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Extended Culture in IVF

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Practical Manual of In Vitro Fertilization

Abstract

With the advent of culture media based upon the nutrient composition of the human oviduct and uterus, and specifically designed to support the human embryo in vitro, it has become feasible to culture the pronucleate oocyte to the blastocyst stage as a routine procedure in human IVF. The potential advantages of extended culture go beyond increases in implantation and pregnancy rates and include lower miscarriage rates and the ability to perform comprehensive genetic and physiological analysis of the embryo proper prior to transfer. The capacity to better select a viable embryo will continue to improve the safety of IVF to the mother and child without a compromise in efficacy. The ability of blastocysts to undergo successful cryopreservation with vitrification will culminate in the transfer of genetically analyzed embryos to a naturally cycling uterus. The transfer of vitrified blastocysts in a nonstimulated cycle may ultimately be the way all future IVF is performed.

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Authors and Affiliations

  1. Department of Zoology, University of Melbourne, Parkville, VIC, 3010, Australia

    David K. Gardner PhD

  2. Repromed, Dulwich, Adelaide, SA, Australia

    Michelle Lane BSc, PhD

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  1. David K. Gardner PhD
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Correspondence to David K. Gardner PhD .

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Editors and Affiliations

  1. , Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, 30342, Georgia, USA

    Zsolt Peter Nagy

  2. , Sr. Embryologist, Montreal Reproductive Centre, Montreal, H4A 3J3, Québec, Canada

    Alex C. Varghese

  3. Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Ashok Agarwal

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Gardner, D.K., Lane, M. (2012). Extended Culture in IVF. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Practical Manual of In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1780-5_17

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