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Genetics and Genomics of Populus pp 113–133Cite as

Transformation as a Tool for Genetic Analysis in Populus

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Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 8))

Abstract

We summarize the outlook for using transformation as a genetic tool in Populus. Transformation approaches avoid the major obstacle to performing genetics experiments in trees – namely long generation cycles and the difficulty of inbreeding to reveal loss of function alleles. Dominant transgenic alleles allow modifications in gene function to be readily observed in primary transformants. Although transformation has been mainly used for reverse genetics (where the gene sequence of interest is known), transgenic mutagenesis approaches such as activation tagging and gene/enhancer traps have also been shown to enable forward genetics (where the phenotype, not the gene, is known). We outline challenges and needs for more efficient use of transformation tools. These include expansion of the transformation toolbox (e.g., promoters, vectors, targeting), and improved ability to conduct field trialsto study gene function in native and plantation environments (in spite of regulatory obstacles). Because of the power of transformation, it will remain a major genetic research tool for dissection of gene function in Populus for many years to come. It is the key biological attribute that makes poplar the most powerful model organism for genetic analysis of woody plant growth, adaptation, and development.

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Authors and Affiliations

  1. School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI, 49931, USA

    Victor B. Busov

  2. Department of Forest Ecosystems and Society, Oregon State University, Corvallis, OR, 97331-5752, USA

    Steven H. Strauss

  3. INRA Orléans, Avenue de la Pomme de Pin, BP 20 619 Ardon, F-45166, Olivet, Cédex, France

    Gilles Pilate

Authors
  1. Victor B. Busov
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  2. Steven H. Strauss
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  3. Gilles Pilate
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Corresponding author

Correspondence to Victor B. Busov .

Editor information

Editors and Affiliations

  1. Dept. Plant Physiology, University of Umea, Umea, 901 87, Sweden

    Stefan Jansson

  2. Agricultural Sciences, Swedish University of, Umea, 901 83, Sweden

    Rishikesh Bhalerao

  3. Inst. Forest Genetics &, USDA Forest Service, Davis, 95618, U.S.A.

    Andrew Groover

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© 2010 Springer Science+Business Media, LLC

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Busov, V.B., Strauss, S.H., Pilate, G. (2010). Transformation as a Tool for Genetic Analysis in Populus . In: Jansson, S., Bhalerao, R., Groover, A. (eds) Genetics and Genomics of Populus. Plant Genetics and Genomics: Crops and Models, vol 8. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1541-2_6

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