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Culture Media in IVF: Decisions for the Laboratory

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Practical Manual of In Vitro Fertilization

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Abstract

Appreciation of the evolution of IVF culture media, highlighting salient discoveries that have led to the tremendous improvement over just a few short years ago, is instrumental in gaining insight into the complexities of gamete and embryo function. In turn, this knowledge brings understanding to the rationale behind current laboratory practices and aids in the ability to make informed decisions in regard to culture methods. Furthermore, discussion of impact of culture media on homeostatic regulation of gametes and embryos, focusing on key decisions made within the laboratory such as media type, macromolecule selection, and pH, further highlights their delicate nature, the need to minimize stressors, and ultimately provides insight into areas where future improvement can be made as we continue to strive for improvement in IVF success rates.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA

    Jason E. Swain PhD, HCLD

  2. Fertility Center of Antonio, San Antonio, TX, USA

    Thomas B. Pool PhD, HCLD (ABB)

Authors
  1. Jason E. Swain PhD, HCLD
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  2. Thomas B. Pool PhD, HCLD (ABB)
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Correspondence to Jason E. Swain PhD, HCLD .

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Editors and Affiliations

  1. , Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, 30342, Georgia, USA

    Zsolt Peter Nagy

  2. , Sr. Embryologist, Montreal Reproductive Centre, Montreal, H4A 3J3, Québec, Canada

    Alex C. Varghese

  3. Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Ashok Agarwal

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Swain, J.E., Pool, T.B. (2012). Culture Media in IVF: Decisions for the Laboratory. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Practical Manual of In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1780-5_11

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