic potential from the male gamete. Hum Reprod. 1994;9:1220–5.

    PubMed  CAS  Google Scholar 

  9. Silber S, Escudero T, Lenahan K, Abdelhadi I, Kilani Z, Munné S. Chromosomal abnormalities in embryos derived from testicular sperm extraction. Fertil Steril. 2003;79:30–8.

    Article  PubMed  Google Scholar 

  10. Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y. Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl. 2002;23:1–8.

    PubMed  Google Scholar 

  11. Calogero AE, Burrello N, De Palma A, Barone N, D’Agata R, Vicari E. Sperm aneuploidy in infertile men. Reprod Biomed Online. 2003;6:310–7.

    Article  PubMed  CAS  Google Scholar 

  12. Gianaroli L, Magli MC, Cavallini G, Crippa A, Nadalini M, Bernardini L, Menchini-Fabris GF, Voliani S, Ferraretti AP. Frequency of aneuploidy in spermatozoa from patients with extremely severe male factor infertility. Hum Reprod. 2005;20: 2140–52.

    Article  PubMed  Google Scholar 

  13. Gianaroli L, Magli MC, Ferraretti AP. Sperm and blastomere aneuploidy detection in reproductive genetics and medicine. J Histochem Cytochem. 2005;53:261–8.

    Article  PubMed  CAS  Google Scholar 

  14. Baccetti B. Microscopical advances in assisted reproduction. J Submicrosc Cytol Pathol. 2004;36:333–9.

    PubMed  CAS  Google Scholar 

  15. Gianaroli L, Magli MC, Collodel G, Moretti E, Ferraretti AP, Baccetti B. Sperm head’s birefringence: a new criterion for sperm selection. Fertil Steril. 2008;90:104–12.

    Article  PubMed  Google Scholar 

  16. Gianaroli L, Magli MC, Ferraretti AP, Crippa A, Lappi M, Capitani S, Baccetti B. Birefringence characteristics in sperm heads allow for the selection of reacted spermatozoa for intracytoplasmic sperm injection. Fertil Steril. 2010;93:807–13.

    Article  PubMed  Google Scholar 

  17. Chattopadhyay R, Ghosh S, Goswami SK, Goswami M. Selection of birefringent sperm head under polscope and its effect on outcome of ICSI in azoospermia and complete asthenozoospermia. Hum Reprod. 2009;24 Suppl 1:i71.

    Google Scholar 

  18. Boudjema E, Magli MC, Crippa A, Baccetti B, Ferraretti AP, Gianaroli L. Correlation between sperm morphology and birefringence properties in human spermatozoa: implications for sperm selection at ICSI. Hum Reprod. 2009;24 Suppl 1:i72.

    Google Scholar 

  19. Crippa A, Gianaroli L, Ferraretti AP, Baccetti B, Cetera C, Magli MC. Chromosomal status and characteristics of birefringence in sperm cell heads. Hum Reprod. 2008;23 Suppl 1:i112.

    Google Scholar 

  20. Crippa A, Magli MC, Paviglianiti B, Boudjema E, Ferraretti AP, Gianaroli L. DNA fragmentation and characteristics of birefringence in human sperm head. Hum Reprod. 2009;24 Suppl 1:i95.

    Google Scholar 

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Authors and Affiliations

  1. International Institutes of Advanced Reproduction and Genetics, SISMER, Via Mazzini 12, Bolgona, 40138, Italy

    Luca Gianaroli MD

  2. Research and Development, SISMER, Bolgona, Italy

    Cristina Magli MSc

  3. Andrology Laboratory and Genetics, SISMER, Bolgona, Italy

    Andor Crippa PhD

  4. Reproductive Medicine Unit, SISMER, Bolgona, Italy

    Giorgio Cavallini MD & Anna P. Ferraretti MD

  5. Andrology Laboratory, SISMER, Bolgona, Italy

    Eleonora Borghi BSc

Authors
  1. Luca Gianaroli MD
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  2. Cristina Magli MSc
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  3. Andor Crippa PhD
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  4. Giorgio Cavallini MD
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  5. Eleonora Borghi BSc
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  6. Anna P. Ferraretti MD
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Corresponding author

Correspondence to Luca Gianaroli MD .

Editor information

Editors and Affiliations

  1. , Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, 30342, Georgia, USA

    Zsolt Peter Nagy

  2. , Sr. Embryologist, Montreal Reproductive Centre, Montreal, H4A 3J3, Québec, Canada

    Alex C. Varghese

  3. Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Ashok Agarwal

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© 2012 Springer Science+Business Media, LLC

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Gianaroli, L., Magli, C., Crippa, A., Cavallini, G., Borghi, E., Ferraretti, A.P. (2012). Polscope-Based Sperm Selection. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Practical Manual of In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1780-5_30

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  • DOI: https://doi.org/10.1007/978-1-4419-1780-5_30

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4419-1779-9

  • Online ISBN: 978-1-4419-1780-5

  • eBook Packages: MedicineMedicine (R0)

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